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Kompetitive Allele Specific PCR Genotyping of 89 SNPs in Romanian Spotted and Romanian Brown Cattle Breeds and Their Association with Clinical Mastitis

SIMPLE SUMMARY: In order to improve animals’ resistance to mastitis in subsequent generations and to reduce disease prevalence, one feasible approach could be the genomic selection of cows with the most potentially protective immune responses. In this study, 298 cattle were genotyped for 89 single n...

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Autores principales: Ilie, Daniela Elena, Gavojdian, Dinu, Kusza, Szilvia, Neamț, Radu Ionel, Mizeranschi, Alexandru Eugeniu, Mihali, Ciprian Valentin, Cziszter, Ludovic Toma
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10177413/
https://www.ncbi.nlm.nih.gov/pubmed/37174521
http://dx.doi.org/10.3390/ani13091484
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author Ilie, Daniela Elena
Gavojdian, Dinu
Kusza, Szilvia
Neamț, Radu Ionel
Mizeranschi, Alexandru Eugeniu
Mihali, Ciprian Valentin
Cziszter, Ludovic Toma
author_facet Ilie, Daniela Elena
Gavojdian, Dinu
Kusza, Szilvia
Neamț, Radu Ionel
Mizeranschi, Alexandru Eugeniu
Mihali, Ciprian Valentin
Cziszter, Ludovic Toma
author_sort Ilie, Daniela Elena
collection PubMed
description SIMPLE SUMMARY: In order to improve animals’ resistance to mastitis in subsequent generations and to reduce disease prevalence, one feasible approach could be the genomic selection of cows with the most potentially protective immune responses. In this study, 298 cattle were genotyped for 89 single nucleotide polymorphisms through the Kompetitive Allele Specific PCR. Our findings reveal a significant association of a molecular marker in the BOLA-DRB3 gene with mastitis prevalence in the investigated breeds. This supports previous studies that the BOLA-DRB3 gene represents an important locus affecting resistance to mastitis in cattle. These results contribute to an increase in knowledge and available data regarding the genetic variability of SNPs for udder health in cattle. ABSTRACT: Mastitis is the most common production disease in the dairy sector worldwide, its incidence being associated with both cows’ exposure to bacteria and the cows’ genetic make-up for resistance to pathogens. The objective of our study was to analyse 89 missense SNPs belonging to six genes (CXCR2, CXCL8, TLR4, BRCA1, LTF, BOLA-DRB3), which were found to be associated with genetic resistance or susceptibility to mastitis. A total of 298 cattle (250 Romanian Spotted and 48 Romanian Brown) were genotyped by Kompetitive Allele Specific PCR (KASP) and a chi-squared test was used for genetic association studies with clinical mastitis. A total of 35 SNPs (39.3%) among the selected 89 SNPs were successfully genotyped, of which 31 markers were monomorphic. The polymorphic markers were found in two genes: TLR4 (rs460053411) and BOLA-DRB3 (rs42309897, rs208816121, rs110124025). The polymorphic SNPs with MAF > 5% and call rates > 95% were used for the association study. The results showed that rs110124025 in the BOLA-DRB3 gene was significantly associated with mastitis prevalence (p ≤ 0.05) in both investigated breeds. Current results show that the SNP rs110124025 in the BOLA-DRB3 gene can be used as a candidate genetic marker in selection for mastitis resistance in Romanian dairy cattle.
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spelling pubmed-101774132023-05-13 Kompetitive Allele Specific PCR Genotyping of 89 SNPs in Romanian Spotted and Romanian Brown Cattle Breeds and Their Association with Clinical Mastitis Ilie, Daniela Elena Gavojdian, Dinu Kusza, Szilvia Neamț, Radu Ionel Mizeranschi, Alexandru Eugeniu Mihali, Ciprian Valentin Cziszter, Ludovic Toma Animals (Basel) Article SIMPLE SUMMARY: In order to improve animals’ resistance to mastitis in subsequent generations and to reduce disease prevalence, one feasible approach could be the genomic selection of cows with the most potentially protective immune responses. In this study, 298 cattle were genotyped for 89 single nucleotide polymorphisms through the Kompetitive Allele Specific PCR. Our findings reveal a significant association of a molecular marker in the BOLA-DRB3 gene with mastitis prevalence in the investigated breeds. This supports previous studies that the BOLA-DRB3 gene represents an important locus affecting resistance to mastitis in cattle. These results contribute to an increase in knowledge and available data regarding the genetic variability of SNPs for udder health in cattle. ABSTRACT: Mastitis is the most common production disease in the dairy sector worldwide, its incidence being associated with both cows’ exposure to bacteria and the cows’ genetic make-up for resistance to pathogens. The objective of our study was to analyse 89 missense SNPs belonging to six genes (CXCR2, CXCL8, TLR4, BRCA1, LTF, BOLA-DRB3), which were found to be associated with genetic resistance or susceptibility to mastitis. A total of 298 cattle (250 Romanian Spotted and 48 Romanian Brown) were genotyped by Kompetitive Allele Specific PCR (KASP) and a chi-squared test was used for genetic association studies with clinical mastitis. A total of 35 SNPs (39.3%) among the selected 89 SNPs were successfully genotyped, of which 31 markers were monomorphic. The polymorphic markers were found in two genes: TLR4 (rs460053411) and BOLA-DRB3 (rs42309897, rs208816121, rs110124025). The polymorphic SNPs with MAF > 5% and call rates > 95% were used for the association study. The results showed that rs110124025 in the BOLA-DRB3 gene was significantly associated with mastitis prevalence (p ≤ 0.05) in both investigated breeds. Current results show that the SNP rs110124025 in the BOLA-DRB3 gene can be used as a candidate genetic marker in selection for mastitis resistance in Romanian dairy cattle. MDPI 2023-04-27 /pmc/articles/PMC10177413/ /pubmed/37174521 http://dx.doi.org/10.3390/ani13091484 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ilie, Daniela Elena
Gavojdian, Dinu
Kusza, Szilvia
Neamț, Radu Ionel
Mizeranschi, Alexandru Eugeniu
Mihali, Ciprian Valentin
Cziszter, Ludovic Toma
Kompetitive Allele Specific PCR Genotyping of 89 SNPs in Romanian Spotted and Romanian Brown Cattle Breeds and Their Association with Clinical Mastitis
title Kompetitive Allele Specific PCR Genotyping of 89 SNPs in Romanian Spotted and Romanian Brown Cattle Breeds and Their Association with Clinical Mastitis
title_full Kompetitive Allele Specific PCR Genotyping of 89 SNPs in Romanian Spotted and Romanian Brown Cattle Breeds and Their Association with Clinical Mastitis
title_fullStr Kompetitive Allele Specific PCR Genotyping of 89 SNPs in Romanian Spotted and Romanian Brown Cattle Breeds and Their Association with Clinical Mastitis
title_full_unstemmed Kompetitive Allele Specific PCR Genotyping of 89 SNPs in Romanian Spotted and Romanian Brown Cattle Breeds and Their Association with Clinical Mastitis
title_short Kompetitive Allele Specific PCR Genotyping of 89 SNPs in Romanian Spotted and Romanian Brown Cattle Breeds and Their Association with Clinical Mastitis
title_sort kompetitive allele specific pcr genotyping of 89 snps in romanian spotted and romanian brown cattle breeds and their association with clinical mastitis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10177413/
https://www.ncbi.nlm.nih.gov/pubmed/37174521
http://dx.doi.org/10.3390/ani13091484
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