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Applications of the Whole-Cell System in the Efficient Biosynthesis of Heme

Heme has a variety of functions, from electronic reactions to binding gases, which makes it useful in medical treatments, dietary supplements, and food processing. In recent years, whole-cell system-based heme biosynthesis methods have been continuously explored and optimized as an alternative to th...

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Detalles Bibliográficos
Autores principales: Su, Hongfei, Chen, Xiaolin, Chen, Shijing, Guo, Mingzhang, Liu, Huilin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10179345/
https://www.ncbi.nlm.nih.gov/pubmed/37176091
http://dx.doi.org/10.3390/ijms24098384
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author Su, Hongfei
Chen, Xiaolin
Chen, Shijing
Guo, Mingzhang
Liu, Huilin
author_facet Su, Hongfei
Chen, Xiaolin
Chen, Shijing
Guo, Mingzhang
Liu, Huilin
author_sort Su, Hongfei
collection PubMed
description Heme has a variety of functions, from electronic reactions to binding gases, which makes it useful in medical treatments, dietary supplements, and food processing. In recent years, whole-cell system-based heme biosynthesis methods have been continuously explored and optimized as an alternative to the low-yield, lasting, and adverse ecological environment of chemical synthesis methods. This method relies on two biosynthetic pathways of microbial precursor 5-aminolevulinic acid (C4, C5) and three known downstream biosynthetic pathways of heme. This paper reviews the genetic and metabolic engineering strategies for heme production in recent years by optimizing culture conditions and techniques from different microorganisms. Specifically, we summarized and analyzed the possibility of using biosensors to explore new strategies for the biosynthesis of heme from the perspective of synthetic biology, providing a new direction for future exploration.
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spelling pubmed-101793452023-05-13 Applications of the Whole-Cell System in the Efficient Biosynthesis of Heme Su, Hongfei Chen, Xiaolin Chen, Shijing Guo, Mingzhang Liu, Huilin Int J Mol Sci Review Heme has a variety of functions, from electronic reactions to binding gases, which makes it useful in medical treatments, dietary supplements, and food processing. In recent years, whole-cell system-based heme biosynthesis methods have been continuously explored and optimized as an alternative to the low-yield, lasting, and adverse ecological environment of chemical synthesis methods. This method relies on two biosynthetic pathways of microbial precursor 5-aminolevulinic acid (C4, C5) and three known downstream biosynthetic pathways of heme. This paper reviews the genetic and metabolic engineering strategies for heme production in recent years by optimizing culture conditions and techniques from different microorganisms. Specifically, we summarized and analyzed the possibility of using biosensors to explore new strategies for the biosynthesis of heme from the perspective of synthetic biology, providing a new direction for future exploration. MDPI 2023-05-07 /pmc/articles/PMC10179345/ /pubmed/37176091 http://dx.doi.org/10.3390/ijms24098384 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Su, Hongfei
Chen, Xiaolin
Chen, Shijing
Guo, Mingzhang
Liu, Huilin
Applications of the Whole-Cell System in the Efficient Biosynthesis of Heme
title Applications of the Whole-Cell System in the Efficient Biosynthesis of Heme
title_full Applications of the Whole-Cell System in the Efficient Biosynthesis of Heme
title_fullStr Applications of the Whole-Cell System in the Efficient Biosynthesis of Heme
title_full_unstemmed Applications of the Whole-Cell System in the Efficient Biosynthesis of Heme
title_short Applications of the Whole-Cell System in the Efficient Biosynthesis of Heme
title_sort applications of the whole-cell system in the efficient biosynthesis of heme
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10179345/
https://www.ncbi.nlm.nih.gov/pubmed/37176091
http://dx.doi.org/10.3390/ijms24098384
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