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Applications of the Whole-Cell System in the Efficient Biosynthesis of Heme
Heme has a variety of functions, from electronic reactions to binding gases, which makes it useful in medical treatments, dietary supplements, and food processing. In recent years, whole-cell system-based heme biosynthesis methods have been continuously explored and optimized as an alternative to th...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10179345/ https://www.ncbi.nlm.nih.gov/pubmed/37176091 http://dx.doi.org/10.3390/ijms24098384 |
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author | Su, Hongfei Chen, Xiaolin Chen, Shijing Guo, Mingzhang Liu, Huilin |
author_facet | Su, Hongfei Chen, Xiaolin Chen, Shijing Guo, Mingzhang Liu, Huilin |
author_sort | Su, Hongfei |
collection | PubMed |
description | Heme has a variety of functions, from electronic reactions to binding gases, which makes it useful in medical treatments, dietary supplements, and food processing. In recent years, whole-cell system-based heme biosynthesis methods have been continuously explored and optimized as an alternative to the low-yield, lasting, and adverse ecological environment of chemical synthesis methods. This method relies on two biosynthetic pathways of microbial precursor 5-aminolevulinic acid (C4, C5) and three known downstream biosynthetic pathways of heme. This paper reviews the genetic and metabolic engineering strategies for heme production in recent years by optimizing culture conditions and techniques from different microorganisms. Specifically, we summarized and analyzed the possibility of using biosensors to explore new strategies for the biosynthesis of heme from the perspective of synthetic biology, providing a new direction for future exploration. |
format | Online Article Text |
id | pubmed-10179345 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101793452023-05-13 Applications of the Whole-Cell System in the Efficient Biosynthesis of Heme Su, Hongfei Chen, Xiaolin Chen, Shijing Guo, Mingzhang Liu, Huilin Int J Mol Sci Review Heme has a variety of functions, from electronic reactions to binding gases, which makes it useful in medical treatments, dietary supplements, and food processing. In recent years, whole-cell system-based heme biosynthesis methods have been continuously explored and optimized as an alternative to the low-yield, lasting, and adverse ecological environment of chemical synthesis methods. This method relies on two biosynthetic pathways of microbial precursor 5-aminolevulinic acid (C4, C5) and three known downstream biosynthetic pathways of heme. This paper reviews the genetic and metabolic engineering strategies for heme production in recent years by optimizing culture conditions and techniques from different microorganisms. Specifically, we summarized and analyzed the possibility of using biosensors to explore new strategies for the biosynthesis of heme from the perspective of synthetic biology, providing a new direction for future exploration. MDPI 2023-05-07 /pmc/articles/PMC10179345/ /pubmed/37176091 http://dx.doi.org/10.3390/ijms24098384 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Su, Hongfei Chen, Xiaolin Chen, Shijing Guo, Mingzhang Liu, Huilin Applications of the Whole-Cell System in the Efficient Biosynthesis of Heme |
title | Applications of the Whole-Cell System in the Efficient Biosynthesis of Heme |
title_full | Applications of the Whole-Cell System in the Efficient Biosynthesis of Heme |
title_fullStr | Applications of the Whole-Cell System in the Efficient Biosynthesis of Heme |
title_full_unstemmed | Applications of the Whole-Cell System in the Efficient Biosynthesis of Heme |
title_short | Applications of the Whole-Cell System in the Efficient Biosynthesis of Heme |
title_sort | applications of the whole-cell system in the efficient biosynthesis of heme |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10179345/ https://www.ncbi.nlm.nih.gov/pubmed/37176091 http://dx.doi.org/10.3390/ijms24098384 |
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