Suspension TRAPping Filter (sTRAP) Sample Preparation for Quantitative Proteomics in the Low µg Input Range Using a Plasmid DNA Micro-Spin Column: Analysis of the Hippocampus from the 5xFAD Alzheimer’s Disease Mouse Model

Suspension TRAPping filter (sTRAP) is an attractive sample preparation method for proteomics studies. The sTRAP protocol uses 5% SDS that maximizes protein solubilization. Proteins are trapped on a borosilicate glass membrane filter, where SDS is subsequently removed from the filter. After trypsin d...

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Detalles Bibliográficos
Autores principales: Thanou, Evangelia, Koopmans, Frank, Pita-Illobre, Débora, Klaassen, Remco V., Özer, Berna, Charalampopoulos, Ioannis, Smit, August B., Li, Ka Wan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10177283/
https://www.ncbi.nlm.nih.gov/pubmed/37174641
http://dx.doi.org/10.3390/cells12091242

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