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The thymus and T-cell ontogeny in ballan wrasse (Labrus bergylta) is nutritionally modelled

Marine fish larvae often experience high mortality unrelated to predation during early life stages, and farmed ballan wrasse (Labrus bergylta) is no exception. Knowing when the adaptive immune system is developed and fully functional, and how nutrition may modulate these processes is therefore of im...

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Autores principales: Etayo, Angela, Lie, Kai K., Bjelland, Reidun M., Hordvik, Ivar, Øvergård, Aina-Cathrine, Sæle, Øystein
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10183603/
https://www.ncbi.nlm.nih.gov/pubmed/37197651
http://dx.doi.org/10.3389/fimmu.2023.1166785
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author Etayo, Angela
Lie, Kai K.
Bjelland, Reidun M.
Hordvik, Ivar
Øvergård, Aina-Cathrine
Sæle, Øystein
author_facet Etayo, Angela
Lie, Kai K.
Bjelland, Reidun M.
Hordvik, Ivar
Øvergård, Aina-Cathrine
Sæle, Øystein
author_sort Etayo, Angela
collection PubMed
description Marine fish larvae often experience high mortality unrelated to predation during early life stages, and farmed ballan wrasse (Labrus bergylta) is no exception. Knowing when the adaptive immune system is developed and fully functional, and how nutrition may modulate these processes is therefore of importance to establish effective prophylactic measures and will also extend the relatively limited knowledge on the immune system in lower vertebrates. The thymus anlage of ballan wrasse was found to be histologically visible for the first time at larval stage 3 (20–30 days post hatch, dph) and becomes lymphoid at stage 5 (50–60 dph) correlating with an increase of T-cell marker transcripts. At this stage, a clear zonation into a RAG1(+) cortex and a RAG1(-) CD3ϵ(+) medulla was distinguished, indicating that T-cell maturation processes in ballan wrasse are similar to other teleosts. The higher abundance of CD4-1(+) compared to CD8β(+) cells in the thymus together with the apparent lack of CD8β(+) cells in gill, gut, and pharynx, where CD4-1(+) cells were identified, indicates that helper T-cells have a more prominent role during larval development compared to cytotoxic T-cells. As ballan wrasse lacks a stomach but has an exceptionally high IgM expression in the hindgut, we hypothesize that helper T-cells are crucial for activation and recruitment of IgM(+) B-cells and possibly other leukocytes to the gut during early development. Nutritional factors such as DHA/EPA, Zn and Se may lead to an earlier expression of certain T-cell markers as well as a larger size of the thymus, indicating an earlier onset of adaptive immunity. Including live feeds that supplies the larva with higher amounts of these nutrients can therefore be beneficial for ballan wrasse farming.
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spelling pubmed-101836032023-05-16 The thymus and T-cell ontogeny in ballan wrasse (Labrus bergylta) is nutritionally modelled Etayo, Angela Lie, Kai K. Bjelland, Reidun M. Hordvik, Ivar Øvergård, Aina-Cathrine Sæle, Øystein Front Immunol Immunology Marine fish larvae often experience high mortality unrelated to predation during early life stages, and farmed ballan wrasse (Labrus bergylta) is no exception. Knowing when the adaptive immune system is developed and fully functional, and how nutrition may modulate these processes is therefore of importance to establish effective prophylactic measures and will also extend the relatively limited knowledge on the immune system in lower vertebrates. The thymus anlage of ballan wrasse was found to be histologically visible for the first time at larval stage 3 (20–30 days post hatch, dph) and becomes lymphoid at stage 5 (50–60 dph) correlating with an increase of T-cell marker transcripts. At this stage, a clear zonation into a RAG1(+) cortex and a RAG1(-) CD3ϵ(+) medulla was distinguished, indicating that T-cell maturation processes in ballan wrasse are similar to other teleosts. The higher abundance of CD4-1(+) compared to CD8β(+) cells in the thymus together with the apparent lack of CD8β(+) cells in gill, gut, and pharynx, where CD4-1(+) cells were identified, indicates that helper T-cells have a more prominent role during larval development compared to cytotoxic T-cells. As ballan wrasse lacks a stomach but has an exceptionally high IgM expression in the hindgut, we hypothesize that helper T-cells are crucial for activation and recruitment of IgM(+) B-cells and possibly other leukocytes to the gut during early development. Nutritional factors such as DHA/EPA, Zn and Se may lead to an earlier expression of certain T-cell markers as well as a larger size of the thymus, indicating an earlier onset of adaptive immunity. Including live feeds that supplies the larva with higher amounts of these nutrients can therefore be beneficial for ballan wrasse farming. Frontiers Media S.A. 2023-05-01 /pmc/articles/PMC10183603/ /pubmed/37197651 http://dx.doi.org/10.3389/fimmu.2023.1166785 Text en Copyright © 2023 Etayo, Lie, Bjelland, Hordvik, Øvergård and Sæle https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Etayo, Angela
Lie, Kai K.
Bjelland, Reidun M.
Hordvik, Ivar
Øvergård, Aina-Cathrine
Sæle, Øystein
The thymus and T-cell ontogeny in ballan wrasse (Labrus bergylta) is nutritionally modelled
title The thymus and T-cell ontogeny in ballan wrasse (Labrus bergylta) is nutritionally modelled
title_full The thymus and T-cell ontogeny in ballan wrasse (Labrus bergylta) is nutritionally modelled
title_fullStr The thymus and T-cell ontogeny in ballan wrasse (Labrus bergylta) is nutritionally modelled
title_full_unstemmed The thymus and T-cell ontogeny in ballan wrasse (Labrus bergylta) is nutritionally modelled
title_short The thymus and T-cell ontogeny in ballan wrasse (Labrus bergylta) is nutritionally modelled
title_sort thymus and t-cell ontogeny in ballan wrasse (labrus bergylta) is nutritionally modelled
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10183603/
https://www.ncbi.nlm.nih.gov/pubmed/37197651
http://dx.doi.org/10.3389/fimmu.2023.1166785
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