Cargando…

The effects of patchouli alcohol and combination with cisplatin on proliferation, apoptosis and migration in B16F10 melanoma cells

Melanoma is a highly metastatic cancer with a low incidence rate, but a high mortality rate. Patchouli alcohol (PA), a tricyclic sesquiterpene, is considered the main active component in Pogostemon cablin Benth, which improves wound healing and has anti‐tumorigenic activity. However, the pharmacolog...

Descripción completa

Detalles Bibliográficos
Autores principales: Chang, Kai‐Fu, Lai, Hung‐Chih, Lee, Shan‐Chih, Huang, Xiao‐Fan, Huang, Ya‐Chih, Chou, Tien‐Erh, Hsiao, Chih‐Yen, Tsai, Nu‐Man
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10183711/
https://www.ncbi.nlm.nih.gov/pubmed/37038620
http://dx.doi.org/10.1111/jcmm.17745
_version_ 1785042016516702208
author Chang, Kai‐Fu
Lai, Hung‐Chih
Lee, Shan‐Chih
Huang, Xiao‐Fan
Huang, Ya‐Chih
Chou, Tien‐Erh
Hsiao, Chih‐Yen
Tsai, Nu‐Man
author_facet Chang, Kai‐Fu
Lai, Hung‐Chih
Lee, Shan‐Chih
Huang, Xiao‐Fan
Huang, Ya‐Chih
Chou, Tien‐Erh
Hsiao, Chih‐Yen
Tsai, Nu‐Man
author_sort Chang, Kai‐Fu
collection PubMed
description Melanoma is a highly metastatic cancer with a low incidence rate, but a high mortality rate. Patchouli alcohol (PA), a tricyclic sesquiterpene, is considered the main active component in Pogostemon cablin Benth, which improves wound healing and has anti‐tumorigenic activity. However, the pharmacological action of PA on anti‐melanoma remains unclear. Thus, the present study aimed to investigate the role of PA in the proliferation, cell cycle, apoptosis and migration of melanoma cells. These results indicated that PA selectively inhibited the proliferation of B16F10 cells in a dose‐ and time‐dependent manner. It induced cell cycle arrest at the G(0)/G(1) phase and typical morphological changes in apoptosis, such as chromatin condensation, DNA fragmentation and apoptotic bodies. In addition, PA reduced the migratory ability of B16F10 cells by upregulating E‐cadherin and downregulating p‐Smad2/3, vimentin, MMP‐2 and MMP‐9 expression. PA was also found to strongly suppress tumour growth in vivo. Furthermore, PA combined with cisplatin synergistically inhibited colony formation and migration of B16F10 cells and attenuated the development of resistance to treatment. Therefore, the results of this study indicate that PA may play a pivotal role in inducing apoptosis and reducing the migration of melanoma cells, and may thus be a potential candidate for melanoma treatment.
format Online
Article
Text
id pubmed-10183711
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-101837112023-05-16 The effects of patchouli alcohol and combination with cisplatin on proliferation, apoptosis and migration in B16F10 melanoma cells Chang, Kai‐Fu Lai, Hung‐Chih Lee, Shan‐Chih Huang, Xiao‐Fan Huang, Ya‐Chih Chou, Tien‐Erh Hsiao, Chih‐Yen Tsai, Nu‐Man J Cell Mol Med Original Articles Melanoma is a highly metastatic cancer with a low incidence rate, but a high mortality rate. Patchouli alcohol (PA), a tricyclic sesquiterpene, is considered the main active component in Pogostemon cablin Benth, which improves wound healing and has anti‐tumorigenic activity. However, the pharmacological action of PA on anti‐melanoma remains unclear. Thus, the present study aimed to investigate the role of PA in the proliferation, cell cycle, apoptosis and migration of melanoma cells. These results indicated that PA selectively inhibited the proliferation of B16F10 cells in a dose‐ and time‐dependent manner. It induced cell cycle arrest at the G(0)/G(1) phase and typical morphological changes in apoptosis, such as chromatin condensation, DNA fragmentation and apoptotic bodies. In addition, PA reduced the migratory ability of B16F10 cells by upregulating E‐cadherin and downregulating p‐Smad2/3, vimentin, MMP‐2 and MMP‐9 expression. PA was also found to strongly suppress tumour growth in vivo. Furthermore, PA combined with cisplatin synergistically inhibited colony formation and migration of B16F10 cells and attenuated the development of resistance to treatment. Therefore, the results of this study indicate that PA may play a pivotal role in inducing apoptosis and reducing the migration of melanoma cells, and may thus be a potential candidate for melanoma treatment. John Wiley and Sons Inc. 2023-04-10 /pmc/articles/PMC10183711/ /pubmed/37038620 http://dx.doi.org/10.1111/jcmm.17745 Text en © 2023 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Chang, Kai‐Fu
Lai, Hung‐Chih
Lee, Shan‐Chih
Huang, Xiao‐Fan
Huang, Ya‐Chih
Chou, Tien‐Erh
Hsiao, Chih‐Yen
Tsai, Nu‐Man
The effects of patchouli alcohol and combination with cisplatin on proliferation, apoptosis and migration in B16F10 melanoma cells
title The effects of patchouli alcohol and combination with cisplatin on proliferation, apoptosis and migration in B16F10 melanoma cells
title_full The effects of patchouli alcohol and combination with cisplatin on proliferation, apoptosis and migration in B16F10 melanoma cells
title_fullStr The effects of patchouli alcohol and combination with cisplatin on proliferation, apoptosis and migration in B16F10 melanoma cells
title_full_unstemmed The effects of patchouli alcohol and combination with cisplatin on proliferation, apoptosis and migration in B16F10 melanoma cells
title_short The effects of patchouli alcohol and combination with cisplatin on proliferation, apoptosis and migration in B16F10 melanoma cells
title_sort effects of patchouli alcohol and combination with cisplatin on proliferation, apoptosis and migration in b16f10 melanoma cells
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10183711/
https://www.ncbi.nlm.nih.gov/pubmed/37038620
http://dx.doi.org/10.1111/jcmm.17745
work_keys_str_mv AT changkaifu theeffectsofpatchoulialcoholandcombinationwithcisplatinonproliferationapoptosisandmigrationinb16f10melanomacells
AT laihungchih theeffectsofpatchoulialcoholandcombinationwithcisplatinonproliferationapoptosisandmigrationinb16f10melanomacells
AT leeshanchih theeffectsofpatchoulialcoholandcombinationwithcisplatinonproliferationapoptosisandmigrationinb16f10melanomacells
AT huangxiaofan theeffectsofpatchoulialcoholandcombinationwithcisplatinonproliferationapoptosisandmigrationinb16f10melanomacells
AT huangyachih theeffectsofpatchoulialcoholandcombinationwithcisplatinonproliferationapoptosisandmigrationinb16f10melanomacells
AT choutienerh theeffectsofpatchoulialcoholandcombinationwithcisplatinonproliferationapoptosisandmigrationinb16f10melanomacells
AT hsiaochihyen theeffectsofpatchoulialcoholandcombinationwithcisplatinonproliferationapoptosisandmigrationinb16f10melanomacells
AT tsainuman theeffectsofpatchoulialcoholandcombinationwithcisplatinonproliferationapoptosisandmigrationinb16f10melanomacells
AT changkaifu effectsofpatchoulialcoholandcombinationwithcisplatinonproliferationapoptosisandmigrationinb16f10melanomacells
AT laihungchih effectsofpatchoulialcoholandcombinationwithcisplatinonproliferationapoptosisandmigrationinb16f10melanomacells
AT leeshanchih effectsofpatchoulialcoholandcombinationwithcisplatinonproliferationapoptosisandmigrationinb16f10melanomacells
AT huangxiaofan effectsofpatchoulialcoholandcombinationwithcisplatinonproliferationapoptosisandmigrationinb16f10melanomacells
AT huangyachih effectsofpatchoulialcoholandcombinationwithcisplatinonproliferationapoptosisandmigrationinb16f10melanomacells
AT choutienerh effectsofpatchoulialcoholandcombinationwithcisplatinonproliferationapoptosisandmigrationinb16f10melanomacells
AT hsiaochihyen effectsofpatchoulialcoholandcombinationwithcisplatinonproliferationapoptosisandmigrationinb16f10melanomacells
AT tsainuman effectsofpatchoulialcoholandcombinationwithcisplatinonproliferationapoptosisandmigrationinb16f10melanomacells