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Animal Models for the Investigation of P2X7 Receptors
The P2X7 receptor is a trimeric ligand-gated cation channel activated by extracellular adenosine 5′-triphosphate. The study of animals has greatly advanced the investigation of P2X7 and helped to establish the numerous physiological and pathophysiological roles of this receptor in human health and d...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10179175/ https://www.ncbi.nlm.nih.gov/pubmed/37175933 http://dx.doi.org/10.3390/ijms24098225 |
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author | Sluyter, Ronald Adriouch, Sahil Fuller, Stephen J. Nicke, Annette Sophocleous, Reece A. Watson, Debbie |
author_facet | Sluyter, Ronald Adriouch, Sahil Fuller, Stephen J. Nicke, Annette Sophocleous, Reece A. Watson, Debbie |
author_sort | Sluyter, Ronald |
collection | PubMed |
description | The P2X7 receptor is a trimeric ligand-gated cation channel activated by extracellular adenosine 5′-triphosphate. The study of animals has greatly advanced the investigation of P2X7 and helped to establish the numerous physiological and pathophysiological roles of this receptor in human health and disease. Following a short overview of the P2X7 distribution, roles and functional properties, this article discusses how animal models have contributed to the generation of P2X7-specific antibodies and nanobodies (including biologics), recombinant receptors and radioligands to study P2X7 as well as to the pharmacokinetic testing of P2X7 antagonists. This article then outlines how mouse and rat models have been used to study P2X7. These sections include discussions on preclinical disease models, polymorphic P2X7 variants, P2X7 knockout mice (including bone marrow chimeras and conditional knockouts), P2X7 reporter mice, humanized P2X7 mice and P2X7 knockout rats. Finally, this article reviews the limited number of studies involving guinea pigs, rabbits, monkeys (rhesus macaques), dogs, cats, zebrafish, and other fish species (seabream, ayu sweetfish, rainbow trout and Japanese flounder) to study P2X7. |
format | Online Article Text |
id | pubmed-10179175 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101791752023-05-13 Animal Models for the Investigation of P2X7 Receptors Sluyter, Ronald Adriouch, Sahil Fuller, Stephen J. Nicke, Annette Sophocleous, Reece A. Watson, Debbie Int J Mol Sci Review The P2X7 receptor is a trimeric ligand-gated cation channel activated by extracellular adenosine 5′-triphosphate. The study of animals has greatly advanced the investigation of P2X7 and helped to establish the numerous physiological and pathophysiological roles of this receptor in human health and disease. Following a short overview of the P2X7 distribution, roles and functional properties, this article discusses how animal models have contributed to the generation of P2X7-specific antibodies and nanobodies (including biologics), recombinant receptors and radioligands to study P2X7 as well as to the pharmacokinetic testing of P2X7 antagonists. This article then outlines how mouse and rat models have been used to study P2X7. These sections include discussions on preclinical disease models, polymorphic P2X7 variants, P2X7 knockout mice (including bone marrow chimeras and conditional knockouts), P2X7 reporter mice, humanized P2X7 mice and P2X7 knockout rats. Finally, this article reviews the limited number of studies involving guinea pigs, rabbits, monkeys (rhesus macaques), dogs, cats, zebrafish, and other fish species (seabream, ayu sweetfish, rainbow trout and Japanese flounder) to study P2X7. MDPI 2023-05-04 /pmc/articles/PMC10179175/ /pubmed/37175933 http://dx.doi.org/10.3390/ijms24098225 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Sluyter, Ronald Adriouch, Sahil Fuller, Stephen J. Nicke, Annette Sophocleous, Reece A. Watson, Debbie Animal Models for the Investigation of P2X7 Receptors |
title | Animal Models for the Investigation of P2X7 Receptors |
title_full | Animal Models for the Investigation of P2X7 Receptors |
title_fullStr | Animal Models for the Investigation of P2X7 Receptors |
title_full_unstemmed | Animal Models for the Investigation of P2X7 Receptors |
title_short | Animal Models for the Investigation of P2X7 Receptors |
title_sort | animal models for the investigation of p2x7 receptors |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10179175/ https://www.ncbi.nlm.nih.gov/pubmed/37175933 http://dx.doi.org/10.3390/ijms24098225 |
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