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Broadband Electrical Spectroscopy to Distinguish Single-Cell Ca(2+) Changes Due to Ionomycin Treatment in a Skeletal Muscle Cell Line
Many skeletal muscle diseases such as muscular dystrophy, myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS), and sarcopenia share the dysregulation of calcium (Ca(2+)) as a key mechanism of disease at a cellular level. Cytosolic concentrations of Ca(2+) can signal dysregulation in organell...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10181519/ https://www.ncbi.nlm.nih.gov/pubmed/37177559 http://dx.doi.org/10.3390/s23094358 |
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author | Ferguson, Caroline A. Santangelo, Carmen Marramiero, Lorenzo Farina, Marco Pietrangelo, Tiziana Cheng, Xuanhong |
author_facet | Ferguson, Caroline A. Santangelo, Carmen Marramiero, Lorenzo Farina, Marco Pietrangelo, Tiziana Cheng, Xuanhong |
author_sort | Ferguson, Caroline A. |
collection | PubMed |
description | Many skeletal muscle diseases such as muscular dystrophy, myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS), and sarcopenia share the dysregulation of calcium (Ca(2+)) as a key mechanism of disease at a cellular level. Cytosolic concentrations of Ca(2+) can signal dysregulation in organelles including the mitochondria, nucleus, and sarcoplasmic reticulum in skeletal muscle. In this work, a treatment is applied to mimic the Ca(2+) increase associated with these atrophy-related disease states, and broadband impedance measurements are taken for single cells with and without this treatment using a microfluidic device. The resulting impedance measurements are fitted using a single-shell circuit simulation to show calculated electrical dielectric property contributions based on these Ca(2+) changes. From this, similar distributions were seen in the Ca(2+) from fluorescence measurements and the distribution of the S-parameter at a single frequency, identifying Ca(2+) as the main contributor to the electrical differences being identified. Extracted dielectric parameters also showed different distribution patterns between the untreated and ionomycin-treated groups; however, the overall electrical parameters suggest the impact of Ca(2+)-induced changes at a wider range of frequencies. |
format | Online Article Text |
id | pubmed-10181519 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101815192023-05-13 Broadband Electrical Spectroscopy to Distinguish Single-Cell Ca(2+) Changes Due to Ionomycin Treatment in a Skeletal Muscle Cell Line Ferguson, Caroline A. Santangelo, Carmen Marramiero, Lorenzo Farina, Marco Pietrangelo, Tiziana Cheng, Xuanhong Sensors (Basel) Article Many skeletal muscle diseases such as muscular dystrophy, myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS), and sarcopenia share the dysregulation of calcium (Ca(2+)) as a key mechanism of disease at a cellular level. Cytosolic concentrations of Ca(2+) can signal dysregulation in organelles including the mitochondria, nucleus, and sarcoplasmic reticulum in skeletal muscle. In this work, a treatment is applied to mimic the Ca(2+) increase associated with these atrophy-related disease states, and broadband impedance measurements are taken for single cells with and without this treatment using a microfluidic device. The resulting impedance measurements are fitted using a single-shell circuit simulation to show calculated electrical dielectric property contributions based on these Ca(2+) changes. From this, similar distributions were seen in the Ca(2+) from fluorescence measurements and the distribution of the S-parameter at a single frequency, identifying Ca(2+) as the main contributor to the electrical differences being identified. Extracted dielectric parameters also showed different distribution patterns between the untreated and ionomycin-treated groups; however, the overall electrical parameters suggest the impact of Ca(2+)-induced changes at a wider range of frequencies. MDPI 2023-04-28 /pmc/articles/PMC10181519/ /pubmed/37177559 http://dx.doi.org/10.3390/s23094358 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ferguson, Caroline A. Santangelo, Carmen Marramiero, Lorenzo Farina, Marco Pietrangelo, Tiziana Cheng, Xuanhong Broadband Electrical Spectroscopy to Distinguish Single-Cell Ca(2+) Changes Due to Ionomycin Treatment in a Skeletal Muscle Cell Line |
title | Broadband Electrical Spectroscopy to Distinguish Single-Cell Ca(2+) Changes Due to Ionomycin Treatment in a Skeletal Muscle Cell Line |
title_full | Broadband Electrical Spectroscopy to Distinguish Single-Cell Ca(2+) Changes Due to Ionomycin Treatment in a Skeletal Muscle Cell Line |
title_fullStr | Broadband Electrical Spectroscopy to Distinguish Single-Cell Ca(2+) Changes Due to Ionomycin Treatment in a Skeletal Muscle Cell Line |
title_full_unstemmed | Broadband Electrical Spectroscopy to Distinguish Single-Cell Ca(2+) Changes Due to Ionomycin Treatment in a Skeletal Muscle Cell Line |
title_short | Broadband Electrical Spectroscopy to Distinguish Single-Cell Ca(2+) Changes Due to Ionomycin Treatment in a Skeletal Muscle Cell Line |
title_sort | broadband electrical spectroscopy to distinguish single-cell ca(2+) changes due to ionomycin treatment in a skeletal muscle cell line |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10181519/ https://www.ncbi.nlm.nih.gov/pubmed/37177559 http://dx.doi.org/10.3390/s23094358 |
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