Cargando…
Immunoengineered MXene nanosystem for mitigation of alloantigen presentation and prevention of transplant vasculopathy
MXenes are an emerging class of nanomaterials with significant potential for applications in nanomedicine. Amongst MXene technologies, titanium carbide (Ti(3)C(2)T(x)) nanomaterials are the most mature and have received significant attention to tackle longstanding clinical challenges due to its tail...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Ltd
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10181944/ https://www.ncbi.nlm.nih.gov/pubmed/37187503 http://dx.doi.org/10.1016/j.nantod.2022.101706 |
_version_ | 1785041684476723200 |
---|---|
author | Yan, Weiang Rafieerad, Alireza Alagarsamy, Keshav Narayan Saleth, Leena Regi Arora, Rakesh C. Dhingra, Sanjiv |
author_facet | Yan, Weiang Rafieerad, Alireza Alagarsamy, Keshav Narayan Saleth, Leena Regi Arora, Rakesh C. Dhingra, Sanjiv |
author_sort | Yan, Weiang |
collection | PubMed |
description | MXenes are an emerging class of nanomaterials with significant potential for applications in nanomedicine. Amongst MXene technologies, titanium carbide (Ti(3)C(2)T(x)) nanomaterials are the most mature and have received significant attention to tackle longstanding clinical challenges due to its tailored physical and material properties. Cardiac allograft vasculopathy is an aggressive form of atherosclerosis and a major cause of mortality among patients with heart transplants. Blood vessel endothelial cells (ECs) stimulate alloreactive T-lymphocytes to result in sustained inflammation. Herein, we report the first application of Ti(3)C(2)T(x) MXene nanosheets for prevention of allograft vasculopathy. MXene nanosheets interacted with human ECs and downregulated the expression of genes involved in alloantigen presentation, and consequently reduced the activation of allogeneic lymphocytes. RNA-Seq analysis of lymphocytes showed that treatment with MXene downregulated genes responsible for transplant-induced T-cell activation, cell-mediated rejection, and development of allograft vasculopathy. In an in vivo rat model of allograft vasculopathy, treatment with MXene reduced lymphocyte infiltration and preserved medial smooth muscle cell integrity within transplanted aortic allografts. These findings highlight the potential of Ti(3)C(2)T(x) MXene in treatment of allograft vasculopathy and inflammatory diseases. |
format | Online Article Text |
id | pubmed-10181944 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-101819442023-05-14 Immunoengineered MXene nanosystem for mitigation of alloantigen presentation and prevention of transplant vasculopathy Yan, Weiang Rafieerad, Alireza Alagarsamy, Keshav Narayan Saleth, Leena Regi Arora, Rakesh C. Dhingra, Sanjiv Nano Today Article MXenes are an emerging class of nanomaterials with significant potential for applications in nanomedicine. Amongst MXene technologies, titanium carbide (Ti(3)C(2)T(x)) nanomaterials are the most mature and have received significant attention to tackle longstanding clinical challenges due to its tailored physical and material properties. Cardiac allograft vasculopathy is an aggressive form of atherosclerosis and a major cause of mortality among patients with heart transplants. Blood vessel endothelial cells (ECs) stimulate alloreactive T-lymphocytes to result in sustained inflammation. Herein, we report the first application of Ti(3)C(2)T(x) MXene nanosheets for prevention of allograft vasculopathy. MXene nanosheets interacted with human ECs and downregulated the expression of genes involved in alloantigen presentation, and consequently reduced the activation of allogeneic lymphocytes. RNA-Seq analysis of lymphocytes showed that treatment with MXene downregulated genes responsible for transplant-induced T-cell activation, cell-mediated rejection, and development of allograft vasculopathy. In an in vivo rat model of allograft vasculopathy, treatment with MXene reduced lymphocyte infiltration and preserved medial smooth muscle cell integrity within transplanted aortic allografts. These findings highlight the potential of Ti(3)C(2)T(x) MXene in treatment of allograft vasculopathy and inflammatory diseases. Elsevier Ltd 2023-02 /pmc/articles/PMC10181944/ /pubmed/37187503 http://dx.doi.org/10.1016/j.nantod.2022.101706 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yan, Weiang Rafieerad, Alireza Alagarsamy, Keshav Narayan Saleth, Leena Regi Arora, Rakesh C. Dhingra, Sanjiv Immunoengineered MXene nanosystem for mitigation of alloantigen presentation and prevention of transplant vasculopathy |
title | Immunoengineered MXene nanosystem for mitigation of alloantigen presentation and prevention of transplant vasculopathy |
title_full | Immunoengineered MXene nanosystem for mitigation of alloantigen presentation and prevention of transplant vasculopathy |
title_fullStr | Immunoengineered MXene nanosystem for mitigation of alloantigen presentation and prevention of transplant vasculopathy |
title_full_unstemmed | Immunoengineered MXene nanosystem for mitigation of alloantigen presentation and prevention of transplant vasculopathy |
title_short | Immunoengineered MXene nanosystem for mitigation of alloantigen presentation and prevention of transplant vasculopathy |
title_sort | immunoengineered mxene nanosystem for mitigation of alloantigen presentation and prevention of transplant vasculopathy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10181944/ https://www.ncbi.nlm.nih.gov/pubmed/37187503 http://dx.doi.org/10.1016/j.nantod.2022.101706 |
work_keys_str_mv | AT yanweiang immunoengineeredmxenenanosystemformitigationofalloantigenpresentationandpreventionoftransplantvasculopathy AT rafieeradalireza immunoengineeredmxenenanosystemformitigationofalloantigenpresentationandpreventionoftransplantvasculopathy AT alagarsamykeshavnarayan immunoengineeredmxenenanosystemformitigationofalloantigenpresentationandpreventionoftransplantvasculopathy AT salethleenaregi immunoengineeredmxenenanosystemformitigationofalloantigenpresentationandpreventionoftransplantvasculopathy AT arorarakeshc immunoengineeredmxenenanosystemformitigationofalloantigenpresentationandpreventionoftransplantvasculopathy AT dhingrasanjiv immunoengineeredmxenenanosystemformitigationofalloantigenpresentationandpreventionoftransplantvasculopathy |