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Novel Hyperspectral Analysis of Thin-Layer Chromatographic Plates—An Application to Fingerprinting of 70 Polish Grasses

The advantages of hyperspectral imaging in videodensitometry are presented and discussed with the example of extracts from 70 Polish grasses. An inexpensive microscope camera was modified to cover the infrared spectrum range, and then 11 combinations of illumination (254 nm, 366 nm, white light), to...

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Autores principales: Wróbel-Szkolak, Joanna, Cwener, Anna, Komsta, Łukasz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10179984/
https://www.ncbi.nlm.nih.gov/pubmed/37175155
http://dx.doi.org/10.3390/molecules28093745
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author Wróbel-Szkolak, Joanna
Cwener, Anna
Komsta, Łukasz
author_facet Wróbel-Szkolak, Joanna
Cwener, Anna
Komsta, Łukasz
author_sort Wróbel-Szkolak, Joanna
collection PubMed
description The advantages of hyperspectral imaging in videodensitometry are presented and discussed with the example of extracts from 70 Polish grasses. An inexpensive microscope camera was modified to cover the infrared spectrum range, and then 11 combinations of illumination (254 nm, 366 nm, white light), together with various filters (no filter, IRCut, UV, cobalt glass, IR pass), were used to register RGB HDR images of the same plate. It was revealed that the resulting 33 channels of information could be compressed into 5–6 principal components and then visualized separately as grayscale images. We also propose a new approach called principal component artificial coloring of images (PCACI). It allows easy classification of chromatographic spots by presenting three PC components as RGB channels, providing vivid spots with artificial colors and visualizing six principal components on two color images. The infrared region brings additional information to the registered data, orthogonal to the other channels and not redundant with photos in the visible region. This is the first published attempt to use a hyperspectral camera in TLC and it can be clearly concluded that such an approach deserves routine use and further attention.
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spelling pubmed-101799842023-05-13 Novel Hyperspectral Analysis of Thin-Layer Chromatographic Plates—An Application to Fingerprinting of 70 Polish Grasses Wróbel-Szkolak, Joanna Cwener, Anna Komsta, Łukasz Molecules Article The advantages of hyperspectral imaging in videodensitometry are presented and discussed with the example of extracts from 70 Polish grasses. An inexpensive microscope camera was modified to cover the infrared spectrum range, and then 11 combinations of illumination (254 nm, 366 nm, white light), together with various filters (no filter, IRCut, UV, cobalt glass, IR pass), were used to register RGB HDR images of the same plate. It was revealed that the resulting 33 channels of information could be compressed into 5–6 principal components and then visualized separately as grayscale images. We also propose a new approach called principal component artificial coloring of images (PCACI). It allows easy classification of chromatographic spots by presenting three PC components as RGB channels, providing vivid spots with artificial colors and visualizing six principal components on two color images. The infrared region brings additional information to the registered data, orthogonal to the other channels and not redundant with photos in the visible region. This is the first published attempt to use a hyperspectral camera in TLC and it can be clearly concluded that such an approach deserves routine use and further attention. MDPI 2023-04-26 /pmc/articles/PMC10179984/ /pubmed/37175155 http://dx.doi.org/10.3390/molecules28093745 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wróbel-Szkolak, Joanna
Cwener, Anna
Komsta, Łukasz
Novel Hyperspectral Analysis of Thin-Layer Chromatographic Plates—An Application to Fingerprinting of 70 Polish Grasses
title Novel Hyperspectral Analysis of Thin-Layer Chromatographic Plates—An Application to Fingerprinting of 70 Polish Grasses
title_full Novel Hyperspectral Analysis of Thin-Layer Chromatographic Plates—An Application to Fingerprinting of 70 Polish Grasses
title_fullStr Novel Hyperspectral Analysis of Thin-Layer Chromatographic Plates—An Application to Fingerprinting of 70 Polish Grasses
title_full_unstemmed Novel Hyperspectral Analysis of Thin-Layer Chromatographic Plates—An Application to Fingerprinting of 70 Polish Grasses
title_short Novel Hyperspectral Analysis of Thin-Layer Chromatographic Plates—An Application to Fingerprinting of 70 Polish Grasses
title_sort novel hyperspectral analysis of thin-layer chromatographic plates—an application to fingerprinting of 70 polish grasses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10179984/
https://www.ncbi.nlm.nih.gov/pubmed/37175155
http://dx.doi.org/10.3390/molecules28093745
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