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Application of a nitrifying bacterium Pseudomonas sp. HIB_D to reduce nitrogen waste in the Litopenaeus vannamei cultivation environment

Pseudomonas sp. HIB_D has been observed to have nitrification activity that can oxidize ammonia/ammonium to nitrite and nitrate. This bacterium was isolated from the aquaculture environment with Ancol Indonesian marine water sources. Pseudomonas sp. HIB_D was applied in the rearing water of Litopena...

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Detalles Bibliográficos
Autores principales: Hastuti, Yuni Puji, Siregar, Anggi, Fatma, Yuli Siti, Supriyono, Eddy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10183693/
https://www.ncbi.nlm.nih.gov/pubmed/37361879
http://dx.doi.org/10.1007/s10499-023-01123-6
Descripción
Sumario:Pseudomonas sp. HIB_D has been observed to have nitrification activity that can oxidize ammonia/ammonium to nitrite and nitrate. This bacterium was isolated from the aquaculture environment with Ancol Indonesian marine water sources. Pseudomonas sp. HIB_D was applied in the rearing water of Litopenaeus vannamei to decrease nitrogen pollution levels and support a sustainable cultivation environment. This present study was set up using a completely randomized design with four treatments and three replications, i.e., control (without bacterial application) and bacterial application with a cell density of 10(7), 10(8), and 10(9) CFU mL(−1) in a volume of 100 mL for 90 L seawater treatment. Thirty-six individuals of 15-day-old postlarvae L. vannamei (PL 15) (a density of 120 PL m(-2)) were used in this study for an 8-week rearing period. Based on the water quality analysis, the ammonia level under the bacterial applications was lower than in the control after 8-week cultivation. Nitrate levels increased at week 6 and decreased at week 8, specifically in 10(9) CFU mL(−1) treatment. L. vannamei with 10(9) CFU mL(−1) bacterial application had the best production performance with a survival rate of 94.33 ± 2.78%, an absolute length gain of 10.59 ± 0.22 cm, an absolute weight gain of 8.16 ± 0.33 g, a specific growth rate of 14.12% ± 0.01% day(−1), and a feed conversion ratio of 1.26 ± 0.03. The blood glucose level and total hemocyte count of shrimp under the bacterial application with a density of 10(9) CFU mL(−1) showed the highest level (30.71 ± 13.9 mg dL(−1)) and the lowest level (15 × 10(6) cells mL(−1)), respectively. The results showed that all treatments performed better than the control, in which the 10(9) CFU mL(−1) bacterial application was the best treatment.