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AGO2a but not AGO2b mediates antiviral defense against infection of wild-type cucumber mosaic virus in tomato
Evolutionarily conserved antiviral RNA interference (RNAi) mediates a primary antiviral innate immunity preventing infection of broad-spectrum viruses in plants. However, the detailed mechanism in plants is still largely unknown, especially in important agricultural crops, including tomato. Varietie...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10177002/ https://www.ncbi.nlm.nih.gov/pubmed/37188058 http://dx.doi.org/10.1093/hr/uhad043 |
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author | Zhao, Liling Chen, Yingfang Xiao, Xingming Gao, Haiying Cao, Jiamin Zhang, Zhongkai Guo, Zhongxin |
author_facet | Zhao, Liling Chen, Yingfang Xiao, Xingming Gao, Haiying Cao, Jiamin Zhang, Zhongkai Guo, Zhongxin |
author_sort | Zhao, Liling |
collection | PubMed |
description | Evolutionarily conserved antiviral RNA interference (RNAi) mediates a primary antiviral innate immunity preventing infection of broad-spectrum viruses in plants. However, the detailed mechanism in plants is still largely unknown, especially in important agricultural crops, including tomato. Varieties of pathogenic viruses evolve to possess viral suppressors of RNA silencing (VSRs) to suppress antiviral RNAi in the host. Due to the prevalence of VSRs, it is still unknown whether antiviral RNAi truly functions to prevent invasion by natural wild-type viruses in plants and animals. In this research, for the first time we applied CRISPR-Cas9 to generate ago2a, ago2b, or ago2ab mutants for two differentiated Solanum lycopersicum AGO2s, key effectors in antiviral RNAi. We found that AGO2a but not AGO2b was significantly induced to inhibit the propagation of not only VSR-deficient Cucumber mosaic virus (CMV) but also wild-type CMV-Fny in tomato; however, neither AGO2a nor AGO2b regulated disease induction after infection with either virus. Our findings firstly reveal a prominent role of AGO2a in antiviral RNAi innate immunity in tomato and demonstrate that antiviral RNAi evolves to defend against infection of natural wild-type CMV-Fny in tomato. However, AGO2a-mediated antiviral RNAi does not play major roles in promoting tolerance of tomato plants to CMV infection for maintaining health. |
format | Online Article Text |
id | pubmed-10177002 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-101770022023-05-13 AGO2a but not AGO2b mediates antiviral defense against infection of wild-type cucumber mosaic virus in tomato Zhao, Liling Chen, Yingfang Xiao, Xingming Gao, Haiying Cao, Jiamin Zhang, Zhongkai Guo, Zhongxin Hortic Res Article Evolutionarily conserved antiviral RNA interference (RNAi) mediates a primary antiviral innate immunity preventing infection of broad-spectrum viruses in plants. However, the detailed mechanism in plants is still largely unknown, especially in important agricultural crops, including tomato. Varieties of pathogenic viruses evolve to possess viral suppressors of RNA silencing (VSRs) to suppress antiviral RNAi in the host. Due to the prevalence of VSRs, it is still unknown whether antiviral RNAi truly functions to prevent invasion by natural wild-type viruses in plants and animals. In this research, for the first time we applied CRISPR-Cas9 to generate ago2a, ago2b, or ago2ab mutants for two differentiated Solanum lycopersicum AGO2s, key effectors in antiviral RNAi. We found that AGO2a but not AGO2b was significantly induced to inhibit the propagation of not only VSR-deficient Cucumber mosaic virus (CMV) but also wild-type CMV-Fny in tomato; however, neither AGO2a nor AGO2b regulated disease induction after infection with either virus. Our findings firstly reveal a prominent role of AGO2a in antiviral RNAi innate immunity in tomato and demonstrate that antiviral RNAi evolves to defend against infection of natural wild-type CMV-Fny in tomato. However, AGO2a-mediated antiviral RNAi does not play major roles in promoting tolerance of tomato plants to CMV infection for maintaining health. Oxford University Press 2023-03-13 /pmc/articles/PMC10177002/ /pubmed/37188058 http://dx.doi.org/10.1093/hr/uhad043 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of Nanjing Agricultural University. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Zhao, Liling Chen, Yingfang Xiao, Xingming Gao, Haiying Cao, Jiamin Zhang, Zhongkai Guo, Zhongxin AGO2a but not AGO2b mediates antiviral defense against infection of wild-type cucumber mosaic virus in tomato |
title | AGO2a but not AGO2b mediates antiviral defense against infection of wild-type cucumber mosaic virus in tomato |
title_full | AGO2a but not AGO2b mediates antiviral defense against infection of wild-type cucumber mosaic virus in tomato |
title_fullStr | AGO2a but not AGO2b mediates antiviral defense against infection of wild-type cucumber mosaic virus in tomato |
title_full_unstemmed | AGO2a but not AGO2b mediates antiviral defense against infection of wild-type cucumber mosaic virus in tomato |
title_short | AGO2a but not AGO2b mediates antiviral defense against infection of wild-type cucumber mosaic virus in tomato |
title_sort | ago2a but not ago2b mediates antiviral defense against infection of wild-type cucumber mosaic virus in tomato |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10177002/ https://www.ncbi.nlm.nih.gov/pubmed/37188058 http://dx.doi.org/10.1093/hr/uhad043 |
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