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Taurine Promotes Differentiation and Maturation of Neural Stem/Progenitor Cells from the Subventricular Zone via Activation of GABA(A) Receptors

Neurogenesis, the formation of new neurons in the brain, occurs throughout the lifespan in the subgranular zone of the dentate gyrus and subventricular zone (SVZ) lining the lateral ventricles of the mammal brain. In this process, gamma-aminobutyric acid (GABA) and its ionotropic receptor, the GABA(...

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Autores principales: Gutiérrez-Castañeda, Nadia Estefanía, González-Corona, Jessica, Griego, Ernesto, Galván, Emilio J., Ochoa-de la Paz, Lenin David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10181976/
https://www.ncbi.nlm.nih.gov/pubmed/36862323
http://dx.doi.org/10.1007/s11064-023-03883-2
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author Gutiérrez-Castañeda, Nadia Estefanía
González-Corona, Jessica
Griego, Ernesto
Galván, Emilio J.
Ochoa-de la Paz, Lenin David
author_facet Gutiérrez-Castañeda, Nadia Estefanía
González-Corona, Jessica
Griego, Ernesto
Galván, Emilio J.
Ochoa-de la Paz, Lenin David
author_sort Gutiérrez-Castañeda, Nadia Estefanía
collection PubMed
description Neurogenesis, the formation of new neurons in the brain, occurs throughout the lifespan in the subgranular zone of the dentate gyrus and subventricular zone (SVZ) lining the lateral ventricles of the mammal brain. In this process, gamma-aminobutyric acid (GABA) and its ionotropic receptor, the GABA(A) receptor (GABA(A)R), play a critical role in the proliferation, differentiation, and migration process of neural stem/progenitor cells (NPC). Taurine, a non-essential amino acid widely distributed throughout the central nervous system, increases the proliferation of SVZ progenitor cells by a mechanism that may involve GABA(A)R activation. Therefore, we characterized the effects of taurine on the differentiation process of NPC expressing GABA(A)R. Preincubation of NPC-SVZ with taurine increased microtubule-stabilizing proteins assessed with the doublecortin assay. Taurine, like GABA, stimulated a neuronal-like morphology of NPC-SVZ and increased the number and length of primary, secondary, and tertiary neurites compared with control NPC of the SVZ. Furthermore, neurite outgrowth was prevented when simultaneously incubating cells with taurine or GABA and the GABA(A)R blocker, picrotoxin. Patch-clamp recordings revealed a series of modifications in the NPCs’ passive and active electrophysiological properties exposed to taurine, including regenerative spikes with kinetic properties similar to the action potentials of functional neurons. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11064-023-03883-2.
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spelling pubmed-101819762023-05-14 Taurine Promotes Differentiation and Maturation of Neural Stem/Progenitor Cells from the Subventricular Zone via Activation of GABA(A) Receptors Gutiérrez-Castañeda, Nadia Estefanía González-Corona, Jessica Griego, Ernesto Galván, Emilio J. Ochoa-de la Paz, Lenin David Neurochem Res Original Paper Neurogenesis, the formation of new neurons in the brain, occurs throughout the lifespan in the subgranular zone of the dentate gyrus and subventricular zone (SVZ) lining the lateral ventricles of the mammal brain. In this process, gamma-aminobutyric acid (GABA) and its ionotropic receptor, the GABA(A) receptor (GABA(A)R), play a critical role in the proliferation, differentiation, and migration process of neural stem/progenitor cells (NPC). Taurine, a non-essential amino acid widely distributed throughout the central nervous system, increases the proliferation of SVZ progenitor cells by a mechanism that may involve GABA(A)R activation. Therefore, we characterized the effects of taurine on the differentiation process of NPC expressing GABA(A)R. Preincubation of NPC-SVZ with taurine increased microtubule-stabilizing proteins assessed with the doublecortin assay. Taurine, like GABA, stimulated a neuronal-like morphology of NPC-SVZ and increased the number and length of primary, secondary, and tertiary neurites compared with control NPC of the SVZ. Furthermore, neurite outgrowth was prevented when simultaneously incubating cells with taurine or GABA and the GABA(A)R blocker, picrotoxin. Patch-clamp recordings revealed a series of modifications in the NPCs’ passive and active electrophysiological properties exposed to taurine, including regenerative spikes with kinetic properties similar to the action potentials of functional neurons. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11064-023-03883-2. Springer US 2023-03-02 2023 /pmc/articles/PMC10181976/ /pubmed/36862323 http://dx.doi.org/10.1007/s11064-023-03883-2 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Paper
Gutiérrez-Castañeda, Nadia Estefanía
González-Corona, Jessica
Griego, Ernesto
Galván, Emilio J.
Ochoa-de la Paz, Lenin David
Taurine Promotes Differentiation and Maturation of Neural Stem/Progenitor Cells from the Subventricular Zone via Activation of GABA(A) Receptors
title Taurine Promotes Differentiation and Maturation of Neural Stem/Progenitor Cells from the Subventricular Zone via Activation of GABA(A) Receptors
title_full Taurine Promotes Differentiation and Maturation of Neural Stem/Progenitor Cells from the Subventricular Zone via Activation of GABA(A) Receptors
title_fullStr Taurine Promotes Differentiation and Maturation of Neural Stem/Progenitor Cells from the Subventricular Zone via Activation of GABA(A) Receptors
title_full_unstemmed Taurine Promotes Differentiation and Maturation of Neural Stem/Progenitor Cells from the Subventricular Zone via Activation of GABA(A) Receptors
title_short Taurine Promotes Differentiation and Maturation of Neural Stem/Progenitor Cells from the Subventricular Zone via Activation of GABA(A) Receptors
title_sort taurine promotes differentiation and maturation of neural stem/progenitor cells from the subventricular zone via activation of gaba(a) receptors
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10181976/
https://www.ncbi.nlm.nih.gov/pubmed/36862323
http://dx.doi.org/10.1007/s11064-023-03883-2
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