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Cytoplasmic Kinase Network Mediates Defense Response to Spodoptera litura in Arabidopsis

Plants defend against folivores by responding to folivore-derived elicitors following activation of signaling cascade networks. In Arabidopsis, HAK1, a receptor-like kinase, responds to polysaccharide elicitors (Frα) that are present in oral secretions of Spodoptera litura larvae to upregulate defen...

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Autores principales: Desaki, Yoshitake, Morishima, Minami, Sano, Yuka, Uemura, Takuya, Ito, Ayaka, Nemoto, Keiichirou, Nozawa, Akira, Sawasaki, Tatsuya, Arimura, Gen-ichiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10180863/
https://www.ncbi.nlm.nih.gov/pubmed/37176805
http://dx.doi.org/10.3390/plants12091747
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author Desaki, Yoshitake
Morishima, Minami
Sano, Yuka
Uemura, Takuya
Ito, Ayaka
Nemoto, Keiichirou
Nozawa, Akira
Sawasaki, Tatsuya
Arimura, Gen-ichiro
author_facet Desaki, Yoshitake
Morishima, Minami
Sano, Yuka
Uemura, Takuya
Ito, Ayaka
Nemoto, Keiichirou
Nozawa, Akira
Sawasaki, Tatsuya
Arimura, Gen-ichiro
author_sort Desaki, Yoshitake
collection PubMed
description Plants defend against folivores by responding to folivore-derived elicitors following activation of signaling cascade networks. In Arabidopsis, HAK1, a receptor-like kinase, responds to polysaccharide elicitors (Frα) that are present in oral secretions of Spodoptera litura larvae to upregulate defense genes (e.g., PDF1.2) mediated through downstream cytoplasmic kinase PBL27. Here, we explored whether other protein kinases, including CPKs and CRKs, function with PBL27 in the intracellular signaling network for anti-herbivore responses. We showed that CRK2 and CRK3 were found to interact with PBL27, but CPKs did not. Although transcripts of PDF1.2 were upregulated in leaves of wild-type Arabidopsis plants in response to mechanical damage with Frα, this failed in CRK2- and PBL27-deficient mutant plants, indicating that the CRK2/PBL27 system is predominantly responsible for the Frα-responsive transcription of PDF1.2 in S. litura-damaged plants. In addition to CRK2-phosphorylated ERF13, as shown previously, ethylene signaling in connection to CRK2-phosphorylated PBL27 was predicted to be responsible for transcriptional regulation of a gene for ethylene response factor 13 (ERF13). Taken together, these findings show that CRK2 regulates not only ERF13 phosphorylation but also PBL27-dependent de novo synthesis of ERF13, thus determining active defense traits against S. litura larvae via transcriptional regulation of PDF1.2.
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spelling pubmed-101808632023-05-13 Cytoplasmic Kinase Network Mediates Defense Response to Spodoptera litura in Arabidopsis Desaki, Yoshitake Morishima, Minami Sano, Yuka Uemura, Takuya Ito, Ayaka Nemoto, Keiichirou Nozawa, Akira Sawasaki, Tatsuya Arimura, Gen-ichiro Plants (Basel) Article Plants defend against folivores by responding to folivore-derived elicitors following activation of signaling cascade networks. In Arabidopsis, HAK1, a receptor-like kinase, responds to polysaccharide elicitors (Frα) that are present in oral secretions of Spodoptera litura larvae to upregulate defense genes (e.g., PDF1.2) mediated through downstream cytoplasmic kinase PBL27. Here, we explored whether other protein kinases, including CPKs and CRKs, function with PBL27 in the intracellular signaling network for anti-herbivore responses. We showed that CRK2 and CRK3 were found to interact with PBL27, but CPKs did not. Although transcripts of PDF1.2 were upregulated in leaves of wild-type Arabidopsis plants in response to mechanical damage with Frα, this failed in CRK2- and PBL27-deficient mutant plants, indicating that the CRK2/PBL27 system is predominantly responsible for the Frα-responsive transcription of PDF1.2 in S. litura-damaged plants. In addition to CRK2-phosphorylated ERF13, as shown previously, ethylene signaling in connection to CRK2-phosphorylated PBL27 was predicted to be responsible for transcriptional regulation of a gene for ethylene response factor 13 (ERF13). Taken together, these findings show that CRK2 regulates not only ERF13 phosphorylation but also PBL27-dependent de novo synthesis of ERF13, thus determining active defense traits against S. litura larvae via transcriptional regulation of PDF1.2. MDPI 2023-04-24 /pmc/articles/PMC10180863/ /pubmed/37176805 http://dx.doi.org/10.3390/plants12091747 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Desaki, Yoshitake
Morishima, Minami
Sano, Yuka
Uemura, Takuya
Ito, Ayaka
Nemoto, Keiichirou
Nozawa, Akira
Sawasaki, Tatsuya
Arimura, Gen-ichiro
Cytoplasmic Kinase Network Mediates Defense Response to Spodoptera litura in Arabidopsis
title Cytoplasmic Kinase Network Mediates Defense Response to Spodoptera litura in Arabidopsis
title_full Cytoplasmic Kinase Network Mediates Defense Response to Spodoptera litura in Arabidopsis
title_fullStr Cytoplasmic Kinase Network Mediates Defense Response to Spodoptera litura in Arabidopsis
title_full_unstemmed Cytoplasmic Kinase Network Mediates Defense Response to Spodoptera litura in Arabidopsis
title_short Cytoplasmic Kinase Network Mediates Defense Response to Spodoptera litura in Arabidopsis
title_sort cytoplasmic kinase network mediates defense response to spodoptera litura in arabidopsis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10180863/
https://www.ncbi.nlm.nih.gov/pubmed/37176805
http://dx.doi.org/10.3390/plants12091747
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