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Triggered Golgi membrane enrichment promotes PtdIns(4,5)P2 generation for plasma membrane repair
The maintenance of plasma membrane integrity and a capacity for efficiently repairing damaged membranes are essential for cell survival. Large-scale wounding depletes various membrane components at the wound sites, including phosphatidylinositols, yet little is known about how phosphatidylinositols...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Rockefeller University Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10176212/ https://www.ncbi.nlm.nih.gov/pubmed/37158801 http://dx.doi.org/10.1083/jcb.202303017 |
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author | Meng, Xinan Wijaya, Chandra Sugiarto Shao, Qingfang Xu, Suhong |
author_facet | Meng, Xinan Wijaya, Chandra Sugiarto Shao, Qingfang Xu, Suhong |
author_sort | Meng, Xinan |
collection | PubMed |
description | The maintenance of plasma membrane integrity and a capacity for efficiently repairing damaged membranes are essential for cell survival. Large-scale wounding depletes various membrane components at the wound sites, including phosphatidylinositols, yet little is known about how phosphatidylinositols are generated after depletion. Here, working with our in vivo C. elegans epidermal cell wounding model, we discovered phosphatidylinositol 4-phosphate (PtdIns4P) accumulation and local phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P(2)] generation at the wound site. We found that PtdIns(4,5)P(2) generation depends on the delivery of PtdIns4P, PI4K, and PI4P 5-kinase PPK-1. In addition, we show that wounding triggers enrichment of the Golgi membrane to the wound site, and that is required for membrane repair. Moreover, genetic and pharmacological inhibitor experiments support that the Golgi membrane provides the PtdIns4P for PtdIns(4,5)P(2) generation at the wounds. Our findings demonstrate how the Golgi apparatus facilitates membrane repair in response to wounding and offers a valuable perspective on cellular survival mechanisms upon mechanical stress in a physiological context. |
format | Online Article Text |
id | pubmed-10176212 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-101762122023-05-13 Triggered Golgi membrane enrichment promotes PtdIns(4,5)P2 generation for plasma membrane repair Meng, Xinan Wijaya, Chandra Sugiarto Shao, Qingfang Xu, Suhong J Cell Biol Report The maintenance of plasma membrane integrity and a capacity for efficiently repairing damaged membranes are essential for cell survival. Large-scale wounding depletes various membrane components at the wound sites, including phosphatidylinositols, yet little is known about how phosphatidylinositols are generated after depletion. Here, working with our in vivo C. elegans epidermal cell wounding model, we discovered phosphatidylinositol 4-phosphate (PtdIns4P) accumulation and local phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P(2)] generation at the wound site. We found that PtdIns(4,5)P(2) generation depends on the delivery of PtdIns4P, PI4K, and PI4P 5-kinase PPK-1. In addition, we show that wounding triggers enrichment of the Golgi membrane to the wound site, and that is required for membrane repair. Moreover, genetic and pharmacological inhibitor experiments support that the Golgi membrane provides the PtdIns4P for PtdIns(4,5)P(2) generation at the wounds. Our findings demonstrate how the Golgi apparatus facilitates membrane repair in response to wounding and offers a valuable perspective on cellular survival mechanisms upon mechanical stress in a physiological context. Rockefeller University Press 2023-05-09 /pmc/articles/PMC10176212/ /pubmed/37158801 http://dx.doi.org/10.1083/jcb.202303017 Text en © 2023 Meng et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Report Meng, Xinan Wijaya, Chandra Sugiarto Shao, Qingfang Xu, Suhong Triggered Golgi membrane enrichment promotes PtdIns(4,5)P2 generation for plasma membrane repair |
title | Triggered Golgi membrane enrichment promotes PtdIns(4,5)P2 generation for plasma membrane repair |
title_full | Triggered Golgi membrane enrichment promotes PtdIns(4,5)P2 generation for plasma membrane repair |
title_fullStr | Triggered Golgi membrane enrichment promotes PtdIns(4,5)P2 generation for plasma membrane repair |
title_full_unstemmed | Triggered Golgi membrane enrichment promotes PtdIns(4,5)P2 generation for plasma membrane repair |
title_short | Triggered Golgi membrane enrichment promotes PtdIns(4,5)P2 generation for plasma membrane repair |
title_sort | triggered golgi membrane enrichment promotes ptdins(4,5)p2 generation for plasma membrane repair |
topic | Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10176212/ https://www.ncbi.nlm.nih.gov/pubmed/37158801 http://dx.doi.org/10.1083/jcb.202303017 |
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