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Design and Validation of Experimental Setup for Cell Spheroid Radiofrequency-Induced Heating

While hyperthermia has been shown to induce a variety of cytotoxic and sensitizing effects on cancer tissues, the thermal dose–effect relationship is still not well quantified, and it is still unclear how it can be optimally combined with other treatment modalities. Additionally, it is speculated th...

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Autores principales: Androulakis, Ioannis, Ferrero, Riccardo, van Oossanen, Rogier, Manzin, Alessandra, Denkova, Antonia G., Djanashvili, Kristina, Nadar, Robin, van Rhoon, Gerard C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10181764/
https://www.ncbi.nlm.nih.gov/pubmed/37177718
http://dx.doi.org/10.3390/s23094514
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author Androulakis, Ioannis
Ferrero, Riccardo
van Oossanen, Rogier
Manzin, Alessandra
Denkova, Antonia G.
Djanashvili, Kristina
Nadar, Robin
van Rhoon, Gerard C.
author_facet Androulakis, Ioannis
Ferrero, Riccardo
van Oossanen, Rogier
Manzin, Alessandra
Denkova, Antonia G.
Djanashvili, Kristina
Nadar, Robin
van Rhoon, Gerard C.
author_sort Androulakis, Ioannis
collection PubMed
description While hyperthermia has been shown to induce a variety of cytotoxic and sensitizing effects on cancer tissues, the thermal dose–effect relationship is still not well quantified, and it is still unclear how it can be optimally combined with other treatment modalities. Additionally, it is speculated that different methods of applying hyperthermia, such as water bath heating or electromagnetic energy, may have an effect on the resulting biological mechanisms involved in cell death or in sensitizing tumor cells to other oncological treatments. In order to further quantify and characterize hyperthermia treatments on a cellular level, in vitro experiments shifted towards the use of 3D cell spheroids. These are in fact considered a more representative model of the cell environment when compared to 2D cell cultures. In order to perform radiofrequency (RF)-induced heating in vitro, we have recently developed a dedicated electromagnetic field applicator. In this study, using this applicator, we designed and validated an experimental setup which can heat 3D cell spheroids in a conical polypropylene vial, thus providing a reliable instrument for investigating hyperthermia effects at the cellular scale.
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spelling pubmed-101817642023-05-13 Design and Validation of Experimental Setup for Cell Spheroid Radiofrequency-Induced Heating Androulakis, Ioannis Ferrero, Riccardo van Oossanen, Rogier Manzin, Alessandra Denkova, Antonia G. Djanashvili, Kristina Nadar, Robin van Rhoon, Gerard C. Sensors (Basel) Article While hyperthermia has been shown to induce a variety of cytotoxic and sensitizing effects on cancer tissues, the thermal dose–effect relationship is still not well quantified, and it is still unclear how it can be optimally combined with other treatment modalities. Additionally, it is speculated that different methods of applying hyperthermia, such as water bath heating or electromagnetic energy, may have an effect on the resulting biological mechanisms involved in cell death or in sensitizing tumor cells to other oncological treatments. In order to further quantify and characterize hyperthermia treatments on a cellular level, in vitro experiments shifted towards the use of 3D cell spheroids. These are in fact considered a more representative model of the cell environment when compared to 2D cell cultures. In order to perform radiofrequency (RF)-induced heating in vitro, we have recently developed a dedicated electromagnetic field applicator. In this study, using this applicator, we designed and validated an experimental setup which can heat 3D cell spheroids in a conical polypropylene vial, thus providing a reliable instrument for investigating hyperthermia effects at the cellular scale. MDPI 2023-05-05 /pmc/articles/PMC10181764/ /pubmed/37177718 http://dx.doi.org/10.3390/s23094514 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Androulakis, Ioannis
Ferrero, Riccardo
van Oossanen, Rogier
Manzin, Alessandra
Denkova, Antonia G.
Djanashvili, Kristina
Nadar, Robin
van Rhoon, Gerard C.
Design and Validation of Experimental Setup for Cell Spheroid Radiofrequency-Induced Heating
title Design and Validation of Experimental Setup for Cell Spheroid Radiofrequency-Induced Heating
title_full Design and Validation of Experimental Setup for Cell Spheroid Radiofrequency-Induced Heating
title_fullStr Design and Validation of Experimental Setup for Cell Spheroid Radiofrequency-Induced Heating
title_full_unstemmed Design and Validation of Experimental Setup for Cell Spheroid Radiofrequency-Induced Heating
title_short Design and Validation of Experimental Setup for Cell Spheroid Radiofrequency-Induced Heating
title_sort design and validation of experimental setup for cell spheroid radiofrequency-induced heating
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10181764/
https://www.ncbi.nlm.nih.gov/pubmed/37177718
http://dx.doi.org/10.3390/s23094514
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