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Isolation and identification of microbiota of Culex quinquefasciatus for their application as paratransgenic tools in vector control
BACKGROUND AND OBJECTIVES: Although the study on the bacteria residing in the mid-gut, salivary gland, and reproductive organs of insect vectors have drawn appeal to the host-pathogen interactions, we know comparatively less about microbiota that naturally exist in different mosquito organs within I...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Tehran University of Medical Sciences
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10183081/ https://www.ncbi.nlm.nih.gov/pubmed/37193245 http://dx.doi.org/10.18502/ijm.v15i2.12478 |
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author | Nourani, Leila Raz, Abbasali Djadid, Navid Dinparast |
author_facet | Nourani, Leila Raz, Abbasali Djadid, Navid Dinparast |
author_sort | Nourani, Leila |
collection | PubMed |
description | BACKGROUND AND OBJECTIVES: Although the study on the bacteria residing in the mid-gut, salivary gland, and reproductive organs of insect vectors have drawn appeal to the host-pathogen interactions, we know comparatively less about microbiota that naturally exist in different mosquito organs within Iran. MATERIALS AND METHODS: In the current investigation, PCR assay by using 16S rRNA gene amplification and DNA sequencing, in addition to the traditional culture-based approach utilized for the detection of cultivable bacterial assemblages in mid-gut and reproductive tracts of Culex quinquefasciatus. RESULTS: The identified bacteria isolated from different tissues of 45 individuals were consisted of Achromobacter, Aeromonas, Arthrobacter, Asaia, Enterobacter, Gluconobacter, Klebsiella, Lysinibacillus, Micrococcus, Psuedomonas and Serratia. The results showed that Proteobacteria was the most prevalent phylum in both genders' mid-gut and reproductive tracts, and Asaia was the most common bacteria that originated in adult females and males’ tissues. CONCLUSION: These outcomes recommend that the discovered microbiome may span through Cx. quinquefasciatus populations. This data can be utilized to interfere with the transmission of pathogens and design new strategies for the control of mosquito-borne diseases. |
format | Online Article Text |
id | pubmed-10183081 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Tehran University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-101830812023-05-15 Isolation and identification of microbiota of Culex quinquefasciatus for their application as paratransgenic tools in vector control Nourani, Leila Raz, Abbasali Djadid, Navid Dinparast Iran J Microbiol Original Article BACKGROUND AND OBJECTIVES: Although the study on the bacteria residing in the mid-gut, salivary gland, and reproductive organs of insect vectors have drawn appeal to the host-pathogen interactions, we know comparatively less about microbiota that naturally exist in different mosquito organs within Iran. MATERIALS AND METHODS: In the current investigation, PCR assay by using 16S rRNA gene amplification and DNA sequencing, in addition to the traditional culture-based approach utilized for the detection of cultivable bacterial assemblages in mid-gut and reproductive tracts of Culex quinquefasciatus. RESULTS: The identified bacteria isolated from different tissues of 45 individuals were consisted of Achromobacter, Aeromonas, Arthrobacter, Asaia, Enterobacter, Gluconobacter, Klebsiella, Lysinibacillus, Micrococcus, Psuedomonas and Serratia. The results showed that Proteobacteria was the most prevalent phylum in both genders' mid-gut and reproductive tracts, and Asaia was the most common bacteria that originated in adult females and males’ tissues. CONCLUSION: These outcomes recommend that the discovered microbiome may span through Cx. quinquefasciatus populations. This data can be utilized to interfere with the transmission of pathogens and design new strategies for the control of mosquito-borne diseases. Tehran University of Medical Sciences 2023-04 /pmc/articles/PMC10183081/ /pubmed/37193245 http://dx.doi.org/10.18502/ijm.v15i2.12478 Text en Copyright © 2023 The Authors. Published by Tehran University of Medical Sciences https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International license (https://creativecommons.org/licenses/by-nc/4.0/). Non-commercial uses of the work are permitted, provided the original work is properly cited. |
spellingShingle | Original Article Nourani, Leila Raz, Abbasali Djadid, Navid Dinparast Isolation and identification of microbiota of Culex quinquefasciatus for their application as paratransgenic tools in vector control |
title | Isolation and identification of microbiota of Culex quinquefasciatus for their application as paratransgenic tools in vector control |
title_full | Isolation and identification of microbiota of Culex quinquefasciatus for their application as paratransgenic tools in vector control |
title_fullStr | Isolation and identification of microbiota of Culex quinquefasciatus for their application as paratransgenic tools in vector control |
title_full_unstemmed | Isolation and identification of microbiota of Culex quinquefasciatus for their application as paratransgenic tools in vector control |
title_short | Isolation and identification of microbiota of Culex quinquefasciatus for their application as paratransgenic tools in vector control |
title_sort | isolation and identification of microbiota of culex quinquefasciatus for their application as paratransgenic tools in vector control |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10183081/ https://www.ncbi.nlm.nih.gov/pubmed/37193245 http://dx.doi.org/10.18502/ijm.v15i2.12478 |
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