Cargando…
Comprehensive Brain Tumour Characterisation with VERDICT-MRI: Evaluation of Cellular and Vascular Measures Validated by Histology
SIMPLE SUMMARY: VERDICT (Vascular, Extracellular, and Restricted DIffusion for Cytometry in Tumours) is a diffusion MRI framework for the characterisation of different components of tumours, which has shown diagnostic utility for body cancer. The aim of this study was to extend the VERDICT framework...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10177485/ https://www.ncbi.nlm.nih.gov/pubmed/37173965 http://dx.doi.org/10.3390/cancers15092490 |
_version_ | 1785040649373876224 |
---|---|
author | Figini, Matteo Castellano, Antonella Bailo, Michele Callea, Marcella Cadioli, Marcello Bouyagoub, Samira Palombo, Marco Pieri, Valentina Mortini, Pietro Falini, Andrea Alexander, Daniel C. Cercignani, Mara Panagiotaki, Eleftheria |
author_facet | Figini, Matteo Castellano, Antonella Bailo, Michele Callea, Marcella Cadioli, Marcello Bouyagoub, Samira Palombo, Marco Pieri, Valentina Mortini, Pietro Falini, Andrea Alexander, Daniel C. Cercignani, Mara Panagiotaki, Eleftheria |
author_sort | Figini, Matteo |
collection | PubMed |
description | SIMPLE SUMMARY: VERDICT (Vascular, Extracellular, and Restricted DIffusion for Cytometry in Tumours) is a diffusion MRI framework for the characterisation of different components of tumours, which has shown diagnostic utility for body cancer. The aim of this study was to extend the VERDICT framework to comprehensively characterise brain tumours, which is challenging due to the complexity of brain tissues. The resulting biomarkers showed agreement with histology and followed the expected trends when comparing different tumour types and sub-regions. These preliminary results hold promise for the non-invasive characterisation of brain tumours by VERDICT-MRI, which would be an important tool for diagnosis and monitoring of treatment effects. ABSTRACT: The aim of this work was to extend the VERDICT-MRI framework for modelling brain tumours, enabling comprehensive characterisation of both intra- and peritumoural areas with a particular focus on cellular and vascular features. Diffusion MRI data were acquired with multiple b-values (ranging from 50 to 3500 s/mm(2)), diffusion times, and echo times in 21 patients with brain tumours of different types and with a wide range of cellular and vascular features. We fitted a selection of diffusion models that resulted from the combination of different types of intracellular, extracellular, and vascular compartments to the signal. We compared the models using criteria for parsimony while aiming at good characterisation of all of the key histological brain tumour components. Finally, we evaluated the parameters of the best-performing model in the differentiation of tumour histotypes, using ADC (Apparent Diffusion Coefficient) as a clinical standard reference, and compared them to histopathology and relevant perfusion MRI metrics. The best-performing model for VERDICT in brain tumours was a three-compartment model accounting for anisotropically hindered and isotropically restricted diffusion and isotropic pseudo-diffusion. VERDICT metrics were compatible with the histological appearance of low-grade gliomas and metastases and reflected differences found by histopathology between multiple biopsy samples within tumours. The comparison between histotypes showed that both the intracellular and vascular fractions tended to be higher in tumours with high cellularity (glioblastoma and metastasis), and quantitative analysis showed a trend toward higher values of the intracellular fraction (fic) within the tumour core with increasing glioma grade. We also observed a trend towards a higher free water fraction in vasogenic oedemas around metastases compared to infiltrative oedemas around glioblastomas and WHO 3 gliomas as well as the periphery of low-grade gliomas. In conclusion, we developed and evaluated a multi-compartment diffusion MRI model for brain tumours based on the VERDICT framework, which showed agreement between non-invasive microstructural estimates and histology and encouraging trends for the differentiation of tumour types and sub-regions. |
format | Online Article Text |
id | pubmed-10177485 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101774852023-05-13 Comprehensive Brain Tumour Characterisation with VERDICT-MRI: Evaluation of Cellular and Vascular Measures Validated by Histology Figini, Matteo Castellano, Antonella Bailo, Michele Callea, Marcella Cadioli, Marcello Bouyagoub, Samira Palombo, Marco Pieri, Valentina Mortini, Pietro Falini, Andrea Alexander, Daniel C. Cercignani, Mara Panagiotaki, Eleftheria Cancers (Basel) Article SIMPLE SUMMARY: VERDICT (Vascular, Extracellular, and Restricted DIffusion for Cytometry in Tumours) is a diffusion MRI framework for the characterisation of different components of tumours, which has shown diagnostic utility for body cancer. The aim of this study was to extend the VERDICT framework to comprehensively characterise brain tumours, which is challenging due to the complexity of brain tissues. The resulting biomarkers showed agreement with histology and followed the expected trends when comparing different tumour types and sub-regions. These preliminary results hold promise for the non-invasive characterisation of brain tumours by VERDICT-MRI, which would be an important tool for diagnosis and monitoring of treatment effects. ABSTRACT: The aim of this work was to extend the VERDICT-MRI framework for modelling brain tumours, enabling comprehensive characterisation of both intra- and peritumoural areas with a particular focus on cellular and vascular features. Diffusion MRI data were acquired with multiple b-values (ranging from 50 to 3500 s/mm(2)), diffusion times, and echo times in 21 patients with brain tumours of different types and with a wide range of cellular and vascular features. We fitted a selection of diffusion models that resulted from the combination of different types of intracellular, extracellular, and vascular compartments to the signal. We compared the models using criteria for parsimony while aiming at good characterisation of all of the key histological brain tumour components. Finally, we evaluated the parameters of the best-performing model in the differentiation of tumour histotypes, using ADC (Apparent Diffusion Coefficient) as a clinical standard reference, and compared them to histopathology and relevant perfusion MRI metrics. The best-performing model for VERDICT in brain tumours was a three-compartment model accounting for anisotropically hindered and isotropically restricted diffusion and isotropic pseudo-diffusion. VERDICT metrics were compatible with the histological appearance of low-grade gliomas and metastases and reflected differences found by histopathology between multiple biopsy samples within tumours. The comparison between histotypes showed that both the intracellular and vascular fractions tended to be higher in tumours with high cellularity (glioblastoma and metastasis), and quantitative analysis showed a trend toward higher values of the intracellular fraction (fic) within the tumour core with increasing glioma grade. We also observed a trend towards a higher free water fraction in vasogenic oedemas around metastases compared to infiltrative oedemas around glioblastomas and WHO 3 gliomas as well as the periphery of low-grade gliomas. In conclusion, we developed and evaluated a multi-compartment diffusion MRI model for brain tumours based on the VERDICT framework, which showed agreement between non-invasive microstructural estimates and histology and encouraging trends for the differentiation of tumour types and sub-regions. MDPI 2023-04-27 /pmc/articles/PMC10177485/ /pubmed/37173965 http://dx.doi.org/10.3390/cancers15092490 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Figini, Matteo Castellano, Antonella Bailo, Michele Callea, Marcella Cadioli, Marcello Bouyagoub, Samira Palombo, Marco Pieri, Valentina Mortini, Pietro Falini, Andrea Alexander, Daniel C. Cercignani, Mara Panagiotaki, Eleftheria Comprehensive Brain Tumour Characterisation with VERDICT-MRI: Evaluation of Cellular and Vascular Measures Validated by Histology |
title | Comprehensive Brain Tumour Characterisation with VERDICT-MRI: Evaluation of Cellular and Vascular Measures Validated by Histology |
title_full | Comprehensive Brain Tumour Characterisation with VERDICT-MRI: Evaluation of Cellular and Vascular Measures Validated by Histology |
title_fullStr | Comprehensive Brain Tumour Characterisation with VERDICT-MRI: Evaluation of Cellular and Vascular Measures Validated by Histology |
title_full_unstemmed | Comprehensive Brain Tumour Characterisation with VERDICT-MRI: Evaluation of Cellular and Vascular Measures Validated by Histology |
title_short | Comprehensive Brain Tumour Characterisation with VERDICT-MRI: Evaluation of Cellular and Vascular Measures Validated by Histology |
title_sort | comprehensive brain tumour characterisation with verdict-mri: evaluation of cellular and vascular measures validated by histology |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10177485/ https://www.ncbi.nlm.nih.gov/pubmed/37173965 http://dx.doi.org/10.3390/cancers15092490 |
work_keys_str_mv | AT figinimatteo comprehensivebraintumourcharacterisationwithverdictmrievaluationofcellularandvascularmeasuresvalidatedbyhistology AT castellanoantonella comprehensivebraintumourcharacterisationwithverdictmrievaluationofcellularandvascularmeasuresvalidatedbyhistology AT bailomichele comprehensivebraintumourcharacterisationwithverdictmrievaluationofcellularandvascularmeasuresvalidatedbyhistology AT calleamarcella comprehensivebraintumourcharacterisationwithverdictmrievaluationofcellularandvascularmeasuresvalidatedbyhistology AT cadiolimarcello comprehensivebraintumourcharacterisationwithverdictmrievaluationofcellularandvascularmeasuresvalidatedbyhistology AT bouyagoubsamira comprehensivebraintumourcharacterisationwithverdictmrievaluationofcellularandvascularmeasuresvalidatedbyhistology AT palombomarco comprehensivebraintumourcharacterisationwithverdictmrievaluationofcellularandvascularmeasuresvalidatedbyhistology AT pierivalentina comprehensivebraintumourcharacterisationwithverdictmrievaluationofcellularandvascularmeasuresvalidatedbyhistology AT mortinipietro comprehensivebraintumourcharacterisationwithverdictmrievaluationofcellularandvascularmeasuresvalidatedbyhistology AT faliniandrea comprehensivebraintumourcharacterisationwithverdictmrievaluationofcellularandvascularmeasuresvalidatedbyhistology AT alexanderdanielc comprehensivebraintumourcharacterisationwithverdictmrievaluationofcellularandvascularmeasuresvalidatedbyhistology AT cercignanimara comprehensivebraintumourcharacterisationwithverdictmrievaluationofcellularandvascularmeasuresvalidatedbyhistology AT panagiotakieleftheria comprehensivebraintumourcharacterisationwithverdictmrievaluationofcellularandvascularmeasuresvalidatedbyhistology |