Cargando…
Cyclic Stretch-Induced Mechanical Stress Applied at 1 Hz Frequency Can Alter the Metastatic Potential Properties of SAOS-2 Osteosarcoma Cells
Recently, there has been an increasing focus on cellular morphology and mechanical behavior in order to gain a better understanding of the modulation of cell malignancy. This study used uniaxial-stretching technology to select a mechanical regimen able to elevate SAOS-2 cell migration, which is cruc...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10178551/ https://www.ncbi.nlm.nih.gov/pubmed/37175397 http://dx.doi.org/10.3390/ijms24097686 |
_version_ | 1785040891942010880 |
---|---|
author | Alloisio, Giulia Rodriguez, David Becerril Luce, Marco Ciaccio, Chiara Marini, Stefano Cricenti, Antonio Gioia, Magda |
author_facet | Alloisio, Giulia Rodriguez, David Becerril Luce, Marco Ciaccio, Chiara Marini, Stefano Cricenti, Antonio Gioia, Magda |
author_sort | Alloisio, Giulia |
collection | PubMed |
description | Recently, there has been an increasing focus on cellular morphology and mechanical behavior in order to gain a better understanding of the modulation of cell malignancy. This study used uniaxial-stretching technology to select a mechanical regimen able to elevate SAOS-2 cell migration, which is crucial in osteosarcoma cell pathology. Using confocal and atomic force microscopy, we demonstrated that a 24 h 0.5% cyclic elongation applied at 1 Hz induces morphological changes in cells. Following mechanical stimulation, the cell area enlarged, developing a more elongated shape, which disrupted the initial nuclear-to-cytoplasm ratio. The peripheral cell surface also increased its roughness. Cell-based biochemical assays and real-time PCR quantification showed that these morphologically induced changes are unrelated to the osteoblastic differentiative grade. Interestingly, two essential cell-motility properties in the modulation of the metastatic process changed following the 24 h 1 Hz mechanical stimulation. These were cell adhesion and cell migration, which, in fact, were dampened and enhanced, respectively. Notably, our results showed that the stretch-induced up-regulation of cell motility occurs through a mechanism that does not depend on matrix metalloproteinase (MMP) activity, while the inhibition of ion–stretch channels could counteract it. Overall, our results suggest that further research on mechanobiology could represent an alternative approach for the identification of novel molecular targets of osteosarcoma cell malignancy. |
format | Online Article Text |
id | pubmed-10178551 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101785512023-05-13 Cyclic Stretch-Induced Mechanical Stress Applied at 1 Hz Frequency Can Alter the Metastatic Potential Properties of SAOS-2 Osteosarcoma Cells Alloisio, Giulia Rodriguez, David Becerril Luce, Marco Ciaccio, Chiara Marini, Stefano Cricenti, Antonio Gioia, Magda Int J Mol Sci Article Recently, there has been an increasing focus on cellular morphology and mechanical behavior in order to gain a better understanding of the modulation of cell malignancy. This study used uniaxial-stretching technology to select a mechanical regimen able to elevate SAOS-2 cell migration, which is crucial in osteosarcoma cell pathology. Using confocal and atomic force microscopy, we demonstrated that a 24 h 0.5% cyclic elongation applied at 1 Hz induces morphological changes in cells. Following mechanical stimulation, the cell area enlarged, developing a more elongated shape, which disrupted the initial nuclear-to-cytoplasm ratio. The peripheral cell surface also increased its roughness. Cell-based biochemical assays and real-time PCR quantification showed that these morphologically induced changes are unrelated to the osteoblastic differentiative grade. Interestingly, two essential cell-motility properties in the modulation of the metastatic process changed following the 24 h 1 Hz mechanical stimulation. These were cell adhesion and cell migration, which, in fact, were dampened and enhanced, respectively. Notably, our results showed that the stretch-induced up-regulation of cell motility occurs through a mechanism that does not depend on matrix metalloproteinase (MMP) activity, while the inhibition of ion–stretch channels could counteract it. Overall, our results suggest that further research on mechanobiology could represent an alternative approach for the identification of novel molecular targets of osteosarcoma cell malignancy. MDPI 2023-04-22 /pmc/articles/PMC10178551/ /pubmed/37175397 http://dx.doi.org/10.3390/ijms24097686 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Alloisio, Giulia Rodriguez, David Becerril Luce, Marco Ciaccio, Chiara Marini, Stefano Cricenti, Antonio Gioia, Magda Cyclic Stretch-Induced Mechanical Stress Applied at 1 Hz Frequency Can Alter the Metastatic Potential Properties of SAOS-2 Osteosarcoma Cells |
title | Cyclic Stretch-Induced Mechanical Stress Applied at 1 Hz Frequency Can Alter the Metastatic Potential Properties of SAOS-2 Osteosarcoma Cells |
title_full | Cyclic Stretch-Induced Mechanical Stress Applied at 1 Hz Frequency Can Alter the Metastatic Potential Properties of SAOS-2 Osteosarcoma Cells |
title_fullStr | Cyclic Stretch-Induced Mechanical Stress Applied at 1 Hz Frequency Can Alter the Metastatic Potential Properties of SAOS-2 Osteosarcoma Cells |
title_full_unstemmed | Cyclic Stretch-Induced Mechanical Stress Applied at 1 Hz Frequency Can Alter the Metastatic Potential Properties of SAOS-2 Osteosarcoma Cells |
title_short | Cyclic Stretch-Induced Mechanical Stress Applied at 1 Hz Frequency Can Alter the Metastatic Potential Properties of SAOS-2 Osteosarcoma Cells |
title_sort | cyclic stretch-induced mechanical stress applied at 1 hz frequency can alter the metastatic potential properties of saos-2 osteosarcoma cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10178551/ https://www.ncbi.nlm.nih.gov/pubmed/37175397 http://dx.doi.org/10.3390/ijms24097686 |
work_keys_str_mv | AT alloisiogiulia cyclicstretchinducedmechanicalstressappliedat1hzfrequencycanalterthemetastaticpotentialpropertiesofsaos2osteosarcomacells AT rodriguezdavidbecerril cyclicstretchinducedmechanicalstressappliedat1hzfrequencycanalterthemetastaticpotentialpropertiesofsaos2osteosarcomacells AT lucemarco cyclicstretchinducedmechanicalstressappliedat1hzfrequencycanalterthemetastaticpotentialpropertiesofsaos2osteosarcomacells AT ciacciochiara cyclicstretchinducedmechanicalstressappliedat1hzfrequencycanalterthemetastaticpotentialpropertiesofsaos2osteosarcomacells AT marinistefano cyclicstretchinducedmechanicalstressappliedat1hzfrequencycanalterthemetastaticpotentialpropertiesofsaos2osteosarcomacells AT cricentiantonio cyclicstretchinducedmechanicalstressappliedat1hzfrequencycanalterthemetastaticpotentialpropertiesofsaos2osteosarcomacells AT gioiamagda cyclicstretchinducedmechanicalstressappliedat1hzfrequencycanalterthemetastaticpotentialpropertiesofsaos2osteosarcomacells |