Cargando…
Dissecting Rubella Placental Infection in an In Vitro Trophoblast Model
Vertical transmission of rubella virus (RuV) occurs at a high rate during the first trimester of pregnancy. The modes of vertical transmission including the response of trophoblasts to RuV are not well understood. Here, RuV-trophoblast interaction was studied in the BeWo trophoblast cell line. Analy...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10178045/ https://www.ncbi.nlm.nih.gov/pubmed/37175600 http://dx.doi.org/10.3390/ijms24097894 |
_version_ | 1785040766684364800 |
---|---|
author | Schulz, Juliane Schilling, Erik Fabian, Claire Zenclussen, Ana Claudia Stojanovska, Violeta Claus, Claudia |
author_facet | Schulz, Juliane Schilling, Erik Fabian, Claire Zenclussen, Ana Claudia Stojanovska, Violeta Claus, Claudia |
author_sort | Schulz, Juliane |
collection | PubMed |
description | Vertical transmission of rubella virus (RuV) occurs at a high rate during the first trimester of pregnancy. The modes of vertical transmission including the response of trophoblasts to RuV are not well understood. Here, RuV-trophoblast interaction was studied in the BeWo trophoblast cell line. Analysis included early and late time-point kinetics of virus infection rate and the antiviral innate immune response at mRNA and protein level. BeWo characteristics were addressed through metabolic activity by extracellular flux analysis and syncytiotrophoblast formation through incubation with forskolin. We found that RuV infection of BeWo led to profuse type III interferon (IFN) production. Transfecting trophoblast cells with dsRNA analog induced an increase in the production of type I IFN-β and type III IFNs; however, this did not occur in RuV-infected BeWo trophoblasts. IFN-β and to a lesser extent type III IFN-λ1 were inhibitory to RuV. While no significant metabolic alteration was detected, RuV infection reduced the cell number in the monolayer culture in comparison to the mock control and resulted in detached and floating cells. Syncytia formation restricted RuV infection. The use of BeWo as a relevant cell culture model for infection of trophoblasts highlights cytopathogenicity in the absence of a type I IFN response as a pathogenic alteration by RuV. |
format | Online Article Text |
id | pubmed-10178045 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101780452023-05-13 Dissecting Rubella Placental Infection in an In Vitro Trophoblast Model Schulz, Juliane Schilling, Erik Fabian, Claire Zenclussen, Ana Claudia Stojanovska, Violeta Claus, Claudia Int J Mol Sci Article Vertical transmission of rubella virus (RuV) occurs at a high rate during the first trimester of pregnancy. The modes of vertical transmission including the response of trophoblasts to RuV are not well understood. Here, RuV-trophoblast interaction was studied in the BeWo trophoblast cell line. Analysis included early and late time-point kinetics of virus infection rate and the antiviral innate immune response at mRNA and protein level. BeWo characteristics were addressed through metabolic activity by extracellular flux analysis and syncytiotrophoblast formation through incubation with forskolin. We found that RuV infection of BeWo led to profuse type III interferon (IFN) production. Transfecting trophoblast cells with dsRNA analog induced an increase in the production of type I IFN-β and type III IFNs; however, this did not occur in RuV-infected BeWo trophoblasts. IFN-β and to a lesser extent type III IFN-λ1 were inhibitory to RuV. While no significant metabolic alteration was detected, RuV infection reduced the cell number in the monolayer culture in comparison to the mock control and resulted in detached and floating cells. Syncytia formation restricted RuV infection. The use of BeWo as a relevant cell culture model for infection of trophoblasts highlights cytopathogenicity in the absence of a type I IFN response as a pathogenic alteration by RuV. MDPI 2023-04-26 /pmc/articles/PMC10178045/ /pubmed/37175600 http://dx.doi.org/10.3390/ijms24097894 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Schulz, Juliane Schilling, Erik Fabian, Claire Zenclussen, Ana Claudia Stojanovska, Violeta Claus, Claudia Dissecting Rubella Placental Infection in an In Vitro Trophoblast Model |
title | Dissecting Rubella Placental Infection in an In Vitro Trophoblast Model |
title_full | Dissecting Rubella Placental Infection in an In Vitro Trophoblast Model |
title_fullStr | Dissecting Rubella Placental Infection in an In Vitro Trophoblast Model |
title_full_unstemmed | Dissecting Rubella Placental Infection in an In Vitro Trophoblast Model |
title_short | Dissecting Rubella Placental Infection in an In Vitro Trophoblast Model |
title_sort | dissecting rubella placental infection in an in vitro trophoblast model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10178045/ https://www.ncbi.nlm.nih.gov/pubmed/37175600 http://dx.doi.org/10.3390/ijms24097894 |
work_keys_str_mv | AT schulzjuliane dissectingrubellaplacentalinfectioninaninvitrotrophoblastmodel AT schillingerik dissectingrubellaplacentalinfectioninaninvitrotrophoblastmodel AT fabianclaire dissectingrubellaplacentalinfectioninaninvitrotrophoblastmodel AT zenclussenanaclaudia dissectingrubellaplacentalinfectioninaninvitrotrophoblastmodel AT stojanovskavioleta dissectingrubellaplacentalinfectioninaninvitrotrophoblastmodel AT clausclaudia dissectingrubellaplacentalinfectioninaninvitrotrophoblastmodel |