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Enantioselective Labeling of Zebrafish for D-Phenylalanine Based on Graphene-Based Nanoplatform

Enantioselective labeling of important bioactive molecules in complex biological environments by artificial receptors has drawn great interest. From both the slight difference of enantiomers’ physicochemical properties and inherently complexity in living organism point of view, it is still a contemp...

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Autores principales: He, Yuqing, Ye, Ziqi, Zhu, Fei, Qiu, Tianxiang, Dai, Xiyan, Xie, Yue, Zou, Shibiao, Dong, Qingjian, Zhang, Weiying, Ma, Junkai, Mao, Xiaowei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10180043/
https://www.ncbi.nlm.nih.gov/pubmed/37175110
http://dx.doi.org/10.3390/molecules28093700
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author He, Yuqing
Ye, Ziqi
Zhu, Fei
Qiu, Tianxiang
Dai, Xiyan
Xie, Yue
Zou, Shibiao
Dong, Qingjian
Zhang, Weiying
Ma, Junkai
Mao, Xiaowei
author_facet He, Yuqing
Ye, Ziqi
Zhu, Fei
Qiu, Tianxiang
Dai, Xiyan
Xie, Yue
Zou, Shibiao
Dong, Qingjian
Zhang, Weiying
Ma, Junkai
Mao, Xiaowei
author_sort He, Yuqing
collection PubMed
description Enantioselective labeling of important bioactive molecules in complex biological environments by artificial receptors has drawn great interest. From both the slight difference of enantiomers’ physicochemical properties and inherently complexity in living organism point of view, it is still a contemporary challenge for preparing practical chiral device that could be employed in the model animal due to diverse biological interference. Herein, we introduce γ-cyclodextrin onto graphene oxide for fabricating γ-cyclodextrin and graphene oxide assemblies, which provided an efficient nanoplatform for chiral labelling of D-phenylalanine with higher chiral discrimination ratio of K(D)/K(L) = 8.21. Significantly, the chiral fluorescence quenching effect of this γ-CD-GO nanoplatform for D-phenylalanine enantiomer in zebrafish was 7.0-fold higher than L-isomer, which exhibiting real promise for producing practical enantio-differentiating graphene-based systems in a complex biological sample.
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spelling pubmed-101800432023-05-13 Enantioselective Labeling of Zebrafish for D-Phenylalanine Based on Graphene-Based Nanoplatform He, Yuqing Ye, Ziqi Zhu, Fei Qiu, Tianxiang Dai, Xiyan Xie, Yue Zou, Shibiao Dong, Qingjian Zhang, Weiying Ma, Junkai Mao, Xiaowei Molecules Article Enantioselective labeling of important bioactive molecules in complex biological environments by artificial receptors has drawn great interest. From both the slight difference of enantiomers’ physicochemical properties and inherently complexity in living organism point of view, it is still a contemporary challenge for preparing practical chiral device that could be employed in the model animal due to diverse biological interference. Herein, we introduce γ-cyclodextrin onto graphene oxide for fabricating γ-cyclodextrin and graphene oxide assemblies, which provided an efficient nanoplatform for chiral labelling of D-phenylalanine with higher chiral discrimination ratio of K(D)/K(L) = 8.21. Significantly, the chiral fluorescence quenching effect of this γ-CD-GO nanoplatform for D-phenylalanine enantiomer in zebrafish was 7.0-fold higher than L-isomer, which exhibiting real promise for producing practical enantio-differentiating graphene-based systems in a complex biological sample. MDPI 2023-04-25 /pmc/articles/PMC10180043/ /pubmed/37175110 http://dx.doi.org/10.3390/molecules28093700 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
He, Yuqing
Ye, Ziqi
Zhu, Fei
Qiu, Tianxiang
Dai, Xiyan
Xie, Yue
Zou, Shibiao
Dong, Qingjian
Zhang, Weiying
Ma, Junkai
Mao, Xiaowei
Enantioselective Labeling of Zebrafish for D-Phenylalanine Based on Graphene-Based Nanoplatform
title Enantioselective Labeling of Zebrafish for D-Phenylalanine Based on Graphene-Based Nanoplatform
title_full Enantioselective Labeling of Zebrafish for D-Phenylalanine Based on Graphene-Based Nanoplatform
title_fullStr Enantioselective Labeling of Zebrafish for D-Phenylalanine Based on Graphene-Based Nanoplatform
title_full_unstemmed Enantioselective Labeling of Zebrafish for D-Phenylalanine Based on Graphene-Based Nanoplatform
title_short Enantioselective Labeling of Zebrafish for D-Phenylalanine Based on Graphene-Based Nanoplatform
title_sort enantioselective labeling of zebrafish for d-phenylalanine based on graphene-based nanoplatform
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10180043/
https://www.ncbi.nlm.nih.gov/pubmed/37175110
http://dx.doi.org/10.3390/molecules28093700
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