Cargando…
Multiplexing Biosensor for the Detection of Extracellular Vesicles as Biomarkers of Tissue Damage and Recovery after Ischemic Stroke
The inflammatory, reparative and regenerative mechanisms activated in ischemic stroke patients immediately after the event cooperate in the response to injury, in the restoration of functions and in brain remodeling even weeks after the event and can be sustained by the rehabilitation treatment. Non...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10177901/ https://www.ncbi.nlm.nih.gov/pubmed/37175644 http://dx.doi.org/10.3390/ijms24097937 |
_version_ | 1785040733172924416 |
---|---|
author | Picciolini, Silvia Mangolini, Valentina Rodà, Francesca Montesano, Angelo Arnaboldi, Francesca Liuzzi, Piergiuseppe Mannini, Andrea Bedoni, Marzia Gualerzi, Alice |
author_facet | Picciolini, Silvia Mangolini, Valentina Rodà, Francesca Montesano, Angelo Arnaboldi, Francesca Liuzzi, Piergiuseppe Mannini, Andrea Bedoni, Marzia Gualerzi, Alice |
author_sort | Picciolini, Silvia |
collection | PubMed |
description | The inflammatory, reparative and regenerative mechanisms activated in ischemic stroke patients immediately after the event cooperate in the response to injury, in the restoration of functions and in brain remodeling even weeks after the event and can be sustained by the rehabilitation treatment. Nonetheless, patients’ response to treatments is difficult to predict because of the lack of specific measurable markers of recovery, which could be complementary to clinical scales in the evaluation of patients. Considering that Extracellular Vesicles (EVs) are carriers of multiple molecules involved in the response to stroke injury, in the present study, we have identified a panel of EV-associated molecules that (i) confirm the crucial involvement of EVs in the processes that follow ischemic stroke, (ii) could possibly profile ischemic stroke patients at the beginning of the rehabilitation program, (iii) could be used in predicting patients’ response to treatment. By means of a multiplexing Surface Plasmon Resonance imaging biosensor, subacute ischemic stroke patients were proven to have increased expression of vascular endothelial growth factor receptor 2 (VEGFR2) and translocator protein (TSPO) on the surface of small EVs in blood. Besides, microglia EVs and endothelial EVs were shown to be significantly involved in the intercellular communications that occur more than 10 days after ischemic stroke, thus being potential tools for the profiling of patients in the subacute phase after ischemic stroke and in the prediction of their recovery. |
format | Online Article Text |
id | pubmed-10177901 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101779012023-05-13 Multiplexing Biosensor for the Detection of Extracellular Vesicles as Biomarkers of Tissue Damage and Recovery after Ischemic Stroke Picciolini, Silvia Mangolini, Valentina Rodà, Francesca Montesano, Angelo Arnaboldi, Francesca Liuzzi, Piergiuseppe Mannini, Andrea Bedoni, Marzia Gualerzi, Alice Int J Mol Sci Article The inflammatory, reparative and regenerative mechanisms activated in ischemic stroke patients immediately after the event cooperate in the response to injury, in the restoration of functions and in brain remodeling even weeks after the event and can be sustained by the rehabilitation treatment. Nonetheless, patients’ response to treatments is difficult to predict because of the lack of specific measurable markers of recovery, which could be complementary to clinical scales in the evaluation of patients. Considering that Extracellular Vesicles (EVs) are carriers of multiple molecules involved in the response to stroke injury, in the present study, we have identified a panel of EV-associated molecules that (i) confirm the crucial involvement of EVs in the processes that follow ischemic stroke, (ii) could possibly profile ischemic stroke patients at the beginning of the rehabilitation program, (iii) could be used in predicting patients’ response to treatment. By means of a multiplexing Surface Plasmon Resonance imaging biosensor, subacute ischemic stroke patients were proven to have increased expression of vascular endothelial growth factor receptor 2 (VEGFR2) and translocator protein (TSPO) on the surface of small EVs in blood. Besides, microglia EVs and endothelial EVs were shown to be significantly involved in the intercellular communications that occur more than 10 days after ischemic stroke, thus being potential tools for the profiling of patients in the subacute phase after ischemic stroke and in the prediction of their recovery. MDPI 2023-04-27 /pmc/articles/PMC10177901/ /pubmed/37175644 http://dx.doi.org/10.3390/ijms24097937 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Picciolini, Silvia Mangolini, Valentina Rodà, Francesca Montesano, Angelo Arnaboldi, Francesca Liuzzi, Piergiuseppe Mannini, Andrea Bedoni, Marzia Gualerzi, Alice Multiplexing Biosensor for the Detection of Extracellular Vesicles as Biomarkers of Tissue Damage and Recovery after Ischemic Stroke |
title | Multiplexing Biosensor for the Detection of Extracellular Vesicles as Biomarkers of Tissue Damage and Recovery after Ischemic Stroke |
title_full | Multiplexing Biosensor for the Detection of Extracellular Vesicles as Biomarkers of Tissue Damage and Recovery after Ischemic Stroke |
title_fullStr | Multiplexing Biosensor for the Detection of Extracellular Vesicles as Biomarkers of Tissue Damage and Recovery after Ischemic Stroke |
title_full_unstemmed | Multiplexing Biosensor for the Detection of Extracellular Vesicles as Biomarkers of Tissue Damage and Recovery after Ischemic Stroke |
title_short | Multiplexing Biosensor for the Detection of Extracellular Vesicles as Biomarkers of Tissue Damage and Recovery after Ischemic Stroke |
title_sort | multiplexing biosensor for the detection of extracellular vesicles as biomarkers of tissue damage and recovery after ischemic stroke |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10177901/ https://www.ncbi.nlm.nih.gov/pubmed/37175644 http://dx.doi.org/10.3390/ijms24097937 |
work_keys_str_mv | AT picciolinisilvia multiplexingbiosensorforthedetectionofextracellularvesiclesasbiomarkersoftissuedamageandrecoveryafterischemicstroke AT mangolinivalentina multiplexingbiosensorforthedetectionofextracellularvesiclesasbiomarkersoftissuedamageandrecoveryafterischemicstroke AT rodafrancesca multiplexingbiosensorforthedetectionofextracellularvesiclesasbiomarkersoftissuedamageandrecoveryafterischemicstroke AT montesanoangelo multiplexingbiosensorforthedetectionofextracellularvesiclesasbiomarkersoftissuedamageandrecoveryafterischemicstroke AT arnaboldifrancesca multiplexingbiosensorforthedetectionofextracellularvesiclesasbiomarkersoftissuedamageandrecoveryafterischemicstroke AT liuzzipiergiuseppe multiplexingbiosensorforthedetectionofextracellularvesiclesasbiomarkersoftissuedamageandrecoveryafterischemicstroke AT manniniandrea multiplexingbiosensorforthedetectionofextracellularvesiclesasbiomarkersoftissuedamageandrecoveryafterischemicstroke AT bedonimarzia multiplexingbiosensorforthedetectionofextracellularvesiclesasbiomarkersoftissuedamageandrecoveryafterischemicstroke AT gualerzialice multiplexingbiosensorforthedetectionofextracellularvesiclesasbiomarkersoftissuedamageandrecoveryafterischemicstroke |