A Multiparametric and High-Throughput Platform for Host–Virus Binding Screens

[Image: see text] Speed is key during infectious disease outbreaks. It is essential, for example, to identify critical host binding factors to pathogens as fast as possible. The complexity of host plasma membrane is often a limiting factor hindering fast and accurate determination of host binding fa...

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Autores principales: Schlegel, Jan, Porebski, Bartlomiej, Andronico, Luca, Hanke, Leo, Edwards, Steven, Brismar, Hjalmar, Murrell, Ben, McInerney, Gerald M., Fernandez-Capetillo, Oscar, Sezgin, Erdinc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10176574/
https://www.ncbi.nlm.nih.gov/pubmed/36892970
http://dx.doi.org/10.1021/acs.nanolett.2c04884
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author Schlegel, Jan
Porebski, Bartlomiej
Andronico, Luca
Hanke, Leo
Edwards, Steven
Brismar, Hjalmar
Murrell, Ben
McInerney, Gerald M.
Fernandez-Capetillo, Oscar
Sezgin, Erdinc
author_facet Schlegel, Jan
Porebski, Bartlomiej
Andronico, Luca
Hanke, Leo
Edwards, Steven
Brismar, Hjalmar
Murrell, Ben
McInerney, Gerald M.
Fernandez-Capetillo, Oscar
Sezgin, Erdinc
author_sort Schlegel, Jan
collection PubMed
description [Image: see text] Speed is key during infectious disease outbreaks. It is essential, for example, to identify critical host binding factors to pathogens as fast as possible. The complexity of host plasma membrane is often a limiting factor hindering fast and accurate determination of host binding factors as well as high-throughput screening for neutralizing antimicrobial drug targets. Here, we describe a multiparametric and high-throughput platform tackling this bottleneck and enabling fast screens for host binding factors as well as new antiviral drug targets. The sensitivity and robustness of our platform were validated by blocking SARS-CoV-2 particles with nanobodies and IgGs from human serum samples.
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spelling pubmed-101765742023-05-13 A Multiparametric and High-Throughput Platform for Host–Virus Binding Screens Schlegel, Jan Porebski, Bartlomiej Andronico, Luca Hanke, Leo Edwards, Steven Brismar, Hjalmar Murrell, Ben McInerney, Gerald M. Fernandez-Capetillo, Oscar Sezgin, Erdinc Nano Lett [Image: see text] Speed is key during infectious disease outbreaks. It is essential, for example, to identify critical host binding factors to pathogens as fast as possible. The complexity of host plasma membrane is often a limiting factor hindering fast and accurate determination of host binding factors as well as high-throughput screening for neutralizing antimicrobial drug targets. Here, we describe a multiparametric and high-throughput platform tackling this bottleneck and enabling fast screens for host binding factors as well as new antiviral drug targets. The sensitivity and robustness of our platform were validated by blocking SARS-CoV-2 particles with nanobodies and IgGs from human serum samples. American Chemical Society 2023-03-09 /pmc/articles/PMC10176574/ /pubmed/36892970 http://dx.doi.org/10.1021/acs.nanolett.2c04884 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Schlegel, Jan
Porebski, Bartlomiej
Andronico, Luca
Hanke, Leo
Edwards, Steven
Brismar, Hjalmar
Murrell, Ben
McInerney, Gerald M.
Fernandez-Capetillo, Oscar
Sezgin, Erdinc
A Multiparametric and High-Throughput Platform for Host–Virus Binding Screens
title A Multiparametric and High-Throughput Platform for Host–Virus Binding Screens
title_full A Multiparametric and High-Throughput Platform for Host–Virus Binding Screens
title_fullStr A Multiparametric and High-Throughput Platform for Host–Virus Binding Screens
title_full_unstemmed A Multiparametric and High-Throughput Platform for Host–Virus Binding Screens
title_short A Multiparametric and High-Throughput Platform for Host–Virus Binding Screens
title_sort multiparametric and high-throughput platform for host–virus binding screens
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10176574/
https://www.ncbi.nlm.nih.gov/pubmed/36892970
http://dx.doi.org/10.1021/acs.nanolett.2c04884
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