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A Split-Marker System for CRISPR-Cas9 Genome Editing in Methylotrophic Yeasts

Methylotrophic yeasts such as Ogataea polymorpha and Komagataella phaffii (sin. Hansenula polymorpha and Pichia pastoris, respectively) are commonly used in basic research and biotechnological applications, frequently those requiring genome modifications. However, the CRISPR-Cas9 genome editing appr...

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Detalles Bibliográficos
Autores principales:	Karginov, Azamat V., Tarutina, Marina G., Lapteva, Anastasia R., Pakhomova, Maria D., Galliamov, Artur A., Filkin, Sergey Y., Fedorov, Alexey N., Agaphonov, Michael O.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10179152/ https://www.ncbi.nlm.nih.gov/pubmed/37175878 http://dx.doi.org/10.3390/ijms24098173

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