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Synthesis and Clinical Examination of Novel Formulations of Ivermectin, Albendazole and Niclosamide for the Treatment of Equine Gastrointestinal Helminthoses
BACKGROUND: This study aimed to develop new complex preparations of ivermectin, niclosamide and albendazole based on solid-phase mechanochemical technology, and to evaluate their efficacy against equine nematodosis and cestodosis. METHODS: Novel formulation of antiparasitic paste were prepared using...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Tehran University of Medical Sciences
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10183445/ https://www.ncbi.nlm.nih.gov/pubmed/37197070 http://dx.doi.org/10.18502/ijpa.v18i1.12381 |
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author | Marchenko, Victor Alexeevich Khalikov, Salavat Samadovich Biryukov, Ivan Vladimirovich Efremova, Elena Alexandrovna Ilyin, Mikhail Mikhailovich Sazmand, Alireza |
author_facet | Marchenko, Victor Alexeevich Khalikov, Salavat Samadovich Biryukov, Ivan Vladimirovich Efremova, Elena Alexandrovna Ilyin, Mikhail Mikhailovich Sazmand, Alireza |
author_sort | Marchenko, Victor Alexeevich |
collection | PubMed |
description | BACKGROUND: This study aimed to develop new complex preparations of ivermectin, niclosamide and albendazole based on solid-phase mechanochemical technology, and to evaluate their efficacy against equine nematodosis and cestodosis. METHODS: Novel formulation of antiparasitic paste were prepared using joint mechano-chemical treatment of ivermectin (0.2 mg/kg bodyweight; BW), niclosamide (10 mg/kg BW) and albendazole (3, 5, 10 mg/kg BW) substances with polyvinylpyrrolidone and arabinogalactan. For the evaluation of activity of different doses of formulations against gastrointestinal tract helminths a total of 151 adult horses of the Novoaltai breed weighing 450–500 kg naturally infected with strongyles (>150 egg per gram of faeces, EPG), Parascaris spp. (>20 EPG) and Anoplocephala spp. (>10 EPG) were selected. Antiparasitic pastes were orally fed to the horses and faecal egg count reduction counts were compared prior to and 14 days after the treatment. RESULTS: Pastes with mechanically modified ivermectin showed 91.4–100% efficacy against strongyles and Parascaris. Pastes with modified albendazole and niclosamide were also effective against Anoplocephala in all tested dosages i.e. 78.6–100%. In particular, treatment with two formulations containing i) 0.2 mg ivermectin, 10 mg albendazole, 10 mg niclosamide, and ii) 0.2 mg ivermectin, 3 mg albendazole showed 100% efficacy against strongyles, Parascaris and Anoplocephala. CONCLUSION: Solid-phase mechanochemical technology could be applied in equine anthelminthics production. It is suggested that future studies focus on plasma concentration-time profile of these highly effective pastes. |
format | Online Article Text |
id | pubmed-10183445 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Tehran University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-101834452023-05-16 Synthesis and Clinical Examination of Novel Formulations of Ivermectin, Albendazole and Niclosamide for the Treatment of Equine Gastrointestinal Helminthoses Marchenko, Victor Alexeevich Khalikov, Salavat Samadovich Biryukov, Ivan Vladimirovich Efremova, Elena Alexandrovna Ilyin, Mikhail Mikhailovich Sazmand, Alireza Iran J Parasitol Original Article BACKGROUND: This study aimed to develop new complex preparations of ivermectin, niclosamide and albendazole based on solid-phase mechanochemical technology, and to evaluate their efficacy against equine nematodosis and cestodosis. METHODS: Novel formulation of antiparasitic paste were prepared using joint mechano-chemical treatment of ivermectin (0.2 mg/kg bodyweight; BW), niclosamide (10 mg/kg BW) and albendazole (3, 5, 10 mg/kg BW) substances with polyvinylpyrrolidone and arabinogalactan. For the evaluation of activity of different doses of formulations against gastrointestinal tract helminths a total of 151 adult horses of the Novoaltai breed weighing 450–500 kg naturally infected with strongyles (>150 egg per gram of faeces, EPG), Parascaris spp. (>20 EPG) and Anoplocephala spp. (>10 EPG) were selected. Antiparasitic pastes were orally fed to the horses and faecal egg count reduction counts were compared prior to and 14 days after the treatment. RESULTS: Pastes with mechanically modified ivermectin showed 91.4–100% efficacy against strongyles and Parascaris. Pastes with modified albendazole and niclosamide were also effective against Anoplocephala in all tested dosages i.e. 78.6–100%. In particular, treatment with two formulations containing i) 0.2 mg ivermectin, 10 mg albendazole, 10 mg niclosamide, and ii) 0.2 mg ivermectin, 3 mg albendazole showed 100% efficacy against strongyles, Parascaris and Anoplocephala. CONCLUSION: Solid-phase mechanochemical technology could be applied in equine anthelminthics production. It is suggested that future studies focus on plasma concentration-time profile of these highly effective pastes. Tehran University of Medical Sciences 2023 /pmc/articles/PMC10183445/ /pubmed/37197070 http://dx.doi.org/10.18502/ijpa.v18i1.12381 Text en Copyright © 2023 Marchenko et al. Published by Tehran University of Medical Sciences https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International license (https://creativecommons.org/licenses/by-nc/4.0/). Non-commercial uses of the work are permitted, provided the original work is properly cited. |
spellingShingle | Original Article Marchenko, Victor Alexeevich Khalikov, Salavat Samadovich Biryukov, Ivan Vladimirovich Efremova, Elena Alexandrovna Ilyin, Mikhail Mikhailovich Sazmand, Alireza Synthesis and Clinical Examination of Novel Formulations of Ivermectin, Albendazole and Niclosamide for the Treatment of Equine Gastrointestinal Helminthoses |
title | Synthesis and Clinical Examination of Novel Formulations of Ivermectin, Albendazole and Niclosamide for the Treatment of Equine Gastrointestinal Helminthoses |
title_full | Synthesis and Clinical Examination of Novel Formulations of Ivermectin, Albendazole and Niclosamide for the Treatment of Equine Gastrointestinal Helminthoses |
title_fullStr | Synthesis and Clinical Examination of Novel Formulations of Ivermectin, Albendazole and Niclosamide for the Treatment of Equine Gastrointestinal Helminthoses |
title_full_unstemmed | Synthesis and Clinical Examination of Novel Formulations of Ivermectin, Albendazole and Niclosamide for the Treatment of Equine Gastrointestinal Helminthoses |
title_short | Synthesis and Clinical Examination of Novel Formulations of Ivermectin, Albendazole and Niclosamide for the Treatment of Equine Gastrointestinal Helminthoses |
title_sort | synthesis and clinical examination of novel formulations of ivermectin, albendazole and niclosamide for the treatment of equine gastrointestinal helminthoses |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10183445/ https://www.ncbi.nlm.nih.gov/pubmed/37197070 http://dx.doi.org/10.18502/ijpa.v18i1.12381 |
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