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Chemical Characterisation, Antidiabetic, Antibacterial, and In Silico Studies for Different Extracts of Haloxylon stocksii (Boiss.) Benth: A Promising Halophyte

The objective of the study is to evaluate the chemical characterisation, and biological and in silico potential of Haloxylon stocksii (Boiss.) Benth, an important halophyte commonly used in traditional medicine. The research focuses on the roots and aerial parts of the plant and extracts them using...

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Autores principales: Rizvi, Syed Nabil Raza, Afzal, Samina, Khan, Kashif-ur-Rehman, Aati, Hanan Y., Rao, Huma, Ghalloo, Bilal Ahmad, Shahzad, Muhammad Nadeem, Khan, Duraiz Ahmed, Esatbeyoglu, Tuba, Korma, Sameh A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10180423/
https://www.ncbi.nlm.nih.gov/pubmed/37175255
http://dx.doi.org/10.3390/molecules28093847
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author Rizvi, Syed Nabil Raza
Afzal, Samina
Khan, Kashif-ur-Rehman
Aati, Hanan Y.
Rao, Huma
Ghalloo, Bilal Ahmad
Shahzad, Muhammad Nadeem
Khan, Duraiz Ahmed
Esatbeyoglu, Tuba
Korma, Sameh A.
author_facet Rizvi, Syed Nabil Raza
Afzal, Samina
Khan, Kashif-ur-Rehman
Aati, Hanan Y.
Rao, Huma
Ghalloo, Bilal Ahmad
Shahzad, Muhammad Nadeem
Khan, Duraiz Ahmed
Esatbeyoglu, Tuba
Korma, Sameh A.
author_sort Rizvi, Syed Nabil Raza
collection PubMed
description The objective of the study is to evaluate the chemical characterisation, and biological and in silico potential of Haloxylon stocksii (Boiss.) Benth, an important halophyte commonly used in traditional medicine. The research focuses on the roots and aerial parts of the plant and extracts them using two solvents: methanol and dichloromethane. Chemical characterisation of the extracts was carried out using total phenolic contents quantification, GC-MS analysis, and LC-MS screening. The results exhibited that the aerial parts of the plant have significantly higher total phenolic content than the roots. The GC-MS and LC-MS analysis of the plant extracts revealed the identification of 18 bioactive compounds in each. The biological evaluation was performed using antioxidant, antibacterial, and in vitro antidiabetic assays. The results exhibited that the aerial parts of the plant have higher antioxidant and in vitro antidiabetic activity than the roots. Additionally, the aerial parts of the plant were most effective against Gram-positive bacteria. Molecular docking was done to evaluate the binding affinity (BA) of the bioactive compounds characterised by GC-MS with diabetic enzymes used in the in vitro assay. The results showed that the BA of γ-sitosterol was better than that of acarbose, which is used as a standard in the in vitro assay. Overall, this study suggests that the extract from aerial parts of H. stocksii using methanol as a solvent have better potential as a new medicinal plant and can provide a new aspect to develop more potent medications. The research findings contribute to the scientific data of the medicinal properties of Haloxylon stocksii and provide a basis for further evaluation of its potential as a natural remedy.
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spelling pubmed-101804232023-05-13 Chemical Characterisation, Antidiabetic, Antibacterial, and In Silico Studies for Different Extracts of Haloxylon stocksii (Boiss.) Benth: A Promising Halophyte Rizvi, Syed Nabil Raza Afzal, Samina Khan, Kashif-ur-Rehman Aati, Hanan Y. Rao, Huma Ghalloo, Bilal Ahmad Shahzad, Muhammad Nadeem Khan, Duraiz Ahmed Esatbeyoglu, Tuba Korma, Sameh A. Molecules Article The objective of the study is to evaluate the chemical characterisation, and biological and in silico potential of Haloxylon stocksii (Boiss.) Benth, an important halophyte commonly used in traditional medicine. The research focuses on the roots and aerial parts of the plant and extracts them using two solvents: methanol and dichloromethane. Chemical characterisation of the extracts was carried out using total phenolic contents quantification, GC-MS analysis, and LC-MS screening. The results exhibited that the aerial parts of the plant have significantly higher total phenolic content than the roots. The GC-MS and LC-MS analysis of the plant extracts revealed the identification of 18 bioactive compounds in each. The biological evaluation was performed using antioxidant, antibacterial, and in vitro antidiabetic assays. The results exhibited that the aerial parts of the plant have higher antioxidant and in vitro antidiabetic activity than the roots. Additionally, the aerial parts of the plant were most effective against Gram-positive bacteria. Molecular docking was done to evaluate the binding affinity (BA) of the bioactive compounds characterised by GC-MS with diabetic enzymes used in the in vitro assay. The results showed that the BA of γ-sitosterol was better than that of acarbose, which is used as a standard in the in vitro assay. Overall, this study suggests that the extract from aerial parts of H. stocksii using methanol as a solvent have better potential as a new medicinal plant and can provide a new aspect to develop more potent medications. The research findings contribute to the scientific data of the medicinal properties of Haloxylon stocksii and provide a basis for further evaluation of its potential as a natural remedy. MDPI 2023-05-01 /pmc/articles/PMC10180423/ /pubmed/37175255 http://dx.doi.org/10.3390/molecules28093847 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rizvi, Syed Nabil Raza
Afzal, Samina
Khan, Kashif-ur-Rehman
Aati, Hanan Y.
Rao, Huma
Ghalloo, Bilal Ahmad
Shahzad, Muhammad Nadeem
Khan, Duraiz Ahmed
Esatbeyoglu, Tuba
Korma, Sameh A.
Chemical Characterisation, Antidiabetic, Antibacterial, and In Silico Studies for Different Extracts of Haloxylon stocksii (Boiss.) Benth: A Promising Halophyte
title Chemical Characterisation, Antidiabetic, Antibacterial, and In Silico Studies for Different Extracts of Haloxylon stocksii (Boiss.) Benth: A Promising Halophyte
title_full Chemical Characterisation, Antidiabetic, Antibacterial, and In Silico Studies for Different Extracts of Haloxylon stocksii (Boiss.) Benth: A Promising Halophyte
title_fullStr Chemical Characterisation, Antidiabetic, Antibacterial, and In Silico Studies for Different Extracts of Haloxylon stocksii (Boiss.) Benth: A Promising Halophyte
title_full_unstemmed Chemical Characterisation, Antidiabetic, Antibacterial, and In Silico Studies for Different Extracts of Haloxylon stocksii (Boiss.) Benth: A Promising Halophyte
title_short Chemical Characterisation, Antidiabetic, Antibacterial, and In Silico Studies for Different Extracts of Haloxylon stocksii (Boiss.) Benth: A Promising Halophyte
title_sort chemical characterisation, antidiabetic, antibacterial, and in silico studies for different extracts of haloxylon stocksii (boiss.) benth: a promising halophyte
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10180423/
https://www.ncbi.nlm.nih.gov/pubmed/37175255
http://dx.doi.org/10.3390/molecules28093847
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