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Immunoengineered MXene nanosystem for mitigation of alloantigen presentation and prevention of transplant vasculopathy

MXenes are an emerging class of nanomaterials with significant potential for applications in nanomedicine. Amongst MXene technologies, titanium carbide (Ti(3)C(2)T(x)) nanomaterials are the most mature and have received significant attention to tackle longstanding clinical challenges due to its tail...

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Autores principales: Yan, Weiang, Rafieerad, Alireza, Alagarsamy, Keshav Narayan, Saleth, Leena Regi, Arora, Rakesh C., Dhingra, Sanjiv
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Ltd 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10181944/
https://www.ncbi.nlm.nih.gov/pubmed/37187503
http://dx.doi.org/10.1016/j.nantod.2022.101706
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author Yan, Weiang
Rafieerad, Alireza
Alagarsamy, Keshav Narayan
Saleth, Leena Regi
Arora, Rakesh C.
Dhingra, Sanjiv
author_facet Yan, Weiang
Rafieerad, Alireza
Alagarsamy, Keshav Narayan
Saleth, Leena Regi
Arora, Rakesh C.
Dhingra, Sanjiv
author_sort Yan, Weiang
collection PubMed
description MXenes are an emerging class of nanomaterials with significant potential for applications in nanomedicine. Amongst MXene technologies, titanium carbide (Ti(3)C(2)T(x)) nanomaterials are the most mature and have received significant attention to tackle longstanding clinical challenges due to its tailored physical and material properties. Cardiac allograft vasculopathy is an aggressive form of atherosclerosis and a major cause of mortality among patients with heart transplants. Blood vessel endothelial cells (ECs) stimulate alloreactive T-lymphocytes to result in sustained inflammation. Herein, we report the first application of Ti(3)C(2)T(x) MXene nanosheets for prevention of allograft vasculopathy. MXene nanosheets interacted with human ECs and downregulated the expression of genes involved in alloantigen presentation, and consequently reduced the activation of allogeneic lymphocytes. RNA-Seq analysis of lymphocytes showed that treatment with MXene downregulated genes responsible for transplant-induced T-cell activation, cell-mediated rejection, and development of allograft vasculopathy. In an in vivo rat model of allograft vasculopathy, treatment with MXene reduced lymphocyte infiltration and preserved medial smooth muscle cell integrity within transplanted aortic allografts. These findings highlight the potential of Ti(3)C(2)T(x) MXene in treatment of allograft vasculopathy and inflammatory diseases.
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spelling pubmed-101819442023-05-14 Immunoengineered MXene nanosystem for mitigation of alloantigen presentation and prevention of transplant vasculopathy Yan, Weiang Rafieerad, Alireza Alagarsamy, Keshav Narayan Saleth, Leena Regi Arora, Rakesh C. Dhingra, Sanjiv Nano Today Article MXenes are an emerging class of nanomaterials with significant potential for applications in nanomedicine. Amongst MXene technologies, titanium carbide (Ti(3)C(2)T(x)) nanomaterials are the most mature and have received significant attention to tackle longstanding clinical challenges due to its tailored physical and material properties. Cardiac allograft vasculopathy is an aggressive form of atherosclerosis and a major cause of mortality among patients with heart transplants. Blood vessel endothelial cells (ECs) stimulate alloreactive T-lymphocytes to result in sustained inflammation. Herein, we report the first application of Ti(3)C(2)T(x) MXene nanosheets for prevention of allograft vasculopathy. MXene nanosheets interacted with human ECs and downregulated the expression of genes involved in alloantigen presentation, and consequently reduced the activation of allogeneic lymphocytes. RNA-Seq analysis of lymphocytes showed that treatment with MXene downregulated genes responsible for transplant-induced T-cell activation, cell-mediated rejection, and development of allograft vasculopathy. In an in vivo rat model of allograft vasculopathy, treatment with MXene reduced lymphocyte infiltration and preserved medial smooth muscle cell integrity within transplanted aortic allografts. These findings highlight the potential of Ti(3)C(2)T(x) MXene in treatment of allograft vasculopathy and inflammatory diseases. Elsevier Ltd 2023-02 /pmc/articles/PMC10181944/ /pubmed/37187503 http://dx.doi.org/10.1016/j.nantod.2022.101706 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yan, Weiang
Rafieerad, Alireza
Alagarsamy, Keshav Narayan
Saleth, Leena Regi
Arora, Rakesh C.
Dhingra, Sanjiv
Immunoengineered MXene nanosystem for mitigation of alloantigen presentation and prevention of transplant vasculopathy
title Immunoengineered MXene nanosystem for mitigation of alloantigen presentation and prevention of transplant vasculopathy
title_full Immunoengineered MXene nanosystem for mitigation of alloantigen presentation and prevention of transplant vasculopathy
title_fullStr Immunoengineered MXene nanosystem for mitigation of alloantigen presentation and prevention of transplant vasculopathy
title_full_unstemmed Immunoengineered MXene nanosystem for mitigation of alloantigen presentation and prevention of transplant vasculopathy
title_short Immunoengineered MXene nanosystem for mitigation of alloantigen presentation and prevention of transplant vasculopathy
title_sort immunoengineered mxene nanosystem for mitigation of alloantigen presentation and prevention of transplant vasculopathy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10181944/
https://www.ncbi.nlm.nih.gov/pubmed/37187503
http://dx.doi.org/10.1016/j.nantod.2022.101706
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