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Polymorphism of Saccharomyces cerevisiae Strains in DNA Metabolism Genes
Baker’s yeast, S. cerevisiae, is an excellent model organism exploited for molecular genetic studies of the mechanisms of genome stability in eukaryotes. Genetic peculiarities of commonly used yeast strains impact the processes of DNA replication, repair, and recombination (RRR). We compared the gen...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10178279/ https://www.ncbi.nlm.nih.gov/pubmed/37175502 http://dx.doi.org/10.3390/ijms24097795 |
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author | Zhuk, Anna S. Lada, Artem G. Pavlov, Youri I. |
author_facet | Zhuk, Anna S. Lada, Artem G. Pavlov, Youri I. |
author_sort | Zhuk, Anna S. |
collection | PubMed |
description | Baker’s yeast, S. cerevisiae, is an excellent model organism exploited for molecular genetic studies of the mechanisms of genome stability in eukaryotes. Genetic peculiarities of commonly used yeast strains impact the processes of DNA replication, repair, and recombination (RRR). We compared the genomic DNA sequence variation of the five strains that are intensively used for RRR studies. We used yeast next-generation sequencing data to detect the extent and significance of variation in 183 RRR genes. We present a detailed analysis of the differences that were found even in closely related strains. Polymorphisms of common yeast strains should be considered when interpreting the outcomes of genome stability studies, especially in cases of discrepancies between laboratories describing the same phenomena. |
format | Online Article Text |
id | pubmed-10178279 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101782792023-05-13 Polymorphism of Saccharomyces cerevisiae Strains in DNA Metabolism Genes Zhuk, Anna S. Lada, Artem G. Pavlov, Youri I. Int J Mol Sci Article Baker’s yeast, S. cerevisiae, is an excellent model organism exploited for molecular genetic studies of the mechanisms of genome stability in eukaryotes. Genetic peculiarities of commonly used yeast strains impact the processes of DNA replication, repair, and recombination (RRR). We compared the genomic DNA sequence variation of the five strains that are intensively used for RRR studies. We used yeast next-generation sequencing data to detect the extent and significance of variation in 183 RRR genes. We present a detailed analysis of the differences that were found even in closely related strains. Polymorphisms of common yeast strains should be considered when interpreting the outcomes of genome stability studies, especially in cases of discrepancies between laboratories describing the same phenomena. MDPI 2023-04-25 /pmc/articles/PMC10178279/ /pubmed/37175502 http://dx.doi.org/10.3390/ijms24097795 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhuk, Anna S. Lada, Artem G. Pavlov, Youri I. Polymorphism of Saccharomyces cerevisiae Strains in DNA Metabolism Genes |
title | Polymorphism of Saccharomyces cerevisiae Strains in DNA Metabolism Genes |
title_full | Polymorphism of Saccharomyces cerevisiae Strains in DNA Metabolism Genes |
title_fullStr | Polymorphism of Saccharomyces cerevisiae Strains in DNA Metabolism Genes |
title_full_unstemmed | Polymorphism of Saccharomyces cerevisiae Strains in DNA Metabolism Genes |
title_short | Polymorphism of Saccharomyces cerevisiae Strains in DNA Metabolism Genes |
title_sort | polymorphism of saccharomyces cerevisiae strains in dna metabolism genes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10178279/ https://www.ncbi.nlm.nih.gov/pubmed/37175502 http://dx.doi.org/10.3390/ijms24097795 |
work_keys_str_mv | AT zhukannas polymorphismofsaccharomycescerevisiaestrainsindnametabolismgenes AT ladaartemg polymorphismofsaccharomycescerevisiaestrainsindnametabolismgenes AT pavlovyourii polymorphismofsaccharomycescerevisiaestrainsindnametabolismgenes |