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A Turn-On Quinazolinone-Based Fluorescence Probe for Selective Detection of Carbon Monoxide
Carbon monoxide (CO) is a toxic, hazardous gas that has a colorless and odorless nature. On the other hand, CO possesses some physiological roles as a signaling molecule that regulates neurotransmitters in addition to its hazardous effects. Because of the dual nature of CO, there is a need to develo...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10180483/ https://www.ncbi.nlm.nih.gov/pubmed/37175064 http://dx.doi.org/10.3390/molecules28093654 |
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author | Tange, Akari Kishikawa, Naoya Sakamoto, Yusuke El-Maghrabey, Mahmoud Wada, Mitsuhiro Kuroda, Naotaka |
author_facet | Tange, Akari Kishikawa, Naoya Sakamoto, Yusuke El-Maghrabey, Mahmoud Wada, Mitsuhiro Kuroda, Naotaka |
author_sort | Tange, Akari |
collection | PubMed |
description | Carbon monoxide (CO) is a toxic, hazardous gas that has a colorless and odorless nature. On the other hand, CO possesses some physiological roles as a signaling molecule that regulates neurotransmitters in addition to its hazardous effects. Because of the dual nature of CO, there is a need to develop a sensitive, selective, and rapid method for its detection. Herein, we designed and synthesized a turn-on fluorescence probe, 2-(2′-nitrophenyl)-4(3H)-quinazolinone (NPQ), for the detection of CO. NPQ provided a turn-on fluorescence response to CO and the fluorescence intensity at 500 nm was increased with increasing the concentration of CO. This fluorescence enhancement could be attributed to the conversion of the nitro group of NPQ to an amino group by the reducing ability of CO. The fluorescence assay for CO using NPQ as a reagent was confirmed to have a good linear relationship in the range of 1.0 to 50 µM with an excellent correlation coefficient (r) of 0.997 and good sensitivity down to a limit of detection at 0.73 µM (20 ppb) defined as mean blank+3SD. Finally, we successfully applied NPQ to the preparation of a test paper that can detect CO generated from charcoal combustion. |
format | Online Article Text |
id | pubmed-10180483 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101804832023-05-13 A Turn-On Quinazolinone-Based Fluorescence Probe for Selective Detection of Carbon Monoxide Tange, Akari Kishikawa, Naoya Sakamoto, Yusuke El-Maghrabey, Mahmoud Wada, Mitsuhiro Kuroda, Naotaka Molecules Article Carbon monoxide (CO) is a toxic, hazardous gas that has a colorless and odorless nature. On the other hand, CO possesses some physiological roles as a signaling molecule that regulates neurotransmitters in addition to its hazardous effects. Because of the dual nature of CO, there is a need to develop a sensitive, selective, and rapid method for its detection. Herein, we designed and synthesized a turn-on fluorescence probe, 2-(2′-nitrophenyl)-4(3H)-quinazolinone (NPQ), for the detection of CO. NPQ provided a turn-on fluorescence response to CO and the fluorescence intensity at 500 nm was increased with increasing the concentration of CO. This fluorescence enhancement could be attributed to the conversion of the nitro group of NPQ to an amino group by the reducing ability of CO. The fluorescence assay for CO using NPQ as a reagent was confirmed to have a good linear relationship in the range of 1.0 to 50 µM with an excellent correlation coefficient (r) of 0.997 and good sensitivity down to a limit of detection at 0.73 µM (20 ppb) defined as mean blank+3SD. Finally, we successfully applied NPQ to the preparation of a test paper that can detect CO generated from charcoal combustion. MDPI 2023-04-22 /pmc/articles/PMC10180483/ /pubmed/37175064 http://dx.doi.org/10.3390/molecules28093654 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tange, Akari Kishikawa, Naoya Sakamoto, Yusuke El-Maghrabey, Mahmoud Wada, Mitsuhiro Kuroda, Naotaka A Turn-On Quinazolinone-Based Fluorescence Probe for Selective Detection of Carbon Monoxide |
title | A Turn-On Quinazolinone-Based Fluorescence Probe for Selective Detection of Carbon Monoxide |
title_full | A Turn-On Quinazolinone-Based Fluorescence Probe for Selective Detection of Carbon Monoxide |
title_fullStr | A Turn-On Quinazolinone-Based Fluorescence Probe for Selective Detection of Carbon Monoxide |
title_full_unstemmed | A Turn-On Quinazolinone-Based Fluorescence Probe for Selective Detection of Carbon Monoxide |
title_short | A Turn-On Quinazolinone-Based Fluorescence Probe for Selective Detection of Carbon Monoxide |
title_sort | turn-on quinazolinone-based fluorescence probe for selective detection of carbon monoxide |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10180483/ https://www.ncbi.nlm.nih.gov/pubmed/37175064 http://dx.doi.org/10.3390/molecules28093654 |
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