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Estimating Fluor Emission Spectra Using Digital Image Analysis Compared to Spectrophotometer Measurements
This paper describes a practical method for obtaining the spectra of lights emitted by a fluor in a liquid scintillator (LS) using a digital camera. The emission wavelength results obtained using a digital image were compared with those obtained using a fluorescence spectrophotometer. For general us...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10181702/ https://www.ncbi.nlm.nih.gov/pubmed/37177494 http://dx.doi.org/10.3390/s23094291 |
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author | Park, Hyeon-Woo Choi, Ji-Won Joo, Kyung-Kwang Kim, Na-Ri Shin, Chang-Dong |
author_facet | Park, Hyeon-Woo Choi, Ji-Won Joo, Kyung-Kwang Kim, Na-Ri Shin, Chang-Dong |
author_sort | Park, Hyeon-Woo |
collection | PubMed |
description | This paper describes a practical method for obtaining the spectra of lights emitted by a fluor in a liquid scintillator (LS) using a digital camera. The emission wavelength results obtained using a digital image were compared with those obtained using a fluorescence spectrophotometer. For general users, conventional spectrophotometers are expensive and difficult to access. Moreover, their experimental measurement setup and processes are highly complicated, and they require considerable care in handling. To overcome these limitations, a feasibility study was performed to obtain the emission spectrum through image analysis. Specifically, the emission spectrum of a fluor dissolved in a liquid scintillator was obtained using digital image analysis. An image processing method was employed to convert the light irradiated during camera exposure into wavelengths. Hue (H) and wavelength (W) are closely related. Thus, we obtained an H-W response curve in the 400~450 nm wavelength region, using a light-emitting diode. Another relevant advantage of the method described in this study is its non-invasiveness in sealed LS samples. Our results showed that this method has the potential to accurately investigate the emission wavelengths of fluor within acceptable uncertainties. We envision the use of this method to perform experiments in chemistry and physics laboratories in the future. |
format | Online Article Text |
id | pubmed-10181702 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101817022023-05-13 Estimating Fluor Emission Spectra Using Digital Image Analysis Compared to Spectrophotometer Measurements Park, Hyeon-Woo Choi, Ji-Won Joo, Kyung-Kwang Kim, Na-Ri Shin, Chang-Dong Sensors (Basel) Article This paper describes a practical method for obtaining the spectra of lights emitted by a fluor in a liquid scintillator (LS) using a digital camera. The emission wavelength results obtained using a digital image were compared with those obtained using a fluorescence spectrophotometer. For general users, conventional spectrophotometers are expensive and difficult to access. Moreover, their experimental measurement setup and processes are highly complicated, and they require considerable care in handling. To overcome these limitations, a feasibility study was performed to obtain the emission spectrum through image analysis. Specifically, the emission spectrum of a fluor dissolved in a liquid scintillator was obtained using digital image analysis. An image processing method was employed to convert the light irradiated during camera exposure into wavelengths. Hue (H) and wavelength (W) are closely related. Thus, we obtained an H-W response curve in the 400~450 nm wavelength region, using a light-emitting diode. Another relevant advantage of the method described in this study is its non-invasiveness in sealed LS samples. Our results showed that this method has the potential to accurately investigate the emission wavelengths of fluor within acceptable uncertainties. We envision the use of this method to perform experiments in chemistry and physics laboratories in the future. MDPI 2023-04-26 /pmc/articles/PMC10181702/ /pubmed/37177494 http://dx.doi.org/10.3390/s23094291 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Park, Hyeon-Woo Choi, Ji-Won Joo, Kyung-Kwang Kim, Na-Ri Shin, Chang-Dong Estimating Fluor Emission Spectra Using Digital Image Analysis Compared to Spectrophotometer Measurements |
title | Estimating Fluor Emission Spectra Using Digital Image Analysis Compared to Spectrophotometer Measurements |
title_full | Estimating Fluor Emission Spectra Using Digital Image Analysis Compared to Spectrophotometer Measurements |
title_fullStr | Estimating Fluor Emission Spectra Using Digital Image Analysis Compared to Spectrophotometer Measurements |
title_full_unstemmed | Estimating Fluor Emission Spectra Using Digital Image Analysis Compared to Spectrophotometer Measurements |
title_short | Estimating Fluor Emission Spectra Using Digital Image Analysis Compared to Spectrophotometer Measurements |
title_sort | estimating fluor emission spectra using digital image analysis compared to spectrophotometer measurements |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10181702/ https://www.ncbi.nlm.nih.gov/pubmed/37177494 http://dx.doi.org/10.3390/s23094291 |
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