NanoLuc Luciferase as a Fluorogen-Activating Protein for GFP Chromophore Based Fluorogens

In this work, we showed that the well-known NanoLuc luciferase can act as a fluorogen activating protein for various arylidene-imidazolones structurally similar to the Kaede protein chromophore. We showed that such compounds can be used as fluorescent sensors for this protein and can also be used in...

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Detalles Bibliográficos
Autores principales: Bogdanova, Yulia A., Zaitseva, Elvira R., Smirnov, Alexander Yu., Baleeva, Nadezhda S., Gavrikov, Alexey S., Myasnyanko, Ivan N., Goncharuk, Sergey A., Kot, Erik F., Mineev, Konstantin S., Mishin, Alexander S., Baranov, Mikhail S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10178166/
https://www.ncbi.nlm.nih.gov/pubmed/37175667
http://dx.doi.org/10.3390/ijms24097958
Descripción
Sumario:In this work, we showed that the well-known NanoLuc luciferase can act as a fluorogen activating protein for various arylidene-imidazolones structurally similar to the Kaede protein chromophore. We showed that such compounds can be used as fluorescent sensors for this protein and can also be used in pairs with it in fluorescent microscopy as a genetically encoded tag.

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