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Deficient butyrate-producing capacity in the gut microbiome is associated with bacterial network disturbances and fatigue symptoms in ME/CFS
Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is characterized by unexplained debilitating fatigue, cognitive dysfunction, gastrointestinal disturbances, and orthostatic intolerance. Here, we report a multi-omic analysis of a geographically diverse cohort of 106 cases and 91 healthy co...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10183837/ https://www.ncbi.nlm.nih.gov/pubmed/36758522 http://dx.doi.org/10.1016/j.chom.2023.01.004 |
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author | Guo, Cheng Che, Xiaoyu Briese, Thomas Ranjan, Amit Allicock, Orchid Yates, Rachel A. Cheng, Aaron March, Dana Hornig, Mady Komaroff, Anthony L. Levine, Susan Bateman, Lucinda Vernon, Suzanne D. Klimas, Nancy G. Montoya, Jose G. Peterson, Daniel L. Lipkin, W. Ian Williams, Brent L. |
author_facet | Guo, Cheng Che, Xiaoyu Briese, Thomas Ranjan, Amit Allicock, Orchid Yates, Rachel A. Cheng, Aaron March, Dana Hornig, Mady Komaroff, Anthony L. Levine, Susan Bateman, Lucinda Vernon, Suzanne D. Klimas, Nancy G. Montoya, Jose G. Peterson, Daniel L. Lipkin, W. Ian Williams, Brent L. |
author_sort | Guo, Cheng |
collection | PubMed |
description | Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is characterized by unexplained debilitating fatigue, cognitive dysfunction, gastrointestinal disturbances, and orthostatic intolerance. Here, we report a multi-omic analysis of a geographically diverse cohort of 106 cases and 91 healthy controls that revealed differences in gut microbiome diversity, abundances, functional pathways, and interactions. Faecalibacterium prausnitzii and Eubacterium rectale, which are both recognized as abundant, health-promoting butyrate producers in the human gut, were reduced in ME/CFS. Functional metagenomics, qPCR, and metabolomics of fecal short-chain fatty acids confirmed a deficient microbial capacity for butyrate synthesis. Microbiome-based machine learning classifier models were robust to geographic variation and generalizable in a validation cohort. The abundance of Faecalibacterium prausnitzii was inversely associated with fatigue severity. These findings demonstrate the functional nature of gut dysbiosis and the underlying microbial network disturbance in ME/CFS, providing possible targets for disease classification and therapeutic trials. |
format | Online Article Text |
id | pubmed-10183837 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
record_format | MEDLINE/PubMed |
spelling | pubmed-101838372023-05-15 Deficient butyrate-producing capacity in the gut microbiome is associated with bacterial network disturbances and fatigue symptoms in ME/CFS Guo, Cheng Che, Xiaoyu Briese, Thomas Ranjan, Amit Allicock, Orchid Yates, Rachel A. Cheng, Aaron March, Dana Hornig, Mady Komaroff, Anthony L. Levine, Susan Bateman, Lucinda Vernon, Suzanne D. Klimas, Nancy G. Montoya, Jose G. Peterson, Daniel L. Lipkin, W. Ian Williams, Brent L. Cell Host Microbe Article Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is characterized by unexplained debilitating fatigue, cognitive dysfunction, gastrointestinal disturbances, and orthostatic intolerance. Here, we report a multi-omic analysis of a geographically diverse cohort of 106 cases and 91 healthy controls that revealed differences in gut microbiome diversity, abundances, functional pathways, and interactions. Faecalibacterium prausnitzii and Eubacterium rectale, which are both recognized as abundant, health-promoting butyrate producers in the human gut, were reduced in ME/CFS. Functional metagenomics, qPCR, and metabolomics of fecal short-chain fatty acids confirmed a deficient microbial capacity for butyrate synthesis. Microbiome-based machine learning classifier models were robust to geographic variation and generalizable in a validation cohort. The abundance of Faecalibacterium prausnitzii was inversely associated with fatigue severity. These findings demonstrate the functional nature of gut dysbiosis and the underlying microbial network disturbance in ME/CFS, providing possible targets for disease classification and therapeutic trials. 2023-02-08 /pmc/articles/PMC10183837/ /pubmed/36758522 http://dx.doi.org/10.1016/j.chom.2023.01.004 Text en https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Article Guo, Cheng Che, Xiaoyu Briese, Thomas Ranjan, Amit Allicock, Orchid Yates, Rachel A. Cheng, Aaron March, Dana Hornig, Mady Komaroff, Anthony L. Levine, Susan Bateman, Lucinda Vernon, Suzanne D. Klimas, Nancy G. Montoya, Jose G. Peterson, Daniel L. Lipkin, W. Ian Williams, Brent L. Deficient butyrate-producing capacity in the gut microbiome is associated with bacterial network disturbances and fatigue symptoms in ME/CFS |
title | Deficient butyrate-producing capacity in the gut microbiome is associated with bacterial network disturbances and fatigue symptoms in ME/CFS |
title_full | Deficient butyrate-producing capacity in the gut microbiome is associated with bacterial network disturbances and fatigue symptoms in ME/CFS |
title_fullStr | Deficient butyrate-producing capacity in the gut microbiome is associated with bacterial network disturbances and fatigue symptoms in ME/CFS |
title_full_unstemmed | Deficient butyrate-producing capacity in the gut microbiome is associated with bacterial network disturbances and fatigue symptoms in ME/CFS |
title_short | Deficient butyrate-producing capacity in the gut microbiome is associated with bacterial network disturbances and fatigue symptoms in ME/CFS |
title_sort | deficient butyrate-producing capacity in the gut microbiome is associated with bacterial network disturbances and fatigue symptoms in me/cfs |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10183837/ https://www.ncbi.nlm.nih.gov/pubmed/36758522 http://dx.doi.org/10.1016/j.chom.2023.01.004 |
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