E15.5 Mouse Embryo Micro-CT Using a Bruker Skyscan 1172 Micro-CT

X-ray computed microtomography (µCT) is a powerful tool to reveal the 3D structure of tissues and organs. Compared with the traditional sectioning, staining, and microscopy image acquisition, it allows a better understanding of the morphology and a precise morphometric analysis. Here, we describe a...

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Detalles Bibliográficos
Autores principales: Astanina, Elena, Petrillo, Sara, Genova, Tullio, Mussano, Federico, Bussolino, Federico
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bio-Protocol 2023
Materias:
Methods Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10176208/
https://www.ncbi.nlm.nih.gov/pubmed/37188107
http://dx.doi.org/10.21769/BioProtoc.4662
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author Astanina, Elena
Petrillo, Sara
Genova, Tullio
Mussano, Federico
Bussolino, Federico
author_facet Astanina, Elena
Petrillo, Sara
Genova, Tullio
Mussano, Federico
Bussolino, Federico
author_sort Astanina, Elena
collection PubMed
description X-ray computed microtomography (µCT) is a powerful tool to reveal the 3D structure of tissues and organs. Compared with the traditional sectioning, staining, and microscopy image acquisition, it allows a better understanding of the morphology and a precise morphometric analysis. Here, we describe a method for 3D visualization and morphometric analysis by µCT scanning of the embryonic heart of iodine-stained E15.5 mouse embryos.
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spelling pubmed-101762082023-05-13 E15.5 Mouse Embryo Micro-CT Using a Bruker Skyscan 1172 Micro-CT Astanina, Elena Petrillo, Sara Genova, Tullio Mussano, Federico Bussolino, Federico Bio Protoc Methods Article X-ray computed microtomography (µCT) is a powerful tool to reveal the 3D structure of tissues and organs. Compared with the traditional sectioning, staining, and microscopy image acquisition, it allows a better understanding of the morphology and a precise morphometric analysis. Here, we describe a method for 3D visualization and morphometric analysis by µCT scanning of the embryonic heart of iodine-stained E15.5 mouse embryos. Bio-Protocol 2023-05-05 /pmc/articles/PMC10176208/ /pubmed/37188107 http://dx.doi.org/10.21769/BioProtoc.4662 Text en Copyright © 2023 The Authors; exclusive licensee Bio-protocol LLC. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the CC BY-NC license (https://creativecommons.org/licenses/by-nc/4.0/).
spellingShingle Methods Article
Astanina, Elena
Petrillo, Sara
Genova, Tullio
Mussano, Federico
Bussolino, Federico
E15.5 Mouse Embryo Micro-CT Using a Bruker Skyscan 1172 Micro-CT
title E15.5 Mouse Embryo Micro-CT Using a Bruker Skyscan 1172 Micro-CT
title_full E15.5 Mouse Embryo Micro-CT Using a Bruker Skyscan 1172 Micro-CT
title_fullStr E15.5 Mouse Embryo Micro-CT Using a Bruker Skyscan 1172 Micro-CT
title_full_unstemmed E15.5 Mouse Embryo Micro-CT Using a Bruker Skyscan 1172 Micro-CT
title_short E15.5 Mouse Embryo Micro-CT Using a Bruker Skyscan 1172 Micro-CT
title_sort e15.5 mouse embryo micro-ct using a bruker skyscan 1172 micro-ct
topic Methods Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10176208/
https://www.ncbi.nlm.nih.gov/pubmed/37188107
http://dx.doi.org/10.21769/BioProtoc.4662
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