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Description of the Wild Strain Rhizobium rosettiformans DSM26376, Reclassified under Peteryoungia rosettiformans comb.nov., for Producing Glucuronan

Glucuronan is a polysaccharide composed of β-(1,4)-linked d-glucuronic acids having intrinsic properties and biological activities recoverable in many fields of application. Currently, the description of Sinorhyzobium meliloti M5N1CS mutant bacterial strain as the sole source of glucuronan makes it...

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Autores principales: Christophe, Gwendoline, Hou, Xiaoyang, Petit, Emmanuel, Traikia, Mounir, Le Cerf, Didier, Rihouey, Christophe, Gardarin, Christine, Delattre, Cédric, Michaud, Philippe, Pierre, Guillaume, Dubessay, Pascal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10180729/
https://www.ncbi.nlm.nih.gov/pubmed/37177323
http://dx.doi.org/10.3390/polym15092177
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author Christophe, Gwendoline
Hou, Xiaoyang
Petit, Emmanuel
Traikia, Mounir
Le Cerf, Didier
Rihouey, Christophe
Gardarin, Christine
Delattre, Cédric
Michaud, Philippe
Pierre, Guillaume
Dubessay, Pascal
author_facet Christophe, Gwendoline
Hou, Xiaoyang
Petit, Emmanuel
Traikia, Mounir
Le Cerf, Didier
Rihouey, Christophe
Gardarin, Christine
Delattre, Cédric
Michaud, Philippe
Pierre, Guillaume
Dubessay, Pascal
author_sort Christophe, Gwendoline
collection PubMed
description Glucuronan is a polysaccharide composed of β-(1,4)-linked d-glucuronic acids having intrinsic properties and biological activities recoverable in many fields of application. Currently, the description of Sinorhyzobium meliloti M5N1CS mutant bacterial strain as the sole source of glucuronan makes it relevant to the exploration of new microorganisms producing glucuronan. In this study, the Peteryoungia rosettifformans strain (Rhizobia), was identified as a wild producer of an exopolysaccharide (RhrBR46) related to glucuronan. Structural and biochemical features, using colorimetric assays, Fourier infrared spectroscopy, nuclear magnetic resonance, high pressure size exclusion chromatography coupled to multi-angle light laser scattering, and enzymatic assays allowed the characterization of a polyglucuronic acid, having a molecular mass ([Formula: see text]) of 1.85 × 10(5) Da, and being partially O-acetylated at C-2 and/or C-3 positions. The concentration of Mg(2+) ions in the cultivation medium has been shown to impact the structure of RhrBR46, by reducing drastically its [Formula: see text] (73%) and increasing its DA (10%). Comparative structural analyses between RhrBR46 and the glucuronan from Sinorhyzobium meliloti M5N1CS strain revealed differences in terms of molecular weight, degree of acetylation (DA), and the distribution of acetylation pattern. These structural divergences of RhrBR46 might contribute to singular properties or biological activities of RhrBR46, offering new perspectives of application.
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spelling pubmed-101807292023-05-13 Description of the Wild Strain Rhizobium rosettiformans DSM26376, Reclassified under Peteryoungia rosettiformans comb.nov., for Producing Glucuronan Christophe, Gwendoline Hou, Xiaoyang Petit, Emmanuel Traikia, Mounir Le Cerf, Didier Rihouey, Christophe Gardarin, Christine Delattre, Cédric Michaud, Philippe Pierre, Guillaume Dubessay, Pascal Polymers (Basel) Article Glucuronan is a polysaccharide composed of β-(1,4)-linked d-glucuronic acids having intrinsic properties and biological activities recoverable in many fields of application. Currently, the description of Sinorhyzobium meliloti M5N1CS mutant bacterial strain as the sole source of glucuronan makes it relevant to the exploration of new microorganisms producing glucuronan. In this study, the Peteryoungia rosettifformans strain (Rhizobia), was identified as a wild producer of an exopolysaccharide (RhrBR46) related to glucuronan. Structural and biochemical features, using colorimetric assays, Fourier infrared spectroscopy, nuclear magnetic resonance, high pressure size exclusion chromatography coupled to multi-angle light laser scattering, and enzymatic assays allowed the characterization of a polyglucuronic acid, having a molecular mass ([Formula: see text]) of 1.85 × 10(5) Da, and being partially O-acetylated at C-2 and/or C-3 positions. The concentration of Mg(2+) ions in the cultivation medium has been shown to impact the structure of RhrBR46, by reducing drastically its [Formula: see text] (73%) and increasing its DA (10%). Comparative structural analyses between RhrBR46 and the glucuronan from Sinorhyzobium meliloti M5N1CS strain revealed differences in terms of molecular weight, degree of acetylation (DA), and the distribution of acetylation pattern. These structural divergences of RhrBR46 might contribute to singular properties or biological activities of RhrBR46, offering new perspectives of application. MDPI 2023-05-03 /pmc/articles/PMC10180729/ /pubmed/37177323 http://dx.doi.org/10.3390/polym15092177 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Christophe, Gwendoline
Hou, Xiaoyang
Petit, Emmanuel
Traikia, Mounir
Le Cerf, Didier
Rihouey, Christophe
Gardarin, Christine
Delattre, Cédric
Michaud, Philippe
Pierre, Guillaume
Dubessay, Pascal
Description of the Wild Strain Rhizobium rosettiformans DSM26376, Reclassified under Peteryoungia rosettiformans comb.nov., for Producing Glucuronan
title Description of the Wild Strain Rhizobium rosettiformans DSM26376, Reclassified under Peteryoungia rosettiformans comb.nov., for Producing Glucuronan
title_full Description of the Wild Strain Rhizobium rosettiformans DSM26376, Reclassified under Peteryoungia rosettiformans comb.nov., for Producing Glucuronan
title_fullStr Description of the Wild Strain Rhizobium rosettiformans DSM26376, Reclassified under Peteryoungia rosettiformans comb.nov., for Producing Glucuronan
title_full_unstemmed Description of the Wild Strain Rhizobium rosettiformans DSM26376, Reclassified under Peteryoungia rosettiformans comb.nov., for Producing Glucuronan
title_short Description of the Wild Strain Rhizobium rosettiformans DSM26376, Reclassified under Peteryoungia rosettiformans comb.nov., for Producing Glucuronan
title_sort description of the wild strain rhizobium rosettiformans dsm26376, reclassified under peteryoungia rosettiformans comb.nov., for producing glucuronan
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10180729/
https://www.ncbi.nlm.nih.gov/pubmed/37177323
http://dx.doi.org/10.3390/polym15092177
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