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A Split-Marker System for CRISPR-Cas9 Genome Editing in Methylotrophic Yeasts
Methylotrophic yeasts such as Ogataea polymorpha and Komagataella phaffii (sin. Hansenula polymorpha and Pichia pastoris, respectively) are commonly used in basic research and biotechnological applications, frequently those requiring genome modifications. However, the CRISPR-Cas9 genome editing appr...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10179152/ https://www.ncbi.nlm.nih.gov/pubmed/37175878 http://dx.doi.org/10.3390/ijms24098173 |