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Polymorphism of Saccharomyces cerevisiae Strains in DNA Metabolism Genes

Baker’s yeast, S. cerevisiae, is an excellent model organism exploited for molecular genetic studies of the mechanisms of genome stability in eukaryotes. Genetic peculiarities of commonly used yeast strains impact the processes of DNA replication, repair, and recombination (RRR). We compared the gen...

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Detalles Bibliográficos
Autores principales: Zhuk, Anna S., Lada, Artem G., Pavlov, Youri I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10178279/
https://www.ncbi.nlm.nih.gov/pubmed/37175502
http://dx.doi.org/10.3390/ijms24097795
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author Zhuk, Anna S.
Lada, Artem G.
Pavlov, Youri I.
author_facet Zhuk, Anna S.
Lada, Artem G.
Pavlov, Youri I.
author_sort Zhuk, Anna S.
collection PubMed
description Baker’s yeast, S. cerevisiae, is an excellent model organism exploited for molecular genetic studies of the mechanisms of genome stability in eukaryotes. Genetic peculiarities of commonly used yeast strains impact the processes of DNA replication, repair, and recombination (RRR). We compared the genomic DNA sequence variation of the five strains that are intensively used for RRR studies. We used yeast next-generation sequencing data to detect the extent and significance of variation in 183 RRR genes. We present a detailed analysis of the differences that were found even in closely related strains. Polymorphisms of common yeast strains should be considered when interpreting the outcomes of genome stability studies, especially in cases of discrepancies between laboratories describing the same phenomena.
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spelling pubmed-101782792023-05-13 Polymorphism of Saccharomyces cerevisiae Strains in DNA Metabolism Genes Zhuk, Anna S. Lada, Artem G. Pavlov, Youri I. Int J Mol Sci Article Baker’s yeast, S. cerevisiae, is an excellent model organism exploited for molecular genetic studies of the mechanisms of genome stability in eukaryotes. Genetic peculiarities of commonly used yeast strains impact the processes of DNA replication, repair, and recombination (RRR). We compared the genomic DNA sequence variation of the five strains that are intensively used for RRR studies. We used yeast next-generation sequencing data to detect the extent and significance of variation in 183 RRR genes. We present a detailed analysis of the differences that were found even in closely related strains. Polymorphisms of common yeast strains should be considered when interpreting the outcomes of genome stability studies, especially in cases of discrepancies between laboratories describing the same phenomena. MDPI 2023-04-25 /pmc/articles/PMC10178279/ /pubmed/37175502 http://dx.doi.org/10.3390/ijms24097795 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhuk, Anna S.
Lada, Artem G.
Pavlov, Youri I.
Polymorphism of Saccharomyces cerevisiae Strains in DNA Metabolism Genes
title Polymorphism of Saccharomyces cerevisiae Strains in DNA Metabolism Genes
title_full Polymorphism of Saccharomyces cerevisiae Strains in DNA Metabolism Genes
title_fullStr Polymorphism of Saccharomyces cerevisiae Strains in DNA Metabolism Genes
title_full_unstemmed Polymorphism of Saccharomyces cerevisiae Strains in DNA Metabolism Genes
title_short Polymorphism of Saccharomyces cerevisiae Strains in DNA Metabolism Genes
title_sort polymorphism of saccharomyces cerevisiae strains in dna metabolism genes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10178279/
https://www.ncbi.nlm.nih.gov/pubmed/37175502
http://dx.doi.org/10.3390/ijms24097795
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AT pavlovyourii polymorphismofsaccharomycescerevisiaestrainsindnametabolismgenes