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Shiga Toxin, Stx2e, Influences the Activity of Porcine Lymphocytes In Vitro
Oedema disease (OD) in piglets is one of the most important pathologies, as it causes significant losses due to the high mortality because of the Shiga toxin family, which produces Escherichia coli (STEC) strains. The main toxin responsible for the characteristic pathologies in pigs is Shiga toxin 2...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10178452/ https://www.ncbi.nlm.nih.gov/pubmed/37175714 http://dx.doi.org/10.3390/ijms24098009 |
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author | Sperling, Daniel Stepanova, Hana Smits, Han Diesing, Anne-Kathrin Faldyna, Martin |
author_facet | Sperling, Daniel Stepanova, Hana Smits, Han Diesing, Anne-Kathrin Faldyna, Martin |
author_sort | Sperling, Daniel |
collection | PubMed |
description | Oedema disease (OD) in piglets is one of the most important pathologies, as it causes significant losses due to the high mortality because of the Shiga toxin family, which produces Escherichia coli (STEC) strains. The main toxin responsible for the characteristic pathologies in pigs is Shiga toxin 2 subtype e (Stx2e). Moreover, there is growing evidence that Stx’s family of toxins also targets immune cells. Therefore, this study evaluated the effect of different concentrations of Stx2e on porcine immune cells. Porcine peripheral blood mononuclear cells were pre-incubated with Stx2e, at three different concentrations (final concentrations of 10, 500, and 5000 CD50/mL) and with a negative control group. Cells were then stimulated with polyclonal mitogens: concanavalin A, phytohemagglutinin, pokeweed mitogen, or lipopolysaccharides. Cell proliferation was assessed by BrdU (or EdU) incorporation into newly created DNA. The activation of the lymphocyte subsets was assessed by the detection of CD25, using flow cytometry. The toxin significantly decreased mitogen-driven proliferation activity, and the effect was partially dose-dependent, with a significant impact on both T and B populations. The percentage of CD25+ cells was slightly lower in the presence of Stx2e in all the defined T cell subpopulations (CD4+, CD8+, and γδTCR+)—in a dose-dependent manner. B cells seemed to be the most affected populations. The negative effects of different concentrations of Stx2e on the immune cells in this study may explain the negative impact of the subclinical course of OD. |
format | Online Article Text |
id | pubmed-10178452 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101784522023-05-13 Shiga Toxin, Stx2e, Influences the Activity of Porcine Lymphocytes In Vitro Sperling, Daniel Stepanova, Hana Smits, Han Diesing, Anne-Kathrin Faldyna, Martin Int J Mol Sci Article Oedema disease (OD) in piglets is one of the most important pathologies, as it causes significant losses due to the high mortality because of the Shiga toxin family, which produces Escherichia coli (STEC) strains. The main toxin responsible for the characteristic pathologies in pigs is Shiga toxin 2 subtype e (Stx2e). Moreover, there is growing evidence that Stx’s family of toxins also targets immune cells. Therefore, this study evaluated the effect of different concentrations of Stx2e on porcine immune cells. Porcine peripheral blood mononuclear cells were pre-incubated with Stx2e, at three different concentrations (final concentrations of 10, 500, and 5000 CD50/mL) and with a negative control group. Cells were then stimulated with polyclonal mitogens: concanavalin A, phytohemagglutinin, pokeweed mitogen, or lipopolysaccharides. Cell proliferation was assessed by BrdU (or EdU) incorporation into newly created DNA. The activation of the lymphocyte subsets was assessed by the detection of CD25, using flow cytometry. The toxin significantly decreased mitogen-driven proliferation activity, and the effect was partially dose-dependent, with a significant impact on both T and B populations. The percentage of CD25+ cells was slightly lower in the presence of Stx2e in all the defined T cell subpopulations (CD4+, CD8+, and γδTCR+)—in a dose-dependent manner. B cells seemed to be the most affected populations. The negative effects of different concentrations of Stx2e on the immune cells in this study may explain the negative impact of the subclinical course of OD. MDPI 2023-04-28 /pmc/articles/PMC10178452/ /pubmed/37175714 http://dx.doi.org/10.3390/ijms24098009 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sperling, Daniel Stepanova, Hana Smits, Han Diesing, Anne-Kathrin Faldyna, Martin Shiga Toxin, Stx2e, Influences the Activity of Porcine Lymphocytes In Vitro |
title | Shiga Toxin, Stx2e, Influences the Activity of Porcine Lymphocytes In Vitro |
title_full | Shiga Toxin, Stx2e, Influences the Activity of Porcine Lymphocytes In Vitro |
title_fullStr | Shiga Toxin, Stx2e, Influences the Activity of Porcine Lymphocytes In Vitro |
title_full_unstemmed | Shiga Toxin, Stx2e, Influences the Activity of Porcine Lymphocytes In Vitro |
title_short | Shiga Toxin, Stx2e, Influences the Activity of Porcine Lymphocytes In Vitro |
title_sort | shiga toxin, stx2e, influences the activity of porcine lymphocytes in vitro |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10178452/ https://www.ncbi.nlm.nih.gov/pubmed/37175714 http://dx.doi.org/10.3390/ijms24098009 |
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