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Inflammatory, Oxidative Stress and Small Cellular Particle Response in HUVEC Induced by Debris from Endoprosthesis Processing

We studied inflammatory and oxidative stress-related parameters and cytotoxic response of human umbilical vein endothelial cells (HUVEC) to a 24 h treatment with milled particles simulating debris involved in sandblasting of orthopedic implants (OI). We used different abrasives (corundum—(Al(2)O(3))...

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Autores principales: Jan, Zala, Hočevar, Matej, Kononenko, Veno, Michelini, Sara, Repar, Neža, Caf, Maja, Kocjančič, Boštjan, Dolinar, Drago, Kralj, Slavko, Makovec, Darko, Iglič, Aleš, Drobne, Damjana, Jenko, Monika, Kralj-Iglič, Veronika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10179554/
https://www.ncbi.nlm.nih.gov/pubmed/37176169
http://dx.doi.org/10.3390/ma16093287
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author Jan, Zala
Hočevar, Matej
Kononenko, Veno
Michelini, Sara
Repar, Neža
Caf, Maja
Kocjančič, Boštjan
Dolinar, Drago
Kralj, Slavko
Makovec, Darko
Iglič, Aleš
Drobne, Damjana
Jenko, Monika
Kralj-Iglič, Veronika
author_facet Jan, Zala
Hočevar, Matej
Kononenko, Veno
Michelini, Sara
Repar, Neža
Caf, Maja
Kocjančič, Boštjan
Dolinar, Drago
Kralj, Slavko
Makovec, Darko
Iglič, Aleš
Drobne, Damjana
Jenko, Monika
Kralj-Iglič, Veronika
author_sort Jan, Zala
collection PubMed
description We studied inflammatory and oxidative stress-related parameters and cytotoxic response of human umbilical vein endothelial cells (HUVEC) to a 24 h treatment with milled particles simulating debris involved in sandblasting of orthopedic implants (OI). We used different abrasives (corundum—(Al(2)O(3)), used corundum retrieved from removed OI (u. Al(2)O(3)), and zirconia/silica composite (ZrO(2)/SiO(2))). Morphological changes were observed by scanning electron microscopy (SEM). Concentration of Interleukins IL-6 and IL-1β and Tumor Necrosis Factor α (TNF)-α was assessed by enzyme-linked immunosorbent assay (ELISA). Activity of Cholinesterase (ChE) and Glutathione S-transferase (GST) was measured by spectrophotometry. Reactive oxygen species (ROS), lipid droplets (LD) and apoptosis were measured by flow cytometry (FCM). Detachment of the cells from glass and budding of the cell membrane did not differ in the treated and untreated control cells. Increased concentration of IL-1β and of IL-6 was found after treatment with all tested particle types, indicating inflammatory response of the treated cells. Increased ChE activity was found after treatment with u. Al(2)O(3) and ZrO(2)/SiO(2). Increased GST activity was found after treatment with ZrO(2)/SiO(2). Increased LD quantity but not ROS quantity was found after treatment with u. Al(2)O(3). No cytotoxicity was detected after treatment with u. Al(2)O(3). The tested materials in concentrations added to in vitro cell lines were found non-toxic but bioactive and therefore prone to induce a response of the human body to OI.
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spelling pubmed-101795542023-05-13 Inflammatory, Oxidative Stress and Small Cellular Particle Response in HUVEC Induced by Debris from Endoprosthesis Processing Jan, Zala Hočevar, Matej Kononenko, Veno Michelini, Sara Repar, Neža Caf, Maja Kocjančič, Boštjan Dolinar, Drago Kralj, Slavko Makovec, Darko Iglič, Aleš Drobne, Damjana Jenko, Monika Kralj-Iglič, Veronika Materials (Basel) Article We studied inflammatory and oxidative stress-related parameters and cytotoxic response of human umbilical vein endothelial cells (HUVEC) to a 24 h treatment with milled particles simulating debris involved in sandblasting of orthopedic implants (OI). We used different abrasives (corundum—(Al(2)O(3)), used corundum retrieved from removed OI (u. Al(2)O(3)), and zirconia/silica composite (ZrO(2)/SiO(2))). Morphological changes were observed by scanning electron microscopy (SEM). Concentration of Interleukins IL-6 and IL-1β and Tumor Necrosis Factor α (TNF)-α was assessed by enzyme-linked immunosorbent assay (ELISA). Activity of Cholinesterase (ChE) and Glutathione S-transferase (GST) was measured by spectrophotometry. Reactive oxygen species (ROS), lipid droplets (LD) and apoptosis were measured by flow cytometry (FCM). Detachment of the cells from glass and budding of the cell membrane did not differ in the treated and untreated control cells. Increased concentration of IL-1β and of IL-6 was found after treatment with all tested particle types, indicating inflammatory response of the treated cells. Increased ChE activity was found after treatment with u. Al(2)O(3) and ZrO(2)/SiO(2). Increased GST activity was found after treatment with ZrO(2)/SiO(2). Increased LD quantity but not ROS quantity was found after treatment with u. Al(2)O(3). No cytotoxicity was detected after treatment with u. Al(2)O(3). The tested materials in concentrations added to in vitro cell lines were found non-toxic but bioactive and therefore prone to induce a response of the human body to OI. MDPI 2023-04-22 /pmc/articles/PMC10179554/ /pubmed/37176169 http://dx.doi.org/10.3390/ma16093287 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jan, Zala
Hočevar, Matej
Kononenko, Veno
Michelini, Sara
Repar, Neža
Caf, Maja
Kocjančič, Boštjan
Dolinar, Drago
Kralj, Slavko
Makovec, Darko
Iglič, Aleš
Drobne, Damjana
Jenko, Monika
Kralj-Iglič, Veronika
Inflammatory, Oxidative Stress and Small Cellular Particle Response in HUVEC Induced by Debris from Endoprosthesis Processing
title Inflammatory, Oxidative Stress and Small Cellular Particle Response in HUVEC Induced by Debris from Endoprosthesis Processing
title_full Inflammatory, Oxidative Stress and Small Cellular Particle Response in HUVEC Induced by Debris from Endoprosthesis Processing
title_fullStr Inflammatory, Oxidative Stress and Small Cellular Particle Response in HUVEC Induced by Debris from Endoprosthesis Processing
title_full_unstemmed Inflammatory, Oxidative Stress and Small Cellular Particle Response in HUVEC Induced by Debris from Endoprosthesis Processing
title_short Inflammatory, Oxidative Stress and Small Cellular Particle Response in HUVEC Induced by Debris from Endoprosthesis Processing
title_sort inflammatory, oxidative stress and small cellular particle response in huvec induced by debris from endoprosthesis processing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10179554/
https://www.ncbi.nlm.nih.gov/pubmed/37176169
http://dx.doi.org/10.3390/ma16093287
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