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In Depth Topological Analysis of Arabidopsis Mid-SUN Proteins and Their Interaction with the Membrane-Bound Transcription Factor MaMYB
Mid-SUN proteins are a neglected family of conserved type III membrane proteins of ancient origin with representatives in plants, animals, and fungi. Previous higher plant studies have associated them with functions at the nuclear envelope and the endoplasmic reticulum (ER). In this study, high-reso...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10180911/ https://www.ncbi.nlm.nih.gov/pubmed/37176845 http://dx.doi.org/10.3390/plants12091787 |
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author | Andov, Bisa Boulaflous-Stevens, Aurelia Pain, Charlotte Mermet, Sarah Voisin, Maxime Charrondiere, Camille Vanrobays, Emmanuel Tutois, Sylvie Evans, David E. Kriechbaumer, Verena Tatout, Christophe Graumann, Katja |
author_facet | Andov, Bisa Boulaflous-Stevens, Aurelia Pain, Charlotte Mermet, Sarah Voisin, Maxime Charrondiere, Camille Vanrobays, Emmanuel Tutois, Sylvie Evans, David E. Kriechbaumer, Verena Tatout, Christophe Graumann, Katja |
author_sort | Andov, Bisa |
collection | PubMed |
description | Mid-SUN proteins are a neglected family of conserved type III membrane proteins of ancient origin with representatives in plants, animals, and fungi. Previous higher plant studies have associated them with functions at the nuclear envelope and the endoplasmic reticulum (ER). In this study, high-resolution confocal light microscopy is used to explore the localisation of SUN3 and SUN4 in the perinuclear region, to explore topology, and to study the role of mid-SUNs on endoplasmic reticulum morphology. The role of SUN3 in the ER is reinforced by the identification of a protein interaction between SUN3 and the ER membrane-bound transcription factor maMYB. The results highlight the importance of mid-SUNs as functional components of the ER and outer nuclear membrane. |
format | Online Article Text |
id | pubmed-10180911 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101809112023-05-13 In Depth Topological Analysis of Arabidopsis Mid-SUN Proteins and Their Interaction with the Membrane-Bound Transcription Factor MaMYB Andov, Bisa Boulaflous-Stevens, Aurelia Pain, Charlotte Mermet, Sarah Voisin, Maxime Charrondiere, Camille Vanrobays, Emmanuel Tutois, Sylvie Evans, David E. Kriechbaumer, Verena Tatout, Christophe Graumann, Katja Plants (Basel) Article Mid-SUN proteins are a neglected family of conserved type III membrane proteins of ancient origin with representatives in plants, animals, and fungi. Previous higher plant studies have associated them with functions at the nuclear envelope and the endoplasmic reticulum (ER). In this study, high-resolution confocal light microscopy is used to explore the localisation of SUN3 and SUN4 in the perinuclear region, to explore topology, and to study the role of mid-SUNs on endoplasmic reticulum morphology. The role of SUN3 in the ER is reinforced by the identification of a protein interaction between SUN3 and the ER membrane-bound transcription factor maMYB. The results highlight the importance of mid-SUNs as functional components of the ER and outer nuclear membrane. MDPI 2023-04-27 /pmc/articles/PMC10180911/ /pubmed/37176845 http://dx.doi.org/10.3390/plants12091787 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Andov, Bisa Boulaflous-Stevens, Aurelia Pain, Charlotte Mermet, Sarah Voisin, Maxime Charrondiere, Camille Vanrobays, Emmanuel Tutois, Sylvie Evans, David E. Kriechbaumer, Verena Tatout, Christophe Graumann, Katja In Depth Topological Analysis of Arabidopsis Mid-SUN Proteins and Their Interaction with the Membrane-Bound Transcription Factor MaMYB |
title | In Depth Topological Analysis of Arabidopsis Mid-SUN Proteins and Their Interaction with the Membrane-Bound Transcription Factor MaMYB |
title_full | In Depth Topological Analysis of Arabidopsis Mid-SUN Proteins and Their Interaction with the Membrane-Bound Transcription Factor MaMYB |
title_fullStr | In Depth Topological Analysis of Arabidopsis Mid-SUN Proteins and Their Interaction with the Membrane-Bound Transcription Factor MaMYB |
title_full_unstemmed | In Depth Topological Analysis of Arabidopsis Mid-SUN Proteins and Their Interaction with the Membrane-Bound Transcription Factor MaMYB |
title_short | In Depth Topological Analysis of Arabidopsis Mid-SUN Proteins and Their Interaction with the Membrane-Bound Transcription Factor MaMYB |
title_sort | in depth topological analysis of arabidopsis mid-sun proteins and their interaction with the membrane-bound transcription factor mamyb |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10180911/ https://www.ncbi.nlm.nih.gov/pubmed/37176845 http://dx.doi.org/10.3390/plants12091787 |
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