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OsMADS1 Regulates Grain Quality, Gene Expressions, and Regulatory Networks of Starch and Storage Protein Metabolisms in Rice
OsMADS1 plays a vital role in regulating floret development and grain shape, but whether it regulates rice grain quality still remains largely unknown. Therefore, we used comprehensive molecular genetics, plant biotechnology, and functional omics approaches, including phenotyping, mapping-by-sequenc...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10178960/ https://www.ncbi.nlm.nih.gov/pubmed/37175747 http://dx.doi.org/10.3390/ijms24098017 |
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author | Liu, Zhijian Li, Penghui Yu, Lan Hu, Yongzhi Du, Anping Fu, Xingyue Wu, Cuili Luo, Dagang Hu, Binhua Dong, Hui Jiang, Haibo Ma, Xinrong Huang, Weizao Yang, Xiaocheng Tu, Shengbin Li, Hui |
author_facet | Liu, Zhijian Li, Penghui Yu, Lan Hu, Yongzhi Du, Anping Fu, Xingyue Wu, Cuili Luo, Dagang Hu, Binhua Dong, Hui Jiang, Haibo Ma, Xinrong Huang, Weizao Yang, Xiaocheng Tu, Shengbin Li, Hui |
author_sort | Liu, Zhijian |
collection | PubMed |
description | OsMADS1 plays a vital role in regulating floret development and grain shape, but whether it regulates rice grain quality still remains largely unknown. Therefore, we used comprehensive molecular genetics, plant biotechnology, and functional omics approaches, including phenotyping, mapping-by-sequencing, target gene seed-specific RNAi, transgenic experiments, and transcriptomic profiling to answer this biological and molecular question. Here, we report the characterization of the ‘Oat-like rice’ mutant, with poor grain quality, including chalky endosperms, abnormal morphology and loose arrangement of starch granules, and lower starch content but higher protein content in grains. The poor grain quality of Oat-like rice was found to be caused by the mutated OsMADS1(Olr) allele through mapping-by-sequencing analysis and transgenic experiments. OsMADS1 protein is highly expressed in florets and developing seeds. Both OsMADS1-eGFP and OsMADS1(Olr)-eGFP fusion proteins are localized in the nucleus. Moreover, seed-specific RNAi of OsMADS1 also caused decreased grain quality in transgenic lines, such as the Oat-like rice. Further transcriptomic profiling between Oat-like rice and Nipponbare grains revealed that OsMADS1 regulates gene expressions and regulatory networks of starch and storage protein metabolisms in rice grains, hereafter regulating rice quality. In conclusion, our results not only reveal the crucial role and preliminary mechanism of OsMADS1 in regulating rice grain quality but also highlight the application potentials of OsMADS1 and the target gene seed-specific RNAi system in improving rice grain quality by molecular breeding. |
format | Online Article Text |
id | pubmed-10178960 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101789602023-05-13 OsMADS1 Regulates Grain Quality, Gene Expressions, and Regulatory Networks of Starch and Storage Protein Metabolisms in Rice Liu, Zhijian Li, Penghui Yu, Lan Hu, Yongzhi Du, Anping Fu, Xingyue Wu, Cuili Luo, Dagang Hu, Binhua Dong, Hui Jiang, Haibo Ma, Xinrong Huang, Weizao Yang, Xiaocheng Tu, Shengbin Li, Hui Int J Mol Sci Article OsMADS1 plays a vital role in regulating floret development and grain shape, but whether it regulates rice grain quality still remains largely unknown. Therefore, we used comprehensive molecular genetics, plant biotechnology, and functional omics approaches, including phenotyping, mapping-by-sequencing, target gene seed-specific RNAi, transgenic experiments, and transcriptomic profiling to answer this biological and molecular question. Here, we report the characterization of the ‘Oat-like rice’ mutant, with poor grain quality, including chalky endosperms, abnormal morphology and loose arrangement of starch granules, and lower starch content but higher protein content in grains. The poor grain quality of Oat-like rice was found to be caused by the mutated OsMADS1(Olr) allele through mapping-by-sequencing analysis and transgenic experiments. OsMADS1 protein is highly expressed in florets and developing seeds. Both OsMADS1-eGFP and OsMADS1(Olr)-eGFP fusion proteins are localized in the nucleus. Moreover, seed-specific RNAi of OsMADS1 also caused decreased grain quality in transgenic lines, such as the Oat-like rice. Further transcriptomic profiling between Oat-like rice and Nipponbare grains revealed that OsMADS1 regulates gene expressions and regulatory networks of starch and storage protein metabolisms in rice grains, hereafter regulating rice quality. In conclusion, our results not only reveal the crucial role and preliminary mechanism of OsMADS1 in regulating rice grain quality but also highlight the application potentials of OsMADS1 and the target gene seed-specific RNAi system in improving rice grain quality by molecular breeding. MDPI 2023-04-28 /pmc/articles/PMC10178960/ /pubmed/37175747 http://dx.doi.org/10.3390/ijms24098017 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Liu, Zhijian Li, Penghui Yu, Lan Hu, Yongzhi Du, Anping Fu, Xingyue Wu, Cuili Luo, Dagang Hu, Binhua Dong, Hui Jiang, Haibo Ma, Xinrong Huang, Weizao Yang, Xiaocheng Tu, Shengbin Li, Hui OsMADS1 Regulates Grain Quality, Gene Expressions, and Regulatory Networks of Starch and Storage Protein Metabolisms in Rice |
title | OsMADS1 Regulates Grain Quality, Gene Expressions, and Regulatory Networks of Starch and Storage Protein Metabolisms in Rice |
title_full | OsMADS1 Regulates Grain Quality, Gene Expressions, and Regulatory Networks of Starch and Storage Protein Metabolisms in Rice |
title_fullStr | OsMADS1 Regulates Grain Quality, Gene Expressions, and Regulatory Networks of Starch and Storage Protein Metabolisms in Rice |
title_full_unstemmed | OsMADS1 Regulates Grain Quality, Gene Expressions, and Regulatory Networks of Starch and Storage Protein Metabolisms in Rice |
title_short | OsMADS1 Regulates Grain Quality, Gene Expressions, and Regulatory Networks of Starch and Storage Protein Metabolisms in Rice |
title_sort | osmads1 regulates grain quality, gene expressions, and regulatory networks of starch and storage protein metabolisms in rice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10178960/ https://www.ncbi.nlm.nih.gov/pubmed/37175747 http://dx.doi.org/10.3390/ijms24098017 |
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