Cargando…

Triggered Golgi membrane enrichment promotes PtdIns(4,5)P2 generation for plasma membrane repair

The maintenance of plasma membrane integrity and a capacity for efficiently repairing damaged membranes are essential for cell survival. Large-scale wounding depletes various membrane components at the wound sites, including phosphatidylinositols, yet little is known about how phosphatidylinositols...

Descripción completa

Detalles Bibliográficos
Autores principales: Meng, Xinan, Wijaya, Chandra Sugiarto, Shao, Qingfang, Xu, Suhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10176212/
https://www.ncbi.nlm.nih.gov/pubmed/37158801
http://dx.doi.org/10.1083/jcb.202303017
_version_ 1785040383136235520
author Meng, Xinan
Wijaya, Chandra Sugiarto
Shao, Qingfang
Xu, Suhong
author_facet Meng, Xinan
Wijaya, Chandra Sugiarto
Shao, Qingfang
Xu, Suhong
author_sort Meng, Xinan
collection PubMed
description The maintenance of plasma membrane integrity and a capacity for efficiently repairing damaged membranes are essential for cell survival. Large-scale wounding depletes various membrane components at the wound sites, including phosphatidylinositols, yet little is known about how phosphatidylinositols are generated after depletion. Here, working with our in vivo C. elegans epidermal cell wounding model, we discovered phosphatidylinositol 4-phosphate (PtdIns4P) accumulation and local phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P(2)] generation at the wound site. We found that PtdIns(4,5)P(2) generation depends on the delivery of PtdIns4P, PI4K, and PI4P 5-kinase PPK-1. In addition, we show that wounding triggers enrichment of the Golgi membrane to the wound site, and that is required for membrane repair. Moreover, genetic and pharmacological inhibitor experiments support that the Golgi membrane provides the PtdIns4P for PtdIns(4,5)P(2) generation at the wounds. Our findings demonstrate how the Golgi apparatus facilitates membrane repair in response to wounding and offers a valuable perspective on cellular survival mechanisms upon mechanical stress in a physiological context.
format Online
Article
Text
id pubmed-10176212
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-101762122023-05-13 Triggered Golgi membrane enrichment promotes PtdIns(4,5)P2 generation for plasma membrane repair Meng, Xinan Wijaya, Chandra Sugiarto Shao, Qingfang Xu, Suhong J Cell Biol Report The maintenance of plasma membrane integrity and a capacity for efficiently repairing damaged membranes are essential for cell survival. Large-scale wounding depletes various membrane components at the wound sites, including phosphatidylinositols, yet little is known about how phosphatidylinositols are generated after depletion. Here, working with our in vivo C. elegans epidermal cell wounding model, we discovered phosphatidylinositol 4-phosphate (PtdIns4P) accumulation and local phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P(2)] generation at the wound site. We found that PtdIns(4,5)P(2) generation depends on the delivery of PtdIns4P, PI4K, and PI4P 5-kinase PPK-1. In addition, we show that wounding triggers enrichment of the Golgi membrane to the wound site, and that is required for membrane repair. Moreover, genetic and pharmacological inhibitor experiments support that the Golgi membrane provides the PtdIns4P for PtdIns(4,5)P(2) generation at the wounds. Our findings demonstrate how the Golgi apparatus facilitates membrane repair in response to wounding and offers a valuable perspective on cellular survival mechanisms upon mechanical stress in a physiological context. Rockefeller University Press 2023-05-09 /pmc/articles/PMC10176212/ /pubmed/37158801 http://dx.doi.org/10.1083/jcb.202303017 Text en © 2023 Meng et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
spellingShingle Report
Meng, Xinan
Wijaya, Chandra Sugiarto
Shao, Qingfang
Xu, Suhong
Triggered Golgi membrane enrichment promotes PtdIns(4,5)P2 generation for plasma membrane repair
title Triggered Golgi membrane enrichment promotes PtdIns(4,5)P2 generation for plasma membrane repair
title_full Triggered Golgi membrane enrichment promotes PtdIns(4,5)P2 generation for plasma membrane repair
title_fullStr Triggered Golgi membrane enrichment promotes PtdIns(4,5)P2 generation for plasma membrane repair
title_full_unstemmed Triggered Golgi membrane enrichment promotes PtdIns(4,5)P2 generation for plasma membrane repair
title_short Triggered Golgi membrane enrichment promotes PtdIns(4,5)P2 generation for plasma membrane repair
title_sort triggered golgi membrane enrichment promotes ptdins(4,5)p2 generation for plasma membrane repair
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10176212/
https://www.ncbi.nlm.nih.gov/pubmed/37158801
http://dx.doi.org/10.1083/jcb.202303017
work_keys_str_mv AT mengxinan triggeredgolgimembraneenrichmentpromotesptdins45p2generationforplasmamembranerepair
AT wijayachandrasugiarto triggeredgolgimembraneenrichmentpromotesptdins45p2generationforplasmamembranerepair
AT shaoqingfang triggeredgolgimembraneenrichmentpromotesptdins45p2generationforplasmamembranerepair
AT xusuhong triggeredgolgimembraneenrichmentpromotesptdins45p2generationforplasmamembranerepair