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Targeted knock-ins with pseudovirus for the stable expression of large transgenes
We engineered integrase-deficient lentiviruses to act as vectors for the delivery of large gene knock-ins via homology-directed repair. This technology enables the non-cytotoxic, targeted insertion of difficult-to-express transgenes into genomic loci that are essential to cell survival, thereby over...
| Formato: | Online Artículo Texto |
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| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2023
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10177726/ https://www.ncbi.nlm.nih.gov/pubmed/37173433 http://dx.doi.org/10.1038/s41551-023-01044-y |