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Effect of Low Temperature on Insecticidal Protein Contents of Cotton (Gossypium herbaceum L.) in the Boll Shell and Its Physiological Mechanism

Low temperature is the main factor for global natural disasters affecting the growth and distribution of plants, and cotton may be affected by low temperature and cold damage at all growth stages. In addition, the insecticidal resistance of cultivars has been reported to perform poorly or unstably d...

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Autores principales: Liu, Zhenyu, Ji, Mingyu, He, Run, Dai, Yuyang, Liu, Yuting, Mou, Nana, Du, Jianing, Zhang, Xiang, Chen, Dehua, Chen, Yuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10180954/
https://www.ncbi.nlm.nih.gov/pubmed/37176825
http://dx.doi.org/10.3390/plants12091767
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author Liu, Zhenyu
Ji, Mingyu
He, Run
Dai, Yuyang
Liu, Yuting
Mou, Nana
Du, Jianing
Zhang, Xiang
Chen, Dehua
Chen, Yuan
author_facet Liu, Zhenyu
Ji, Mingyu
He, Run
Dai, Yuyang
Liu, Yuting
Mou, Nana
Du, Jianing
Zhang, Xiang
Chen, Dehua
Chen, Yuan
author_sort Liu, Zhenyu
collection PubMed
description Low temperature is the main factor for global natural disasters affecting the growth and distribution of plants, and cotton may be affected by low temperature and cold damage at all growth stages. In addition, the insecticidal resistance of cultivars has been reported to perform poorly or unstably due to adverse environments. The present study aimed to investigate the impact of low temperature on the levels of insecticidal protein in Bacillus thuringiensis (Bt) transgenic cotton plants during the peak boll stage. To achieve this, two Bt cotton cultivars, Sikang1 (SK1) and Sikang3 (SK3), were subjected to different temperature regimes and durations. The findings of the study demonstrated that the expression of insecticidal protein in the boll shell of Bt transgenic cotton plants was significantly inhibited under low-temperature stress. Specifically, in 2020, compared to the CK (27 °C), the insecticidal protein content in the boll shell of SK3 decreased by 28.19% after a 48 h of a 16 °C temperature. These results suggest that low-temperature stress can negatively impact the expression of insecticidal protein in Bt transgenic cotton, highlighting the need for appropriate measures to minimize its adverse effects on cotton production. In addition, the threshold temperature that leads to a significant decrease in the content of insecticidal proteins symbolizes an upward trend as the duration of stress prolongs. Decreased Bt protein content at low temperatures is associated with changes in the N metabolism. The present study revealed a significant positive correlation between the levels of glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT) activities, as well as in the soluble protein levels in the boll shell and the content of the Bt protein. On the other hand, a significant negative correlation was observed between the levels of free amino acids, peptidase, and protease activities, as well as of Bt protein content. These findings suggest that, in Bt cotton production, it is crucial to remain vigilant of prolonged low-temperature disasters, which last for over 12 h and drop below 17–20 °C during the peak boll stage. Such conditions may reduce insecticidal resistance, leading to substantial economic losses.
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spelling pubmed-101809542023-05-13 Effect of Low Temperature on Insecticidal Protein Contents of Cotton (Gossypium herbaceum L.) in the Boll Shell and Its Physiological Mechanism Liu, Zhenyu Ji, Mingyu He, Run Dai, Yuyang Liu, Yuting Mou, Nana Du, Jianing Zhang, Xiang Chen, Dehua Chen, Yuan Plants (Basel) Article Low temperature is the main factor for global natural disasters affecting the growth and distribution of plants, and cotton may be affected by low temperature and cold damage at all growth stages. In addition, the insecticidal resistance of cultivars has been reported to perform poorly or unstably due to adverse environments. The present study aimed to investigate the impact of low temperature on the levels of insecticidal protein in Bacillus thuringiensis (Bt) transgenic cotton plants during the peak boll stage. To achieve this, two Bt cotton cultivars, Sikang1 (SK1) and Sikang3 (SK3), were subjected to different temperature regimes and durations. The findings of the study demonstrated that the expression of insecticidal protein in the boll shell of Bt transgenic cotton plants was significantly inhibited under low-temperature stress. Specifically, in 2020, compared to the CK (27 °C), the insecticidal protein content in the boll shell of SK3 decreased by 28.19% after a 48 h of a 16 °C temperature. These results suggest that low-temperature stress can negatively impact the expression of insecticidal protein in Bt transgenic cotton, highlighting the need for appropriate measures to minimize its adverse effects on cotton production. In addition, the threshold temperature that leads to a significant decrease in the content of insecticidal proteins symbolizes an upward trend as the duration of stress prolongs. Decreased Bt protein content at low temperatures is associated with changes in the N metabolism. The present study revealed a significant positive correlation between the levels of glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT) activities, as well as in the soluble protein levels in the boll shell and the content of the Bt protein. On the other hand, a significant negative correlation was observed between the levels of free amino acids, peptidase, and protease activities, as well as of Bt protein content. These findings suggest that, in Bt cotton production, it is crucial to remain vigilant of prolonged low-temperature disasters, which last for over 12 h and drop below 17–20 °C during the peak boll stage. Such conditions may reduce insecticidal resistance, leading to substantial economic losses. MDPI 2023-04-26 /pmc/articles/PMC10180954/ /pubmed/37176825 http://dx.doi.org/10.3390/plants12091767 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Zhenyu
Ji, Mingyu
He, Run
Dai, Yuyang
Liu, Yuting
Mou, Nana
Du, Jianing
Zhang, Xiang
Chen, Dehua
Chen, Yuan
Effect of Low Temperature on Insecticidal Protein Contents of Cotton (Gossypium herbaceum L.) in the Boll Shell and Its Physiological Mechanism
title Effect of Low Temperature on Insecticidal Protein Contents of Cotton (Gossypium herbaceum L.) in the Boll Shell and Its Physiological Mechanism
title_full Effect of Low Temperature on Insecticidal Protein Contents of Cotton (Gossypium herbaceum L.) in the Boll Shell and Its Physiological Mechanism
title_fullStr Effect of Low Temperature on Insecticidal Protein Contents of Cotton (Gossypium herbaceum L.) in the Boll Shell and Its Physiological Mechanism
title_full_unstemmed Effect of Low Temperature on Insecticidal Protein Contents of Cotton (Gossypium herbaceum L.) in the Boll Shell and Its Physiological Mechanism
title_short Effect of Low Temperature on Insecticidal Protein Contents of Cotton (Gossypium herbaceum L.) in the Boll Shell and Its Physiological Mechanism
title_sort effect of low temperature on insecticidal protein contents of cotton (gossypium herbaceum l.) in the boll shell and its physiological mechanism
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10180954/
https://www.ncbi.nlm.nih.gov/pubmed/37176825
http://dx.doi.org/10.3390/plants12091767
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