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Broadband Electrical Spectroscopy to Distinguish Single-Cell Ca(2+) Changes Due to Ionomycin Treatment in a Skeletal Muscle Cell Line

Many skeletal muscle diseases such as muscular dystrophy, myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS), and sarcopenia share the dysregulation of calcium (Ca(2+)) as a key mechanism of disease at a cellular level. Cytosolic concentrations of Ca(2+) can signal dysregulation in organell...

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Autores principales: Ferguson, Caroline A., Santangelo, Carmen, Marramiero, Lorenzo, Farina, Marco, Pietrangelo, Tiziana, Cheng, Xuanhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10181519/
https://www.ncbi.nlm.nih.gov/pubmed/37177559
http://dx.doi.org/10.3390/s23094358
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author Ferguson, Caroline A.
Santangelo, Carmen
Marramiero, Lorenzo
Farina, Marco
Pietrangelo, Tiziana
Cheng, Xuanhong
author_facet Ferguson, Caroline A.
Santangelo, Carmen
Marramiero, Lorenzo
Farina, Marco
Pietrangelo, Tiziana
Cheng, Xuanhong
author_sort Ferguson, Caroline A.
collection PubMed
description Many skeletal muscle diseases such as muscular dystrophy, myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS), and sarcopenia share the dysregulation of calcium (Ca(2+)) as a key mechanism of disease at a cellular level. Cytosolic concentrations of Ca(2+) can signal dysregulation in organelles including the mitochondria, nucleus, and sarcoplasmic reticulum in skeletal muscle. In this work, a treatment is applied to mimic the Ca(2+) increase associated with these atrophy-related disease states, and broadband impedance measurements are taken for single cells with and without this treatment using a microfluidic device. The resulting impedance measurements are fitted using a single-shell circuit simulation to show calculated electrical dielectric property contributions based on these Ca(2+) changes. From this, similar distributions were seen in the Ca(2+) from fluorescence measurements and the distribution of the S-parameter at a single frequency, identifying Ca(2+) as the main contributor to the electrical differences being identified. Extracted dielectric parameters also showed different distribution patterns between the untreated and ionomycin-treated groups; however, the overall electrical parameters suggest the impact of Ca(2+)-induced changes at a wider range of frequencies.
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spelling pubmed-101815192023-05-13 Broadband Electrical Spectroscopy to Distinguish Single-Cell Ca(2+) Changes Due to Ionomycin Treatment in a Skeletal Muscle Cell Line Ferguson, Caroline A. Santangelo, Carmen Marramiero, Lorenzo Farina, Marco Pietrangelo, Tiziana Cheng, Xuanhong Sensors (Basel) Article Many skeletal muscle diseases such as muscular dystrophy, myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS), and sarcopenia share the dysregulation of calcium (Ca(2+)) as a key mechanism of disease at a cellular level. Cytosolic concentrations of Ca(2+) can signal dysregulation in organelles including the mitochondria, nucleus, and sarcoplasmic reticulum in skeletal muscle. In this work, a treatment is applied to mimic the Ca(2+) increase associated with these atrophy-related disease states, and broadband impedance measurements are taken for single cells with and without this treatment using a microfluidic device. The resulting impedance measurements are fitted using a single-shell circuit simulation to show calculated electrical dielectric property contributions based on these Ca(2+) changes. From this, similar distributions were seen in the Ca(2+) from fluorescence measurements and the distribution of the S-parameter at a single frequency, identifying Ca(2+) as the main contributor to the electrical differences being identified. Extracted dielectric parameters also showed different distribution patterns between the untreated and ionomycin-treated groups; however, the overall electrical parameters suggest the impact of Ca(2+)-induced changes at a wider range of frequencies. MDPI 2023-04-28 /pmc/articles/PMC10181519/ /pubmed/37177559 http://dx.doi.org/10.3390/s23094358 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ferguson, Caroline A.
Santangelo, Carmen
Marramiero, Lorenzo
Farina, Marco
Pietrangelo, Tiziana
Cheng, Xuanhong
Broadband Electrical Spectroscopy to Distinguish Single-Cell Ca(2+) Changes Due to Ionomycin Treatment in a Skeletal Muscle Cell Line
title Broadband Electrical Spectroscopy to Distinguish Single-Cell Ca(2+) Changes Due to Ionomycin Treatment in a Skeletal Muscle Cell Line
title_full Broadband Electrical Spectroscopy to Distinguish Single-Cell Ca(2+) Changes Due to Ionomycin Treatment in a Skeletal Muscle Cell Line
title_fullStr Broadband Electrical Spectroscopy to Distinguish Single-Cell Ca(2+) Changes Due to Ionomycin Treatment in a Skeletal Muscle Cell Line
title_full_unstemmed Broadband Electrical Spectroscopy to Distinguish Single-Cell Ca(2+) Changes Due to Ionomycin Treatment in a Skeletal Muscle Cell Line
title_short Broadband Electrical Spectroscopy to Distinguish Single-Cell Ca(2+) Changes Due to Ionomycin Treatment in a Skeletal Muscle Cell Line
title_sort broadband electrical spectroscopy to distinguish single-cell ca(2+) changes due to ionomycin treatment in a skeletal muscle cell line
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10181519/
https://www.ncbi.nlm.nih.gov/pubmed/37177559
http://dx.doi.org/10.3390/s23094358
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