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Ginger extract suppresses the activations of NF-κB and Wnt pathways and protects inflammatory arthritis

OBJECTIVE: Rheumatoid arthritis (RA) is a disabling inflammatory disorder. Ginger is used for food and medicine to treat arthralgia, sprains, and muscle aches. Anti-inflammatory effects of ginger have been observed. The aim of our study was to detect the effects of ginger on experimentally induced i...

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Autores principales: Öz, Burak, Orhan, Cemal, Tuzcu, Mehmet, Şahin, Nurhan, Özercan, İbrahim Hanifi, Öner, Pınar Demirel, Serdar Koca, Süleyman, Juturu, Vijaya, Şahin, Kazım
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medical Research and Education Association 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10176230/
https://www.ncbi.nlm.nih.gov/pubmed/35110180
http://dx.doi.org/10.5152/eujrheum.2020.20192
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author Öz, Burak
Orhan, Cemal
Tuzcu, Mehmet
Şahin, Nurhan
Özercan, İbrahim Hanifi
Öner, Pınar Demirel
Serdar Koca, Süleyman
Juturu, Vijaya
Şahin, Kazım
author_facet Öz, Burak
Orhan, Cemal
Tuzcu, Mehmet
Şahin, Nurhan
Özercan, İbrahim Hanifi
Öner, Pınar Demirel
Serdar Koca, Süleyman
Juturu, Vijaya
Şahin, Kazım
author_sort Öz, Burak
collection PubMed
description OBJECTIVE: Rheumatoid arthritis (RA) is a disabling inflammatory disorder. Ginger is used for food and medicine to treat arthralgia, sprains, and muscle aches. Anti-inflammatory effects of ginger have been observed. The aim of our study was to detect the effects of ginger on experimentally induced inflammatory arthritis. METHODS: Female Wistar albino rats (n = 21) were randomly separated into three groups (control, arthritis, and arthritis + ginger). Arthritis was generated by an appropriate method using type 2 collagen and Freund's adjuvant (collagen-induced arthritis model). The ginger group was treated starting at the first collagen injection with ginger root extract for 32 days by oral gavage (50 mg/kg/daily). Interleukin (IL)-6, IL-17, tumor necrosis factor-α (TNF-α), sclerostin, dickkopf-related protein-1 (DKK-1), and obestatin serum levels were studied by enzyme-linked immunosorbent assay method. Tissue TNF-α, IL-17, cyclooxygenase-2 (COX-2), and nuclear factor kappa B (NF-κB) levels were detected using the Western blot method. RESULTS: Mean arthritis score and serum levels of TNF-α, IL-6, and IL-17 were significantly decreased in ginger group than in the arthritis group. Increased sclerostin serum level and decreased DKK-1 serum levels were detected in ginger group compared with arthritis group. The decreases of IL-17, TNF-α, COX-2, and NF-κB tissue levels were statistically significant in the ginger group compared with arthritis group. Histopathological evaluation of the ginger group showed a decrease in the inflammation score compared to arthritis group. CONCLUSION: It can be concluded that ginger has protective properties in the development of inflammatory arthritis. The antiarthritic acts of ginger are related to NF-κB activity and Wnt pathway. Thus, it may be suggested that ginger is a candidate to research in human RA treatment.
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spelling pubmed-101762302023-05-12 Ginger extract suppresses the activations of NF-κB and Wnt pathways and protects inflammatory arthritis Öz, Burak Orhan, Cemal Tuzcu, Mehmet Şahin, Nurhan Özercan, İbrahim Hanifi Öner, Pınar Demirel Serdar Koca, Süleyman Juturu, Vijaya Şahin, Kazım Eur J Rheumatol Original Articles OBJECTIVE: Rheumatoid arthritis (RA) is a disabling inflammatory disorder. Ginger is used for food and medicine to treat arthralgia, sprains, and muscle aches. Anti-inflammatory effects of ginger have been observed. The aim of our study was to detect the effects of ginger on experimentally induced inflammatory arthritis. METHODS: Female Wistar albino rats (n = 21) were randomly separated into three groups (control, arthritis, and arthritis + ginger). Arthritis was generated by an appropriate method using type 2 collagen and Freund's adjuvant (collagen-induced arthritis model). The ginger group was treated starting at the first collagen injection with ginger root extract for 32 days by oral gavage (50 mg/kg/daily). Interleukin (IL)-6, IL-17, tumor necrosis factor-α (TNF-α), sclerostin, dickkopf-related protein-1 (DKK-1), and obestatin serum levels were studied by enzyme-linked immunosorbent assay method. Tissue TNF-α, IL-17, cyclooxygenase-2 (COX-2), and nuclear factor kappa B (NF-κB) levels were detected using the Western blot method. RESULTS: Mean arthritis score and serum levels of TNF-α, IL-6, and IL-17 were significantly decreased in ginger group than in the arthritis group. Increased sclerostin serum level and decreased DKK-1 serum levels were detected in ginger group compared with arthritis group. The decreases of IL-17, TNF-α, COX-2, and NF-κB tissue levels were statistically significant in the ginger group compared with arthritis group. Histopathological evaluation of the ginger group showed a decrease in the inflammation score compared to arthritis group. CONCLUSION: It can be concluded that ginger has protective properties in the development of inflammatory arthritis. The antiarthritic acts of ginger are related to NF-κB activity and Wnt pathway. Thus, it may be suggested that ginger is a candidate to research in human RA treatment. Medical Research and Education Association 2022-01-21 /pmc/articles/PMC10176230/ /pubmed/35110180 http://dx.doi.org/10.5152/eujrheum.2020.20192 Text en © Copyright 2021 by Medical Research and Education Association https://creativecommons.org/licenses/by-nc/4.0/ Content of this journal is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. (https://creativecommons.org/licenses/by-nc/4.0/)
spellingShingle Original Articles
Öz, Burak
Orhan, Cemal
Tuzcu, Mehmet
Şahin, Nurhan
Özercan, İbrahim Hanifi
Öner, Pınar Demirel
Serdar Koca, Süleyman
Juturu, Vijaya
Şahin, Kazım
Ginger extract suppresses the activations of NF-κB and Wnt pathways and protects inflammatory arthritis
title Ginger extract suppresses the activations of NF-κB and Wnt pathways and protects inflammatory arthritis
title_full Ginger extract suppresses the activations of NF-κB and Wnt pathways and protects inflammatory arthritis
title_fullStr Ginger extract suppresses the activations of NF-κB and Wnt pathways and protects inflammatory arthritis
title_full_unstemmed Ginger extract suppresses the activations of NF-κB and Wnt pathways and protects inflammatory arthritis
title_short Ginger extract suppresses the activations of NF-κB and Wnt pathways and protects inflammatory arthritis
title_sort ginger extract suppresses the activations of nf-κb and wnt pathways and protects inflammatory arthritis
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10176230/
https://www.ncbi.nlm.nih.gov/pubmed/35110180
http://dx.doi.org/10.5152/eujrheum.2020.20192
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