Targeted knock-ins with pseudovirus for the stable expression of large transgenes

We engineered integrase-deficient lentiviruses to act as vectors for the delivery of large gene knock-ins via homology-directed repair. This technology enables the non-cytotoxic, targeted insertion of difficult-to-express transgenes into genomic loci that are essential to cell survival, thereby over...

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Detalles Bibliográficos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Research Briefing
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10177726/
https://www.ncbi.nlm.nih.gov/pubmed/37173433
http://dx.doi.org/10.1038/s41551-023-01044-y
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collection PubMed
description We engineered integrase-deficient lentiviruses to act as vectors for the delivery of large gene knock-ins via homology-directed repair. This technology enables the non-cytotoxic, targeted insertion of difficult-to-express transgenes into genomic loci that are essential to cell survival, thereby overcoming the gene silencing that otherwise limits primary immune cell engineering.
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spelling pubmed-101777262023-05-14 Targeted knock-ins with pseudovirus for the stable expression of large transgenes Nat Biomed Eng Research Briefing We engineered integrase-deficient lentiviruses to act as vectors for the delivery of large gene knock-ins via homology-directed repair. This technology enables the non-cytotoxic, targeted insertion of difficult-to-express transgenes into genomic loci that are essential to cell survival, thereby overcoming the gene silencing that otherwise limits primary immune cell engineering. Nature Publishing Group UK 2023-05-12 2023 /pmc/articles/PMC10177726/ /pubmed/37173433 http://dx.doi.org/10.1038/s41551-023-01044-y Text en © The Author(s), under exclusive licence to Springer Nature Limited 2023 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Research Briefing
Targeted knock-ins with pseudovirus for the stable expression of large transgenes
title Targeted knock-ins with pseudovirus for the stable expression of large transgenes
title_full Targeted knock-ins with pseudovirus for the stable expression of large transgenes
title_fullStr Targeted knock-ins with pseudovirus for the stable expression of large transgenes
title_full_unstemmed Targeted knock-ins with pseudovirus for the stable expression of large transgenes
title_short Targeted knock-ins with pseudovirus for the stable expression of large transgenes
title_sort targeted knock-ins with pseudovirus for the stable expression of large transgenes
topic Research Briefing
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10177726/
https://www.ncbi.nlm.nih.gov/pubmed/37173433
http://dx.doi.org/10.1038/s41551-023-01044-y

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