Cargando…

Animal Models for the Investigation of P2X7 Receptors

The P2X7 receptor is a trimeric ligand-gated cation channel activated by extracellular adenosine 5′-triphosphate. The study of animals has greatly advanced the investigation of P2X7 and helped to establish the numerous physiological and pathophysiological roles of this receptor in human health and d...

Descripción completa

Detalles Bibliográficos
Autores principales: Sluyter, Ronald, Adriouch, Sahil, Fuller, Stephen J., Nicke, Annette, Sophocleous, Reece A., Watson, Debbie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10179175/
https://www.ncbi.nlm.nih.gov/pubmed/37175933
http://dx.doi.org/10.3390/ijms24098225
_version_ 1785041036151619584
author Sluyter, Ronald
Adriouch, Sahil
Fuller, Stephen J.
Nicke, Annette
Sophocleous, Reece A.
Watson, Debbie
author_facet Sluyter, Ronald
Adriouch, Sahil
Fuller, Stephen J.
Nicke, Annette
Sophocleous, Reece A.
Watson, Debbie
author_sort Sluyter, Ronald
collection PubMed
description The P2X7 receptor is a trimeric ligand-gated cation channel activated by extracellular adenosine 5′-triphosphate. The study of animals has greatly advanced the investigation of P2X7 and helped to establish the numerous physiological and pathophysiological roles of this receptor in human health and disease. Following a short overview of the P2X7 distribution, roles and functional properties, this article discusses how animal models have contributed to the generation of P2X7-specific antibodies and nanobodies (including biologics), recombinant receptors and radioligands to study P2X7 as well as to the pharmacokinetic testing of P2X7 antagonists. This article then outlines how mouse and rat models have been used to study P2X7. These sections include discussions on preclinical disease models, polymorphic P2X7 variants, P2X7 knockout mice (including bone marrow chimeras and conditional knockouts), P2X7 reporter mice, humanized P2X7 mice and P2X7 knockout rats. Finally, this article reviews the limited number of studies involving guinea pigs, rabbits, monkeys (rhesus macaques), dogs, cats, zebrafish, and other fish species (seabream, ayu sweetfish, rainbow trout and Japanese flounder) to study P2X7.
format Online
Article
Text
id pubmed-10179175
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-101791752023-05-13 Animal Models for the Investigation of P2X7 Receptors Sluyter, Ronald Adriouch, Sahil Fuller, Stephen J. Nicke, Annette Sophocleous, Reece A. Watson, Debbie Int J Mol Sci Review The P2X7 receptor is a trimeric ligand-gated cation channel activated by extracellular adenosine 5′-triphosphate. The study of animals has greatly advanced the investigation of P2X7 and helped to establish the numerous physiological and pathophysiological roles of this receptor in human health and disease. Following a short overview of the P2X7 distribution, roles and functional properties, this article discusses how animal models have contributed to the generation of P2X7-specific antibodies and nanobodies (including biologics), recombinant receptors and radioligands to study P2X7 as well as to the pharmacokinetic testing of P2X7 antagonists. This article then outlines how mouse and rat models have been used to study P2X7. These sections include discussions on preclinical disease models, polymorphic P2X7 variants, P2X7 knockout mice (including bone marrow chimeras and conditional knockouts), P2X7 reporter mice, humanized P2X7 mice and P2X7 knockout rats. Finally, this article reviews the limited number of studies involving guinea pigs, rabbits, monkeys (rhesus macaques), dogs, cats, zebrafish, and other fish species (seabream, ayu sweetfish, rainbow trout and Japanese flounder) to study P2X7. MDPI 2023-05-04 /pmc/articles/PMC10179175/ /pubmed/37175933 http://dx.doi.org/10.3390/ijms24098225 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Sluyter, Ronald
Adriouch, Sahil
Fuller, Stephen J.
Nicke, Annette
Sophocleous, Reece A.
Watson, Debbie
Animal Models for the Investigation of P2X7 Receptors
title Animal Models for the Investigation of P2X7 Receptors
title_full Animal Models for the Investigation of P2X7 Receptors
title_fullStr Animal Models for the Investigation of P2X7 Receptors
title_full_unstemmed Animal Models for the Investigation of P2X7 Receptors
title_short Animal Models for the Investigation of P2X7 Receptors
title_sort animal models for the investigation of p2x7 receptors
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10179175/
https://www.ncbi.nlm.nih.gov/pubmed/37175933
http://dx.doi.org/10.3390/ijms24098225
work_keys_str_mv AT sluyterronald animalmodelsfortheinvestigationofp2x7receptors
AT adriouchsahil animalmodelsfortheinvestigationofp2x7receptors
AT fullerstephenj animalmodelsfortheinvestigationofp2x7receptors
AT nickeannette animalmodelsfortheinvestigationofp2x7receptors
AT sophocleousreecea animalmodelsfortheinvestigationofp2x7receptors
AT watsondebbie animalmodelsfortheinvestigationofp2x7receptors