Cargando…

Nano-antibiotic based on silver nanoparticles functionalized to the vancomycin–cysteamine complex for treating Staphylococcus aureus and Enterococcus faecalis

BACKGROUND: Bacterial resistance is defined as a microorganism’s capacity to develop mechanisms for resisting a determined antimicrobial. Gram-positive bacteria, such as Staphylococcus aureus (S. aureus) and Enterococcus faecalis (E. faecalis), are internationally recognized among the isolates with...

Descripción completa

Detalles Bibliográficos
Autores principales: Veriato, Thaís S., Fontoura, Inglid, Oliveira, Luciane D., Raniero, Leandro J., Castilho, Maiara L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10176295/
https://www.ncbi.nlm.nih.gov/pubmed/37171518
http://dx.doi.org/10.1007/s43440-023-00491-3
_version_ 1785040401643601920
author Veriato, Thaís S.
Fontoura, Inglid
Oliveira, Luciane D.
Raniero, Leandro J.
Castilho, Maiara L.
author_facet Veriato, Thaís S.
Fontoura, Inglid
Oliveira, Luciane D.
Raniero, Leandro J.
Castilho, Maiara L.
author_sort Veriato, Thaís S.
collection PubMed
description BACKGROUND: Bacterial resistance is defined as a microorganism’s capacity to develop mechanisms for resisting a determined antimicrobial. Gram-positive bacteria, such as Staphylococcus aureus (S. aureus) and Enterococcus faecalis (E. faecalis), are internationally recognized among the isolates with this resistance profile. In this context, the demand for new medicines has risen, and silver nanoparticles (AgNPs) have been highlighted, especially for their anti-bacterial effects. To develop a nano-antibiotic for treating these Gram-positive strains, we herein report synthesizing and characterizing a nano-antibiotic based on AgNPs functionalized with the complex vancomycin–cysteamine. METHODS: AgNPs were produced using the bottom-up methodology and functionalized with vancomycin modified by the carbodiimide chemistry, forming Ag@vancomycin. Susceptibility tests were performed using S. aureus and E. faecalis strains to assess the bacteriostatic and bactericidal potential of the developed nano-antibiotic. RESULTS: Fourier transform infrared spectroscopy measurements showed the efficacy of vancomycin chemical modification, and the characteristic bands of AgNPs functionalization with the antibiotic. The increase in the nano-antibiotic average hydrodynamic diameter observed by dynamic light scattering proved the presence of vancomycin at the surface of AgNPs. The data from the minimum inhibitory concentration and minimal bactericidal concentration assays tested on standard and clinical planktonic strains of S. aureus and E. faecalis presented excellent performance. CONCLUSION: The results indicate the promising development of a new nano-antibiotic in which the functionalization potentiates the bacteriostatic action of AgNPs and vancomycin with greater efficacy against Gram-positive strains. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s43440-023-00491-3.
format Online
Article
Text
id pubmed-10176295
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Springer International Publishing
record_format MEDLINE/PubMed
spelling pubmed-101762952023-05-14 Nano-antibiotic based on silver nanoparticles functionalized to the vancomycin–cysteamine complex for treating Staphylococcus aureus and Enterococcus faecalis Veriato, Thaís S. Fontoura, Inglid Oliveira, Luciane D. Raniero, Leandro J. Castilho, Maiara L. Pharmacol Rep Article BACKGROUND: Bacterial resistance is defined as a microorganism’s capacity to develop mechanisms for resisting a determined antimicrobial. Gram-positive bacteria, such as Staphylococcus aureus (S. aureus) and Enterococcus faecalis (E. faecalis), are internationally recognized among the isolates with this resistance profile. In this context, the demand for new medicines has risen, and silver nanoparticles (AgNPs) have been highlighted, especially for their anti-bacterial effects. To develop a nano-antibiotic for treating these Gram-positive strains, we herein report synthesizing and characterizing a nano-antibiotic based on AgNPs functionalized with the complex vancomycin–cysteamine. METHODS: AgNPs were produced using the bottom-up methodology and functionalized with vancomycin modified by the carbodiimide chemistry, forming Ag@vancomycin. Susceptibility tests were performed using S. aureus and E. faecalis strains to assess the bacteriostatic and bactericidal potential of the developed nano-antibiotic. RESULTS: Fourier transform infrared spectroscopy measurements showed the efficacy of vancomycin chemical modification, and the characteristic bands of AgNPs functionalization with the antibiotic. The increase in the nano-antibiotic average hydrodynamic diameter observed by dynamic light scattering proved the presence of vancomycin at the surface of AgNPs. The data from the minimum inhibitory concentration and minimal bactericidal concentration assays tested on standard and clinical planktonic strains of S. aureus and E. faecalis presented excellent performance. CONCLUSION: The results indicate the promising development of a new nano-antibiotic in which the functionalization potentiates the bacteriostatic action of AgNPs and vancomycin with greater efficacy against Gram-positive strains. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s43440-023-00491-3. Springer International Publishing 2023-05-12 /pmc/articles/PMC10176295/ /pubmed/37171518 http://dx.doi.org/10.1007/s43440-023-00491-3 Text en © The Author(s) under exclusive licence to Maj Institute of Pharmacology Polish Academy of Sciences 2023, Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Veriato, Thaís S.
Fontoura, Inglid
Oliveira, Luciane D.
Raniero, Leandro J.
Castilho, Maiara L.
Nano-antibiotic based on silver nanoparticles functionalized to the vancomycin–cysteamine complex for treating Staphylococcus aureus and Enterococcus faecalis
title Nano-antibiotic based on silver nanoparticles functionalized to the vancomycin–cysteamine complex for treating Staphylococcus aureus and Enterococcus faecalis
title_full Nano-antibiotic based on silver nanoparticles functionalized to the vancomycin–cysteamine complex for treating Staphylococcus aureus and Enterococcus faecalis
title_fullStr Nano-antibiotic based on silver nanoparticles functionalized to the vancomycin–cysteamine complex for treating Staphylococcus aureus and Enterococcus faecalis
title_full_unstemmed Nano-antibiotic based on silver nanoparticles functionalized to the vancomycin–cysteamine complex for treating Staphylococcus aureus and Enterococcus faecalis
title_short Nano-antibiotic based on silver nanoparticles functionalized to the vancomycin–cysteamine complex for treating Staphylococcus aureus and Enterococcus faecalis
title_sort nano-antibiotic based on silver nanoparticles functionalized to the vancomycin–cysteamine complex for treating staphylococcus aureus and enterococcus faecalis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10176295/
https://www.ncbi.nlm.nih.gov/pubmed/37171518
http://dx.doi.org/10.1007/s43440-023-00491-3
work_keys_str_mv AT veriatothaiss nanoantibioticbasedonsilvernanoparticlesfunctionalizedtothevancomycincysteaminecomplexfortreatingstaphylococcusaureusandenterococcusfaecalis
AT fontourainglid nanoantibioticbasedonsilvernanoparticlesfunctionalizedtothevancomycincysteaminecomplexfortreatingstaphylococcusaureusandenterococcusfaecalis
AT oliveiralucianed nanoantibioticbasedonsilvernanoparticlesfunctionalizedtothevancomycincysteaminecomplexfortreatingstaphylococcusaureusandenterococcusfaecalis
AT ranieroleandroj nanoantibioticbasedonsilvernanoparticlesfunctionalizedtothevancomycincysteaminecomplexfortreatingstaphylococcusaureusandenterococcusfaecalis
AT castilhomaiaral nanoantibioticbasedonsilvernanoparticlesfunctionalizedtothevancomycincysteaminecomplexfortreatingstaphylococcusaureusandenterococcusfaecalis