Cargando…

Screening, identification and experimental design to optimization of the selenite bioremediation by new isolated Bacillus sp. Selena 3 in water

BACKGROUND AND OBJECTIVES: Heavy metals pollution is one of the most important concerns in the world. Selenium is one of the most important elements for the life, but if the absorption of this element in cells increases, it acts as a toxic element. MATERIALS AND METHODS: In this study, bacterial iso...

Descripción completa

Detalles Bibliográficos
Autores principales: Tavafi, Hadis, Tajer-Mohammad-Ghazvini, Parisa, Babaei, Arash
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10183071/
https://www.ncbi.nlm.nih.gov/pubmed/37193228
http://dx.doi.org/10.18502/ijm.v15i2.12481
_version_ 1785041877386395648
author Tavafi, Hadis
Tajer-Mohammad-Ghazvini, Parisa
Babaei, Arash
author_facet Tavafi, Hadis
Tajer-Mohammad-Ghazvini, Parisa
Babaei, Arash
author_sort Tavafi, Hadis
collection PubMed
description BACKGROUND AND OBJECTIVES: Heavy metals pollution is one of the most important concerns in the world. Selenium is one of the most important elements for the life, but if the absorption of this element in cells increases, it acts as a toxic element. MATERIALS AND METHODS: In this study, bacterial isolates were screened and isolated from selenium-contaminated soil and water. Twenty-five out of 42 isolates were able to reduce Selenite. Also, the response surface method (RSM) was used to evaluate and optimize the biological reduction of selenite by Selena 3. Factors of bacterial inoculation percentage, time, and amount of selenium oxyanion salt concentration were studied at five levels of −α, −1, 0, +1, and +α. RESULTS: Bacillus sp. Selena 3 was able to reduce 80 mM sodium selenite in less than 4 hours compared to other bacterial isolates. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of sodium selenite Bacillus sp. Selena 3 was reported as 160 and 320 mM, respectively. The results showed that with increasing duration, the percentage of selenite reduction by bacteria increases and the percentage of bacterial inoculation does not have much effect on its reduction. CONCLUSION: Due to the ability of Bacillus sp. Selena 3 for rapid reduction in significant concentration of selenium oxyanion (SeO(3)(2−)), this bacterium can be used as an efficient candidate in removing selenite from the environment.
format Online
Article
Text
id pubmed-10183071
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Tehran University of Medical Sciences
record_format MEDLINE/PubMed
spelling pubmed-101830712023-05-15 Screening, identification and experimental design to optimization of the selenite bioremediation by new isolated Bacillus sp. Selena 3 in water Tavafi, Hadis Tajer-Mohammad-Ghazvini, Parisa Babaei, Arash Iran J Microbiol Original Article BACKGROUND AND OBJECTIVES: Heavy metals pollution is one of the most important concerns in the world. Selenium is one of the most important elements for the life, but if the absorption of this element in cells increases, it acts as a toxic element. MATERIALS AND METHODS: In this study, bacterial isolates were screened and isolated from selenium-contaminated soil and water. Twenty-five out of 42 isolates were able to reduce Selenite. Also, the response surface method (RSM) was used to evaluate and optimize the biological reduction of selenite by Selena 3. Factors of bacterial inoculation percentage, time, and amount of selenium oxyanion salt concentration were studied at five levels of −α, −1, 0, +1, and +α. RESULTS: Bacillus sp. Selena 3 was able to reduce 80 mM sodium selenite in less than 4 hours compared to other bacterial isolates. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of sodium selenite Bacillus sp. Selena 3 was reported as 160 and 320 mM, respectively. The results showed that with increasing duration, the percentage of selenite reduction by bacteria increases and the percentage of bacterial inoculation does not have much effect on its reduction. CONCLUSION: Due to the ability of Bacillus sp. Selena 3 for rapid reduction in significant concentration of selenium oxyanion (SeO(3)(2−)), this bacterium can be used as an efficient candidate in removing selenite from the environment. Tehran University of Medical Sciences 2023-04 /pmc/articles/PMC10183071/ /pubmed/37193228 http://dx.doi.org/10.18502/ijm.v15i2.12481 Text en Copyright © 2023 The Authors. Published by Tehran University of Medical Sciences https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International license (https://creativecommons.org/licenses/by-nc/4.0/). Non-commercial uses of the work are permitted, provided the original work is properly cited.
spellingShingle Original Article
Tavafi, Hadis
Tajer-Mohammad-Ghazvini, Parisa
Babaei, Arash
Screening, identification and experimental design to optimization of the selenite bioremediation by new isolated Bacillus sp. Selena 3 in water
title Screening, identification and experimental design to optimization of the selenite bioremediation by new isolated Bacillus sp. Selena 3 in water
title_full Screening, identification and experimental design to optimization of the selenite bioremediation by new isolated Bacillus sp. Selena 3 in water
title_fullStr Screening, identification and experimental design to optimization of the selenite bioremediation by new isolated Bacillus sp. Selena 3 in water
title_full_unstemmed Screening, identification and experimental design to optimization of the selenite bioremediation by new isolated Bacillus sp. Selena 3 in water
title_short Screening, identification and experimental design to optimization of the selenite bioremediation by new isolated Bacillus sp. Selena 3 in water
title_sort screening, identification and experimental design to optimization of the selenite bioremediation by new isolated bacillus sp. selena 3 in water
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10183071/
https://www.ncbi.nlm.nih.gov/pubmed/37193228
http://dx.doi.org/10.18502/ijm.v15i2.12481
work_keys_str_mv AT tavafihadis screeningidentificationandexperimentaldesigntooptimizationoftheselenitebioremediationbynewisolatedbacillusspselena3inwater
AT tajermohammadghazviniparisa screeningidentificationandexperimentaldesigntooptimizationoftheselenitebioremediationbynewisolatedbacillusspselena3inwater
AT babaeiarash screeningidentificationandexperimentaldesigntooptimizationoftheselenitebioremediationbynewisolatedbacillusspselena3inwater