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A Turn-On Quinazolinone-Based Fluorescence Probe for Selective Detection of Carbon Monoxide

Carbon monoxide (CO) is a toxic, hazardous gas that has a colorless and odorless nature. On the other hand, CO possesses some physiological roles as a signaling molecule that regulates neurotransmitters in addition to its hazardous effects. Because of the dual nature of CO, there is a need to develo...

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Autores principales: Tange, Akari, Kishikawa, Naoya, Sakamoto, Yusuke, El-Maghrabey, Mahmoud, Wada, Mitsuhiro, Kuroda, Naotaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10180483/
https://www.ncbi.nlm.nih.gov/pubmed/37175064
http://dx.doi.org/10.3390/molecules28093654
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author Tange, Akari
Kishikawa, Naoya
Sakamoto, Yusuke
El-Maghrabey, Mahmoud
Wada, Mitsuhiro
Kuroda, Naotaka
author_facet Tange, Akari
Kishikawa, Naoya
Sakamoto, Yusuke
El-Maghrabey, Mahmoud
Wada, Mitsuhiro
Kuroda, Naotaka
author_sort Tange, Akari
collection PubMed
description Carbon monoxide (CO) is a toxic, hazardous gas that has a colorless and odorless nature. On the other hand, CO possesses some physiological roles as a signaling molecule that regulates neurotransmitters in addition to its hazardous effects. Because of the dual nature of CO, there is a need to develop a sensitive, selective, and rapid method for its detection. Herein, we designed and synthesized a turn-on fluorescence probe, 2-(2′-nitrophenyl)-4(3H)-quinazolinone (NPQ), for the detection of CO. NPQ provided a turn-on fluorescence response to CO and the fluorescence intensity at 500 nm was increased with increasing the concentration of CO. This fluorescence enhancement could be attributed to the conversion of the nitro group of NPQ to an amino group by the reducing ability of CO. The fluorescence assay for CO using NPQ as a reagent was confirmed to have a good linear relationship in the range of 1.0 to 50 µM with an excellent correlation coefficient (r) of 0.997 and good sensitivity down to a limit of detection at 0.73 µM (20 ppb) defined as mean blank+3SD. Finally, we successfully applied NPQ to the preparation of a test paper that can detect CO generated from charcoal combustion.
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spelling pubmed-101804832023-05-13 A Turn-On Quinazolinone-Based Fluorescence Probe for Selective Detection of Carbon Monoxide Tange, Akari Kishikawa, Naoya Sakamoto, Yusuke El-Maghrabey, Mahmoud Wada, Mitsuhiro Kuroda, Naotaka Molecules Article Carbon monoxide (CO) is a toxic, hazardous gas that has a colorless and odorless nature. On the other hand, CO possesses some physiological roles as a signaling molecule that regulates neurotransmitters in addition to its hazardous effects. Because of the dual nature of CO, there is a need to develop a sensitive, selective, and rapid method for its detection. Herein, we designed and synthesized a turn-on fluorescence probe, 2-(2′-nitrophenyl)-4(3H)-quinazolinone (NPQ), for the detection of CO. NPQ provided a turn-on fluorescence response to CO and the fluorescence intensity at 500 nm was increased with increasing the concentration of CO. This fluorescence enhancement could be attributed to the conversion of the nitro group of NPQ to an amino group by the reducing ability of CO. The fluorescence assay for CO using NPQ as a reagent was confirmed to have a good linear relationship in the range of 1.0 to 50 µM with an excellent correlation coefficient (r) of 0.997 and good sensitivity down to a limit of detection at 0.73 µM (20 ppb) defined as mean blank+3SD. Finally, we successfully applied NPQ to the preparation of a test paper that can detect CO generated from charcoal combustion. MDPI 2023-04-22 /pmc/articles/PMC10180483/ /pubmed/37175064 http://dx.doi.org/10.3390/molecules28093654 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tange, Akari
Kishikawa, Naoya
Sakamoto, Yusuke
El-Maghrabey, Mahmoud
Wada, Mitsuhiro
Kuroda, Naotaka
A Turn-On Quinazolinone-Based Fluorescence Probe for Selective Detection of Carbon Monoxide
title A Turn-On Quinazolinone-Based Fluorescence Probe for Selective Detection of Carbon Monoxide
title_full A Turn-On Quinazolinone-Based Fluorescence Probe for Selective Detection of Carbon Monoxide
title_fullStr A Turn-On Quinazolinone-Based Fluorescence Probe for Selective Detection of Carbon Monoxide
title_full_unstemmed A Turn-On Quinazolinone-Based Fluorescence Probe for Selective Detection of Carbon Monoxide
title_short A Turn-On Quinazolinone-Based Fluorescence Probe for Selective Detection of Carbon Monoxide
title_sort turn-on quinazolinone-based fluorescence probe for selective detection of carbon monoxide
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10180483/
https://www.ncbi.nlm.nih.gov/pubmed/37175064
http://dx.doi.org/10.3390/molecules28093654
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