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A Novel Localization of METTL7A in Bergmann Glial Cells in Human Cerebellum
Methyltransferase-like protein 7A (METTL7A) is a member of the METTL family of methyltransferases.Little information is available regarding the cellular expression of METTL7A in the brain. METTL7A is commonly located in the endoplasmic reticulum and to a lesser extent, in the lipid droplets of some...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10179429/ https://www.ncbi.nlm.nih.gov/pubmed/37176112 http://dx.doi.org/10.3390/ijms24098405 |
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author | Vera-Montecinos, América Galiano-Landeira, Jordi Roldán, Mònica Vidal-Domènech, Francisco Claro, Enrique Ramos, Belén |
author_facet | Vera-Montecinos, América Galiano-Landeira, Jordi Roldán, Mònica Vidal-Domènech, Francisco Claro, Enrique Ramos, Belén |
author_sort | Vera-Montecinos, América |
collection | PubMed |
description | Methyltransferase-like protein 7A (METTL7A) is a member of the METTL family of methyltransferases.Little information is available regarding the cellular expression of METTL7A in the brain. METTL7A is commonly located in the endoplasmic reticulum and to a lesser extent, in the lipid droplets of some cells. Several studies have reported altered protein and RNA levels in different brain areas in schizophrenia. One of these studies found reduced protein levels of METTL7A in the cerebellar cortex in schizophrenia and stress murine models. Since there is limited information in the literature about METTL7A, we characterized its cellular and subcellular localizations in the human cerebellum using immunohistochemical analysis with laser confocal microscopy. Our study reveals a novel METTL7A localization in GFAP-positive cells, with higher expression in the end-feet of the Bergmann glia, which participate in the cerebrospinal fluid–brain parenchyma barrier. Further 3D reconstruction image analysis showed that METTL7A was expressed in the contacts between the Bergmann glia and Purkinje neurons. METTL7A was also detected in lipid droplets in some cells in the white matter. The localization of METTL7A in the human cerebellar glia limitans could suggest a putative role in maintaining the cerebellar parenchyma homeostasis and in the regulation of internal cerebellar circuits by modulating the synaptic activity of Purkinje neurons. |
format | Online Article Text |
id | pubmed-10179429 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101794292023-05-13 A Novel Localization of METTL7A in Bergmann Glial Cells in Human Cerebellum Vera-Montecinos, América Galiano-Landeira, Jordi Roldán, Mònica Vidal-Domènech, Francisco Claro, Enrique Ramos, Belén Int J Mol Sci Article Methyltransferase-like protein 7A (METTL7A) is a member of the METTL family of methyltransferases.Little information is available regarding the cellular expression of METTL7A in the brain. METTL7A is commonly located in the endoplasmic reticulum and to a lesser extent, in the lipid droplets of some cells. Several studies have reported altered protein and RNA levels in different brain areas in schizophrenia. One of these studies found reduced protein levels of METTL7A in the cerebellar cortex in schizophrenia and stress murine models. Since there is limited information in the literature about METTL7A, we characterized its cellular and subcellular localizations in the human cerebellum using immunohistochemical analysis with laser confocal microscopy. Our study reveals a novel METTL7A localization in GFAP-positive cells, with higher expression in the end-feet of the Bergmann glia, which participate in the cerebrospinal fluid–brain parenchyma barrier. Further 3D reconstruction image analysis showed that METTL7A was expressed in the contacts between the Bergmann glia and Purkinje neurons. METTL7A was also detected in lipid droplets in some cells in the white matter. The localization of METTL7A in the human cerebellar glia limitans could suggest a putative role in maintaining the cerebellar parenchyma homeostasis and in the regulation of internal cerebellar circuits by modulating the synaptic activity of Purkinje neurons. MDPI 2023-05-07 /pmc/articles/PMC10179429/ /pubmed/37176112 http://dx.doi.org/10.3390/ijms24098405 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Vera-Montecinos, América Galiano-Landeira, Jordi Roldán, Mònica Vidal-Domènech, Francisco Claro, Enrique Ramos, Belén A Novel Localization of METTL7A in Bergmann Glial Cells in Human Cerebellum |
title | A Novel Localization of METTL7A in Bergmann Glial Cells in Human Cerebellum |
title_full | A Novel Localization of METTL7A in Bergmann Glial Cells in Human Cerebellum |
title_fullStr | A Novel Localization of METTL7A in Bergmann Glial Cells in Human Cerebellum |
title_full_unstemmed | A Novel Localization of METTL7A in Bergmann Glial Cells in Human Cerebellum |
title_short | A Novel Localization of METTL7A in Bergmann Glial Cells in Human Cerebellum |
title_sort | novel localization of mettl7a in bergmann glial cells in human cerebellum |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10179429/ https://www.ncbi.nlm.nih.gov/pubmed/37176112 http://dx.doi.org/10.3390/ijms24098405 |
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