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Stimulatory effects of Lycium shawii on human melanocyte proliferation, migration, and melanogenesis: In vitro and in silico studies

There is no first-line treatment for vitiligo, a skin disease characterized by a lack of melanin produced by the melanocytes, resulting in an urgent demand for new therapeutic drugs capable of stimulating melanocyte functions, including melanogenesis. In this study, traditional medicinal plant extra...

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Autores principales: Alghamdi, Khalid, Alehaideb, Zeyad, Kumar, Ashok, Al-Eidi, Hamad, Alghamdi, Sahar S., Suliman, Rasha, Ali, Rizwan, Almourfi, Feras, Alghamdi, Saleh M., Boudjelal, Mohamed, Matou-Nasri, Sabine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10184183/
https://www.ncbi.nlm.nih.gov/pubmed/37197407
http://dx.doi.org/10.3389/fphar.2023.1169812
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author Alghamdi, Khalid
Alehaideb, Zeyad
Kumar, Ashok
Al-Eidi, Hamad
Alghamdi, Sahar S.
Suliman, Rasha
Ali, Rizwan
Almourfi, Feras
Alghamdi, Saleh M.
Boudjelal, Mohamed
Matou-Nasri, Sabine
author_facet Alghamdi, Khalid
Alehaideb, Zeyad
Kumar, Ashok
Al-Eidi, Hamad
Alghamdi, Sahar S.
Suliman, Rasha
Ali, Rizwan
Almourfi, Feras
Alghamdi, Saleh M.
Boudjelal, Mohamed
Matou-Nasri, Sabine
author_sort Alghamdi, Khalid
collection PubMed
description There is no first-line treatment for vitiligo, a skin disease characterized by a lack of melanin produced by the melanocytes, resulting in an urgent demand for new therapeutic drugs capable of stimulating melanocyte functions, including melanogenesis. In this study, traditional medicinal plant extracts were tested for cultured human melanocyte proliferation, migration, and melanogenesis using MTT, scratch wound-healing assays, transmission electron microscopy, immunofluorescence staining, and Western blot technology. Of the methanolic extracts, Lycium shawii L. (L. shawii) extract increased melanocyte proliferation at low concentrations and modulated melanocyte migration. At the lowest tested concentration (i.e., 7.8 μg/mL), the L. shawii methanolic extract promoted melanosome formation, maturation, and enhanced melanin production, which was associated with the upregulation of microphthalmia-associated transcription factor (MITF), tyrosinase, tyrosinase-related protein (TRP)-1 and TRP-2 melanogenesis-related proteins, and melanogenesis-related proteins. After the chemical analysis and L. shawii extract-derived metabolite identification, the in silico studies revealed the molecular interactions between Metabolite 5, identified as apigenin (4,5,6-trihydroxyflavone), and the copper active site of tyrosinase, predicting enhanced tyrosinase activity and subsequent melanin formation. In conclusion, L. shawii methanolic extract stimulates melanocyte functions, including melanin production, and its derivative Metabolite 5 enhances tyrosinase activity, suggesting further investigation of the L. shawii extract-derived Metabolite 5 as a potential natural drug for vitiligo treatment.
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spelling pubmed-101841832023-05-16 Stimulatory effects of Lycium shawii on human melanocyte proliferation, migration, and melanogenesis: In vitro and in silico studies Alghamdi, Khalid Alehaideb, Zeyad Kumar, Ashok Al-Eidi, Hamad Alghamdi, Sahar S. Suliman, Rasha Ali, Rizwan Almourfi, Feras Alghamdi, Saleh M. Boudjelal, Mohamed Matou-Nasri, Sabine Front Pharmacol Pharmacology There is no first-line treatment for vitiligo, a skin disease characterized by a lack of melanin produced by the melanocytes, resulting in an urgent demand for new therapeutic drugs capable of stimulating melanocyte functions, including melanogenesis. In this study, traditional medicinal plant extracts were tested for cultured human melanocyte proliferation, migration, and melanogenesis using MTT, scratch wound-healing assays, transmission electron microscopy, immunofluorescence staining, and Western blot technology. Of the methanolic extracts, Lycium shawii L. (L. shawii) extract increased melanocyte proliferation at low concentrations and modulated melanocyte migration. At the lowest tested concentration (i.e., 7.8 μg/mL), the L. shawii methanolic extract promoted melanosome formation, maturation, and enhanced melanin production, which was associated with the upregulation of microphthalmia-associated transcription factor (MITF), tyrosinase, tyrosinase-related protein (TRP)-1 and TRP-2 melanogenesis-related proteins, and melanogenesis-related proteins. After the chemical analysis and L. shawii extract-derived metabolite identification, the in silico studies revealed the molecular interactions between Metabolite 5, identified as apigenin (4,5,6-trihydroxyflavone), and the copper active site of tyrosinase, predicting enhanced tyrosinase activity and subsequent melanin formation. In conclusion, L. shawii methanolic extract stimulates melanocyte functions, including melanin production, and its derivative Metabolite 5 enhances tyrosinase activity, suggesting further investigation of the L. shawii extract-derived Metabolite 5 as a potential natural drug for vitiligo treatment. Frontiers Media S.A. 2023-04-24 /pmc/articles/PMC10184183/ /pubmed/37197407 http://dx.doi.org/10.3389/fphar.2023.1169812 Text en Copyright © 2023 Alghamdi, Alehaideb, Kumar, Al-Eidi, Alghamdi, Suliman, Ali, Almourfi, Alghamdi, Boudjelal and Matou-Nasri. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Alghamdi, Khalid
Alehaideb, Zeyad
Kumar, Ashok
Al-Eidi, Hamad
Alghamdi, Sahar S.
Suliman, Rasha
Ali, Rizwan
Almourfi, Feras
Alghamdi, Saleh M.
Boudjelal, Mohamed
Matou-Nasri, Sabine
Stimulatory effects of Lycium shawii on human melanocyte proliferation, migration, and melanogenesis: In vitro and in silico studies
title Stimulatory effects of Lycium shawii on human melanocyte proliferation, migration, and melanogenesis: In vitro and in silico studies
title_full Stimulatory effects of Lycium shawii on human melanocyte proliferation, migration, and melanogenesis: In vitro and in silico studies
title_fullStr Stimulatory effects of Lycium shawii on human melanocyte proliferation, migration, and melanogenesis: In vitro and in silico studies
title_full_unstemmed Stimulatory effects of Lycium shawii on human melanocyte proliferation, migration, and melanogenesis: In vitro and in silico studies
title_short Stimulatory effects of Lycium shawii on human melanocyte proliferation, migration, and melanogenesis: In vitro and in silico studies
title_sort stimulatory effects of lycium shawii on human melanocyte proliferation, migration, and melanogenesis: in vitro and in silico studies
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10184183/
https://www.ncbi.nlm.nih.gov/pubmed/37197407
http://dx.doi.org/10.3389/fphar.2023.1169812
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