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In Depth Topological Analysis of Arabidopsis Mid-SUN Proteins and Their Interaction with the Membrane-Bound Transcription Factor MaMYB

Mid-SUN proteins are a neglected family of conserved type III membrane proteins of ancient origin with representatives in plants, animals, and fungi. Previous higher plant studies have associated them with functions at the nuclear envelope and the endoplasmic reticulum (ER). In this study, high-reso...

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Autores principales: Andov, Bisa, Boulaflous-Stevens, Aurelia, Pain, Charlotte, Mermet, Sarah, Voisin, Maxime, Charrondiere, Camille, Vanrobays, Emmanuel, Tutois, Sylvie, Evans, David E., Kriechbaumer, Verena, Tatout, Christophe, Graumann, Katja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10180911/
https://www.ncbi.nlm.nih.gov/pubmed/37176845
http://dx.doi.org/10.3390/plants12091787
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author Andov, Bisa
Boulaflous-Stevens, Aurelia
Pain, Charlotte
Mermet, Sarah
Voisin, Maxime
Charrondiere, Camille
Vanrobays, Emmanuel
Tutois, Sylvie
Evans, David E.
Kriechbaumer, Verena
Tatout, Christophe
Graumann, Katja
author_facet Andov, Bisa
Boulaflous-Stevens, Aurelia
Pain, Charlotte
Mermet, Sarah
Voisin, Maxime
Charrondiere, Camille
Vanrobays, Emmanuel
Tutois, Sylvie
Evans, David E.
Kriechbaumer, Verena
Tatout, Christophe
Graumann, Katja
author_sort Andov, Bisa
collection PubMed
description Mid-SUN proteins are a neglected family of conserved type III membrane proteins of ancient origin with representatives in plants, animals, and fungi. Previous higher plant studies have associated them with functions at the nuclear envelope and the endoplasmic reticulum (ER). In this study, high-resolution confocal light microscopy is used to explore the localisation of SUN3 and SUN4 in the perinuclear region, to explore topology, and to study the role of mid-SUNs on endoplasmic reticulum morphology. The role of SUN3 in the ER is reinforced by the identification of a protein interaction between SUN3 and the ER membrane-bound transcription factor maMYB. The results highlight the importance of mid-SUNs as functional components of the ER and outer nuclear membrane.
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spelling pubmed-101809112023-05-13 In Depth Topological Analysis of Arabidopsis Mid-SUN Proteins and Their Interaction with the Membrane-Bound Transcription Factor MaMYB Andov, Bisa Boulaflous-Stevens, Aurelia Pain, Charlotte Mermet, Sarah Voisin, Maxime Charrondiere, Camille Vanrobays, Emmanuel Tutois, Sylvie Evans, David E. Kriechbaumer, Verena Tatout, Christophe Graumann, Katja Plants (Basel) Article Mid-SUN proteins are a neglected family of conserved type III membrane proteins of ancient origin with representatives in plants, animals, and fungi. Previous higher plant studies have associated them with functions at the nuclear envelope and the endoplasmic reticulum (ER). In this study, high-resolution confocal light microscopy is used to explore the localisation of SUN3 and SUN4 in the perinuclear region, to explore topology, and to study the role of mid-SUNs on endoplasmic reticulum morphology. The role of SUN3 in the ER is reinforced by the identification of a protein interaction between SUN3 and the ER membrane-bound transcription factor maMYB. The results highlight the importance of mid-SUNs as functional components of the ER and outer nuclear membrane. MDPI 2023-04-27 /pmc/articles/PMC10180911/ /pubmed/37176845 http://dx.doi.org/10.3390/plants12091787 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Andov, Bisa
Boulaflous-Stevens, Aurelia
Pain, Charlotte
Mermet, Sarah
Voisin, Maxime
Charrondiere, Camille
Vanrobays, Emmanuel
Tutois, Sylvie
Evans, David E.
Kriechbaumer, Verena
Tatout, Christophe
Graumann, Katja
In Depth Topological Analysis of Arabidopsis Mid-SUN Proteins and Their Interaction with the Membrane-Bound Transcription Factor MaMYB
title In Depth Topological Analysis of Arabidopsis Mid-SUN Proteins and Their Interaction with the Membrane-Bound Transcription Factor MaMYB
title_full In Depth Topological Analysis of Arabidopsis Mid-SUN Proteins and Their Interaction with the Membrane-Bound Transcription Factor MaMYB
title_fullStr In Depth Topological Analysis of Arabidopsis Mid-SUN Proteins and Their Interaction with the Membrane-Bound Transcription Factor MaMYB
title_full_unstemmed In Depth Topological Analysis of Arabidopsis Mid-SUN Proteins and Their Interaction with the Membrane-Bound Transcription Factor MaMYB
title_short In Depth Topological Analysis of Arabidopsis Mid-SUN Proteins and Their Interaction with the Membrane-Bound Transcription Factor MaMYB
title_sort in depth topological analysis of arabidopsis mid-sun proteins and their interaction with the membrane-bound transcription factor mamyb
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10180911/
https://www.ncbi.nlm.nih.gov/pubmed/37176845
http://dx.doi.org/10.3390/plants12091787
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