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Lactoferrin Modulates Induction of Transcription Factor c-Fos in Neuronal Cultures
Lactoferrin (Lf) is a multifunctional protein from the transferrin family. Of particular interest is the ability of Lf to affect a wide range of neuronal processes by modulating the expression of genes involved in long-term neuroplasticity. The expression of the immediate early gene c-fos that is ra...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10179438/ https://www.ncbi.nlm.nih.gov/pubmed/37176079 http://dx.doi.org/10.3390/ijms24098373 |
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author | Kopaeva, Marina Yu. Azieva, Asya M. Cherepov, Anton B. Zarayskaya, Irina Yu. |
author_facet | Kopaeva, Marina Yu. Azieva, Asya M. Cherepov, Anton B. Zarayskaya, Irina Yu. |
author_sort | Kopaeva, Marina Yu. |
collection | PubMed |
description | Lactoferrin (Lf) is a multifunctional protein from the transferrin family. Of particular interest is the ability of Lf to affect a wide range of neuronal processes by modulating the expression of genes involved in long-term neuroplasticity. The expression of the immediate early gene c-fos that is rapidly activated in response to external influences, and its product, transcription factor c-Fos, is widely used as a marker of long-term neuronal plasticity. The present study aims to examine the effect of human Lf on the induction of transcription factor c-Fos in the primary mouse neuronal cultures after stimulation and to determine the cellular localization of human Lf and its colocalization with induced c-Fos protein. Primary dissociated cultures of hippocampal cells were obtained from the brains of newborn C57BL/6 mice (P0-P1). On day 7 of culturing, human Lf was added to the medium. After 24 h (day 8 in culture), c-Fos protein was induced in cells by triple application of 50 mM KCl. c-Fos content was analyzed using the immunofluorescent method 2 h after stimulation. Stimulation promoted exogenous Lf translocation into the nuclei of cultured neuronal cells, which correlated with increased induction of transcription factor c-Fos and was accompanied by nuclear colocalization of these proteins. These results attest to the potential of Lf as a modulator of neuronal processes and open up new prospects in studying the mechanisms of the regulatory effects of lactoferrin on cell function. |
format | Online Article Text |
id | pubmed-10179438 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101794382023-05-13 Lactoferrin Modulates Induction of Transcription Factor c-Fos in Neuronal Cultures Kopaeva, Marina Yu. Azieva, Asya M. Cherepov, Anton B. Zarayskaya, Irina Yu. Int J Mol Sci Article Lactoferrin (Lf) is a multifunctional protein from the transferrin family. Of particular interest is the ability of Lf to affect a wide range of neuronal processes by modulating the expression of genes involved in long-term neuroplasticity. The expression of the immediate early gene c-fos that is rapidly activated in response to external influences, and its product, transcription factor c-Fos, is widely used as a marker of long-term neuronal plasticity. The present study aims to examine the effect of human Lf on the induction of transcription factor c-Fos in the primary mouse neuronal cultures after stimulation and to determine the cellular localization of human Lf and its colocalization with induced c-Fos protein. Primary dissociated cultures of hippocampal cells were obtained from the brains of newborn C57BL/6 mice (P0-P1). On day 7 of culturing, human Lf was added to the medium. After 24 h (day 8 in culture), c-Fos protein was induced in cells by triple application of 50 mM KCl. c-Fos content was analyzed using the immunofluorescent method 2 h after stimulation. Stimulation promoted exogenous Lf translocation into the nuclei of cultured neuronal cells, which correlated with increased induction of transcription factor c-Fos and was accompanied by nuclear colocalization of these proteins. These results attest to the potential of Lf as a modulator of neuronal processes and open up new prospects in studying the mechanisms of the regulatory effects of lactoferrin on cell function. MDPI 2023-05-06 /pmc/articles/PMC10179438/ /pubmed/37176079 http://dx.doi.org/10.3390/ijms24098373 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kopaeva, Marina Yu. Azieva, Asya M. Cherepov, Anton B. Zarayskaya, Irina Yu. Lactoferrin Modulates Induction of Transcription Factor c-Fos in Neuronal Cultures |
title | Lactoferrin Modulates Induction of Transcription Factor c-Fos in Neuronal Cultures |
title_full | Lactoferrin Modulates Induction of Transcription Factor c-Fos in Neuronal Cultures |
title_fullStr | Lactoferrin Modulates Induction of Transcription Factor c-Fos in Neuronal Cultures |
title_full_unstemmed | Lactoferrin Modulates Induction of Transcription Factor c-Fos in Neuronal Cultures |
title_short | Lactoferrin Modulates Induction of Transcription Factor c-Fos in Neuronal Cultures |
title_sort | lactoferrin modulates induction of transcription factor c-fos in neuronal cultures |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10179438/ https://www.ncbi.nlm.nih.gov/pubmed/37176079 http://dx.doi.org/10.3390/ijms24098373 |
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