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Promotive Effect of FBXO32 on the Odontoblastic Differentiation of Human Dental Pulp Stem Cells

Odontoblastic differentiation of human dental pulp stem cells (hDPSCs) is crucial for the intricate formation and repair processes in dental pulp. Until now, the literature is not able to demonstrate the role of ubiquitination in the odontoblastic differentiation of hDPSCs. This study investigated t...

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Autores principales: Xu, Ke, Liu, Qin, Huang, Wushuang, Chu, Yanhao, Fan, Wenguo, Liu, Jiawei, He, Yifan, Huang, Fang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10178205/
https://www.ncbi.nlm.nih.gov/pubmed/37175415
http://dx.doi.org/10.3390/ijms24097708
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author Xu, Ke
Liu, Qin
Huang, Wushuang
Chu, Yanhao
Fan, Wenguo
Liu, Jiawei
He, Yifan
Huang, Fang
author_facet Xu, Ke
Liu, Qin
Huang, Wushuang
Chu, Yanhao
Fan, Wenguo
Liu, Jiawei
He, Yifan
Huang, Fang
author_sort Xu, Ke
collection PubMed
description Odontoblastic differentiation of human dental pulp stem cells (hDPSCs) is crucial for the intricate formation and repair processes in dental pulp. Until now, the literature is not able to demonstrate the role of ubiquitination in the odontoblastic differentiation of hDPSCs. This study investigated the role of F-box-only protein 32 (FBXO32), an E3 ligase, in the odontoblastic differentiation of hDPSCs. The mRNA expression profile was obtained from ribonucleic acid sequencing (RNA-Seq) data and analyzed. Immunofluorescence and immunohistochemical staining identify the FBXO32 expression in human dental pulp and hDPSCs. Small-hairpin RNA lentivirus was used for FBXO32 knockdown and overexpression. Odontoblastic differentiation of hDPSCs was determined via alkaline phosphatase activity, Alizarin Red S staining, and mRNA and protein expression levels were detected using real-time quantitative polymerase chain reaction and Western blotting. Furthermore, subcutaneous transplantation in nude mice was performed to evaluate the role of FBXO32 in mineralization in vivo using histological analysis. FBXO32 expression was upregulated in the odontoblast differentiated hDPSCs as evidenced by RNA-Seq data analysis. FBXO32 was detected in hDPSCs and the odontoblast layer of the dental pulp. Increased FBXO32 expression in hDPSCs during odontoblastic differentiation was confirmed. Through lentivirus infection method, FBXO32 downregulation in hDPSCs attenuated odontoblastic differentiation in vitro and in vivo, whereas FBXO32 upregulation promoted the hDPSCs odontoblastic differentiation, without affecting proliferation and migration. This study demonstrated, for the first time, the promotive role of FBXO32 in regulating the odontoblastic differentiation of hDPSCs, thereby providing novel insights into the regulatory mechanisms during odontoblastic differentiation in hDPSCs.
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spelling pubmed-101782052023-05-13 Promotive Effect of FBXO32 on the Odontoblastic Differentiation of Human Dental Pulp Stem Cells Xu, Ke Liu, Qin Huang, Wushuang Chu, Yanhao Fan, Wenguo Liu, Jiawei He, Yifan Huang, Fang Int J Mol Sci Article Odontoblastic differentiation of human dental pulp stem cells (hDPSCs) is crucial for the intricate formation and repair processes in dental pulp. Until now, the literature is not able to demonstrate the role of ubiquitination in the odontoblastic differentiation of hDPSCs. This study investigated the role of F-box-only protein 32 (FBXO32), an E3 ligase, in the odontoblastic differentiation of hDPSCs. The mRNA expression profile was obtained from ribonucleic acid sequencing (RNA-Seq) data and analyzed. Immunofluorescence and immunohistochemical staining identify the FBXO32 expression in human dental pulp and hDPSCs. Small-hairpin RNA lentivirus was used for FBXO32 knockdown and overexpression. Odontoblastic differentiation of hDPSCs was determined via alkaline phosphatase activity, Alizarin Red S staining, and mRNA and protein expression levels were detected using real-time quantitative polymerase chain reaction and Western blotting. Furthermore, subcutaneous transplantation in nude mice was performed to evaluate the role of FBXO32 in mineralization in vivo using histological analysis. FBXO32 expression was upregulated in the odontoblast differentiated hDPSCs as evidenced by RNA-Seq data analysis. FBXO32 was detected in hDPSCs and the odontoblast layer of the dental pulp. Increased FBXO32 expression in hDPSCs during odontoblastic differentiation was confirmed. Through lentivirus infection method, FBXO32 downregulation in hDPSCs attenuated odontoblastic differentiation in vitro and in vivo, whereas FBXO32 upregulation promoted the hDPSCs odontoblastic differentiation, without affecting proliferation and migration. This study demonstrated, for the first time, the promotive role of FBXO32 in regulating the odontoblastic differentiation of hDPSCs, thereby providing novel insights into the regulatory mechanisms during odontoblastic differentiation in hDPSCs. MDPI 2023-04-22 /pmc/articles/PMC10178205/ /pubmed/37175415 http://dx.doi.org/10.3390/ijms24097708 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xu, Ke
Liu, Qin
Huang, Wushuang
Chu, Yanhao
Fan, Wenguo
Liu, Jiawei
He, Yifan
Huang, Fang
Promotive Effect of FBXO32 on the Odontoblastic Differentiation of Human Dental Pulp Stem Cells
title Promotive Effect of FBXO32 on the Odontoblastic Differentiation of Human Dental Pulp Stem Cells
title_full Promotive Effect of FBXO32 on the Odontoblastic Differentiation of Human Dental Pulp Stem Cells
title_fullStr Promotive Effect of FBXO32 on the Odontoblastic Differentiation of Human Dental Pulp Stem Cells
title_full_unstemmed Promotive Effect of FBXO32 on the Odontoblastic Differentiation of Human Dental Pulp Stem Cells
title_short Promotive Effect of FBXO32 on the Odontoblastic Differentiation of Human Dental Pulp Stem Cells
title_sort promotive effect of fbxo32 on the odontoblastic differentiation of human dental pulp stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10178205/
https://www.ncbi.nlm.nih.gov/pubmed/37175415
http://dx.doi.org/10.3390/ijms24097708
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