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NanoLuc Luciferase as a Fluorogen-Activating Protein for GFP Chromophore Based Fluorogens
In this work, we showed that the well-known NanoLuc luciferase can act as a fluorogen activating protein for various arylidene-imidazolones structurally similar to the Kaede protein chromophore. We showed that such compounds can be used as fluorescent sensors for this protein and can also be used in...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
MDPI
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10178166/ https://www.ncbi.nlm.nih.gov/pubmed/37175667 http://dx.doi.org/10.3390/ijms24097958 |
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| author | Bogdanova, Yulia A. Zaitseva, Elvira R. Smirnov, Alexander Yu. Baleeva, Nadezhda S. Gavrikov, Alexey S. Myasnyanko, Ivan N. Goncharuk, Sergey A. Kot, Erik F. Mineev, Konstantin S. Mishin, Alexander S. Baranov, Mikhail S. |
| author_facet | Bogdanova, Yulia A. Zaitseva, Elvira R. Smirnov, Alexander Yu. Baleeva, Nadezhda S. Gavrikov, Alexey S. Myasnyanko, Ivan N. Goncharuk, Sergey A. Kot, Erik F. Mineev, Konstantin S. Mishin, Alexander S. Baranov, Mikhail S. |
| author_sort | Bogdanova, Yulia A. |
| collection | PubMed |
| description | In this work, we showed that the well-known NanoLuc luciferase can act as a fluorogen activating protein for various arylidene-imidazolones structurally similar to the Kaede protein chromophore. We showed that such compounds can be used as fluorescent sensors for this protein and can also be used in pairs with it in fluorescent microscopy as a genetically encoded tag. |
| format | Online Article Text |
| id | pubmed-10178166 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2023 |
| publisher | MDPI |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-101781662023-05-13 NanoLuc Luciferase as a Fluorogen-Activating Protein for GFP Chromophore Based Fluorogens Bogdanova, Yulia A. Zaitseva, Elvira R. Smirnov, Alexander Yu. Baleeva, Nadezhda S. Gavrikov, Alexey S. Myasnyanko, Ivan N. Goncharuk, Sergey A. Kot, Erik F. Mineev, Konstantin S. Mishin, Alexander S. Baranov, Mikhail S. Int J Mol Sci Article In this work, we showed that the well-known NanoLuc luciferase can act as a fluorogen activating protein for various arylidene-imidazolones structurally similar to the Kaede protein chromophore. We showed that such compounds can be used as fluorescent sensors for this protein and can also be used in pairs with it in fluorescent microscopy as a genetically encoded tag. MDPI 2023-04-27 /pmc/articles/PMC10178166/ /pubmed/37175667 http://dx.doi.org/10.3390/ijms24097958 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
| spellingShingle | Article Bogdanova, Yulia A. Zaitseva, Elvira R. Smirnov, Alexander Yu. Baleeva, Nadezhda S. Gavrikov, Alexey S. Myasnyanko, Ivan N. Goncharuk, Sergey A. Kot, Erik F. Mineev, Konstantin S. Mishin, Alexander S. Baranov, Mikhail S. NanoLuc Luciferase as a Fluorogen-Activating Protein for GFP Chromophore Based Fluorogens |
| title | NanoLuc Luciferase as a Fluorogen-Activating Protein for GFP Chromophore Based Fluorogens |
| title_full | NanoLuc Luciferase as a Fluorogen-Activating Protein for GFP Chromophore Based Fluorogens |
| title_fullStr | NanoLuc Luciferase as a Fluorogen-Activating Protein for GFP Chromophore Based Fluorogens |
| title_full_unstemmed | NanoLuc Luciferase as a Fluorogen-Activating Protein for GFP Chromophore Based Fluorogens |
| title_short | NanoLuc Luciferase as a Fluorogen-Activating Protein for GFP Chromophore Based Fluorogens |
| title_sort | nanoluc luciferase as a fluorogen-activating protein for gfp chromophore based fluorogens |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10178166/ https://www.ncbi.nlm.nih.gov/pubmed/37175667 http://dx.doi.org/10.3390/ijms24097958 |
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