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Extraction of Isoflavones, Alpha-Hydroxy Acids, and Allantoin from Soybean Leaves—Optimization by a Mixture Design of the Experimental Method

Soybeans are commonly known as a valuable source of biologically active compounds including isoflavones as well as allantoin and alpha-hydroxy acids. Since these compounds exhibit skin therapeutic effects, they are widely used in the cosmetic and pharmaceutical industries. The presented paper shows...

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Autores principales: Dresler, Sławomir, Strzemski, Maciej, Baczewska, Izabela, Koselski, Mateusz, Hassanpouraghdam, Mohammad Bagher, Szczepanek, Dariusz, Sowa, Ireneusz, Wójciak, Magdalena, Hanaka, Agnieszka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10179801/
https://www.ncbi.nlm.nih.gov/pubmed/37175385
http://dx.doi.org/10.3390/molecules28093963
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author Dresler, Sławomir
Strzemski, Maciej
Baczewska, Izabela
Koselski, Mateusz
Hassanpouraghdam, Mohammad Bagher
Szczepanek, Dariusz
Sowa, Ireneusz
Wójciak, Magdalena
Hanaka, Agnieszka
author_facet Dresler, Sławomir
Strzemski, Maciej
Baczewska, Izabela
Koselski, Mateusz
Hassanpouraghdam, Mohammad Bagher
Szczepanek, Dariusz
Sowa, Ireneusz
Wójciak, Magdalena
Hanaka, Agnieszka
author_sort Dresler, Sławomir
collection PubMed
description Soybeans are commonly known as a valuable source of biologically active compounds including isoflavones as well as allantoin and alpha-hydroxy acids. Since these compounds exhibit skin therapeutic effects, they are widely used in the cosmetic and pharmaceutical industries. The presented paper shows the optimization of three solvent systems (ethanol, water, and 1,3-propanediol) to increase the extraction efficiency of isoflavones (daidzin, genistin, 6″-O-malonyldaidzin, 6″-O-malonylglycitin, 6″-O-malonylgenistin), allantoin, and alpha-hydroxy acids (citric acid, malic acid) from soybean leaves. A simplex centroid mixture design for three solvents with interior points was applied for the experimental plan creation. Based on the obtained results of metabolite extraction yield in relation to solvent composition, polynomial regression models were developed. All models were significant, with predicted R-squared values between 0.77 and 0.99, while in all cases the model’s lack of fit was not significant. The optimal mixture composition enabling the maximization of extraction efficiency was as follows: 32.9% ethanol, 53.9% water, and 13.3% propanediol (v/v/v). Such a mixture composition provided the extraction of 99%, 91%, 100%, 92%, 99%, 70%, 92%, and 69% of daidzin, genistin, 6″-O-malonyldaidzin, 6″-O-malonylglycitin, 6″-O-malonylgenistin, allantoin, citric acid, and malic acid, respectively. The solvent mixture composition developed provides a good extraction efficiency of the metabolites from soybean leaves and high antioxidant properties.
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spelling pubmed-101798012023-05-13 Extraction of Isoflavones, Alpha-Hydroxy Acids, and Allantoin from Soybean Leaves—Optimization by a Mixture Design of the Experimental Method Dresler, Sławomir Strzemski, Maciej Baczewska, Izabela Koselski, Mateusz Hassanpouraghdam, Mohammad Bagher Szczepanek, Dariusz Sowa, Ireneusz Wójciak, Magdalena Hanaka, Agnieszka Molecules Article Soybeans are commonly known as a valuable source of biologically active compounds including isoflavones as well as allantoin and alpha-hydroxy acids. Since these compounds exhibit skin therapeutic effects, they are widely used in the cosmetic and pharmaceutical industries. The presented paper shows the optimization of three solvent systems (ethanol, water, and 1,3-propanediol) to increase the extraction efficiency of isoflavones (daidzin, genistin, 6″-O-malonyldaidzin, 6″-O-malonylglycitin, 6″-O-malonylgenistin), allantoin, and alpha-hydroxy acids (citric acid, malic acid) from soybean leaves. A simplex centroid mixture design for three solvents with interior points was applied for the experimental plan creation. Based on the obtained results of metabolite extraction yield in relation to solvent composition, polynomial regression models were developed. All models were significant, with predicted R-squared values between 0.77 and 0.99, while in all cases the model’s lack of fit was not significant. The optimal mixture composition enabling the maximization of extraction efficiency was as follows: 32.9% ethanol, 53.9% water, and 13.3% propanediol (v/v/v). Such a mixture composition provided the extraction of 99%, 91%, 100%, 92%, 99%, 70%, 92%, and 69% of daidzin, genistin, 6″-O-malonyldaidzin, 6″-O-malonylglycitin, 6″-O-malonylgenistin, allantoin, citric acid, and malic acid, respectively. The solvent mixture composition developed provides a good extraction efficiency of the metabolites from soybean leaves and high antioxidant properties. MDPI 2023-05-08 /pmc/articles/PMC10179801/ /pubmed/37175385 http://dx.doi.org/10.3390/molecules28093963 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dresler, Sławomir
Strzemski, Maciej
Baczewska, Izabela
Koselski, Mateusz
Hassanpouraghdam, Mohammad Bagher
Szczepanek, Dariusz
Sowa, Ireneusz
Wójciak, Magdalena
Hanaka, Agnieszka
Extraction of Isoflavones, Alpha-Hydroxy Acids, and Allantoin from Soybean Leaves—Optimization by a Mixture Design of the Experimental Method
title Extraction of Isoflavones, Alpha-Hydroxy Acids, and Allantoin from Soybean Leaves—Optimization by a Mixture Design of the Experimental Method
title_full Extraction of Isoflavones, Alpha-Hydroxy Acids, and Allantoin from Soybean Leaves—Optimization by a Mixture Design of the Experimental Method
title_fullStr Extraction of Isoflavones, Alpha-Hydroxy Acids, and Allantoin from Soybean Leaves—Optimization by a Mixture Design of the Experimental Method
title_full_unstemmed Extraction of Isoflavones, Alpha-Hydroxy Acids, and Allantoin from Soybean Leaves—Optimization by a Mixture Design of the Experimental Method
title_short Extraction of Isoflavones, Alpha-Hydroxy Acids, and Allantoin from Soybean Leaves—Optimization by a Mixture Design of the Experimental Method
title_sort extraction of isoflavones, alpha-hydroxy acids, and allantoin from soybean leaves—optimization by a mixture design of the experimental method
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10179801/
https://www.ncbi.nlm.nih.gov/pubmed/37175385
http://dx.doi.org/10.3390/molecules28093963
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