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Efficacy of a Multicomponent Nutraceutical Formulation for the Prevention and Treatment of Urinary Tract Stones

Urolithiasis is a complex and multifactorial disease characterized by the formation of calculi at the urinary tract level. Conventional therapeutic prophylaxis relies on the use of Ca-blockers, alkalis, diuretics, and anti-edema agents, but their prolonged utilization is often limited by several sid...

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Autores principales: Maisto, Maria, Schiano, Elisabetta, Luccheo, Gianni, Luccheo, Luigi, Alfieri, Ernesto, Piccolo, Vincenzo, Iannuzzo, Fortuna, Di Lorenzo, Ritamaria, Tenore, Gian Carlo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10178978/
https://www.ncbi.nlm.nih.gov/pubmed/37176022
http://dx.doi.org/10.3390/ijms24098316
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author Maisto, Maria
Schiano, Elisabetta
Luccheo, Gianni
Luccheo, Luigi
Alfieri, Ernesto
Piccolo, Vincenzo
Iannuzzo, Fortuna
Di Lorenzo, Ritamaria
Tenore, Gian Carlo
author_facet Maisto, Maria
Schiano, Elisabetta
Luccheo, Gianni
Luccheo, Luigi
Alfieri, Ernesto
Piccolo, Vincenzo
Iannuzzo, Fortuna
Di Lorenzo, Ritamaria
Tenore, Gian Carlo
author_sort Maisto, Maria
collection PubMed
description Urolithiasis is a complex and multifactorial disease characterized by the formation of calculi at the urinary tract level. Conventional therapeutic prophylaxis relies on the use of Ca-blockers, alkalis, diuretics, and anti-edema agents, but their prolonged utilization is often limited by several side effects. In this scenario, the aim of the present work was the design of an innovative multi-component nutraceutical formulation (NF) for the management of urinary stones consisting of a synergistic combination of natural aqueous extracts of Oreganum vulgare L. (1% of saponin), Urtica dioica (0.8% of β-sitosterol), Phyllanthus niruri (15% of tannins w/w), and Ceterach officinarum in association with bromelain, K, and Mg citrate. To assess the potential of NF also in the treatment of uric acid (UA) stones, the effects on the expression of the cellular UA transporters OAT1 and URAT1 were investigated in a renal tubular cell line. In addition, the myorelaxant effect of NF was investigated in a human pulmonary artery smooth muscle cell (HPASMC) model resulting in a decreased muscle contractility of −49.4% (p < 0.01) compared to the control. The treatment with NF also showed a valuable inhibition of in vitro calcium-oxalate crystal formation, both in prevention (−52.3% vs. control, p < 0.01) and treatment (−70.8% vs. control, p < 0.01) experiments. Finally, an ischemic reperfusion rat model was used to evaluate the NF anti-edema effects, resulting in a reduction in the edema-related vascular permeability (Normalized Gray Levels, NGL = 0.40 ± 0.09, p < 0.01, −67.1% vs. untreated rats). In conclusion, the present NF has shown to be a promising natural alternative for managing urinary tract stones.
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spelling pubmed-101789782023-05-13 Efficacy of a Multicomponent Nutraceutical Formulation for the Prevention and Treatment of Urinary Tract Stones Maisto, Maria Schiano, Elisabetta Luccheo, Gianni Luccheo, Luigi Alfieri, Ernesto Piccolo, Vincenzo Iannuzzo, Fortuna Di Lorenzo, Ritamaria Tenore, Gian Carlo Int J Mol Sci Article Urolithiasis is a complex and multifactorial disease characterized by the formation of calculi at the urinary tract level. Conventional therapeutic prophylaxis relies on the use of Ca-blockers, alkalis, diuretics, and anti-edema agents, but their prolonged utilization is often limited by several side effects. In this scenario, the aim of the present work was the design of an innovative multi-component nutraceutical formulation (NF) for the management of urinary stones consisting of a synergistic combination of natural aqueous extracts of Oreganum vulgare L. (1% of saponin), Urtica dioica (0.8% of β-sitosterol), Phyllanthus niruri (15% of tannins w/w), and Ceterach officinarum in association with bromelain, K, and Mg citrate. To assess the potential of NF also in the treatment of uric acid (UA) stones, the effects on the expression of the cellular UA transporters OAT1 and URAT1 were investigated in a renal tubular cell line. In addition, the myorelaxant effect of NF was investigated in a human pulmonary artery smooth muscle cell (HPASMC) model resulting in a decreased muscle contractility of −49.4% (p < 0.01) compared to the control. The treatment with NF also showed a valuable inhibition of in vitro calcium-oxalate crystal formation, both in prevention (−52.3% vs. control, p < 0.01) and treatment (−70.8% vs. control, p < 0.01) experiments. Finally, an ischemic reperfusion rat model was used to evaluate the NF anti-edema effects, resulting in a reduction in the edema-related vascular permeability (Normalized Gray Levels, NGL = 0.40 ± 0.09, p < 0.01, −67.1% vs. untreated rats). In conclusion, the present NF has shown to be a promising natural alternative for managing urinary tract stones. MDPI 2023-05-05 /pmc/articles/PMC10178978/ /pubmed/37176022 http://dx.doi.org/10.3390/ijms24098316 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Maisto, Maria
Schiano, Elisabetta
Luccheo, Gianni
Luccheo, Luigi
Alfieri, Ernesto
Piccolo, Vincenzo
Iannuzzo, Fortuna
Di Lorenzo, Ritamaria
Tenore, Gian Carlo
Efficacy of a Multicomponent Nutraceutical Formulation for the Prevention and Treatment of Urinary Tract Stones
title Efficacy of a Multicomponent Nutraceutical Formulation for the Prevention and Treatment of Urinary Tract Stones
title_full Efficacy of a Multicomponent Nutraceutical Formulation for the Prevention and Treatment of Urinary Tract Stones
title_fullStr Efficacy of a Multicomponent Nutraceutical Formulation for the Prevention and Treatment of Urinary Tract Stones
title_full_unstemmed Efficacy of a Multicomponent Nutraceutical Formulation for the Prevention and Treatment of Urinary Tract Stones
title_short Efficacy of a Multicomponent Nutraceutical Formulation for the Prevention and Treatment of Urinary Tract Stones
title_sort efficacy of a multicomponent nutraceutical formulation for the prevention and treatment of urinary tract stones
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10178978/
https://www.ncbi.nlm.nih.gov/pubmed/37176022
http://dx.doi.org/10.3390/ijms24098316
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